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Study · In vitro (cells) · 2025

6 results

The mitochondrial protectant SS31 optimized decellularized Wharton's jelly scaffold improves allogeneic chondrocyte implantation-mediated articular cartilage repair.

Wang C, Li H, Li F, Yang Y, Xu Z, Gao T, Li R, Zhang R, Mu Y, Guo Z, Guo Q, Liu S

Journal of orthopaedic translation15 Apr 2025Cited by 4 (Europe PMC)

PMID40291636 (opens in a new tab)PMCIDPMC12032180 (opens in a new tab)DOI10.1016/j.jot.2025.03.023 (opens in a new tab)PepBankPUB-40291636
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Summary

BackgroundThe process of allogeneic chondrocyte implantation entails obtaining donor chondrocytes, culturing them in a medium enriched with growth factors, and then introducing them-either individually or in conjunction with biocompatible scaffolds-into areas of cartilage damage. While promising, this approach is hindered by mitochondrial dysfunction in the implanted chondrocytes.MethodsThis research introduced an innovative approach by creating a new type of scaffold derived from Decellularized Umbilical Cord Wharton's Jelly (DUCWJ) extracted from human umbilical cords. The scaffold was manufactured using procedures involving decellularization and lyophilization. The resulting scaffold demonstrated superior characteristics, including high porosity, hydrophilic properties, and excellent biocompatibility. To enhance its function, SS31 peptides, known for their mitochondrial-protective properties, were chemically bonded to the scaffold surface, creating an SS31@DUCWJ system. This system aims to protect chondrocytes and regulate the mitochondrial respiratory chain (MRC), thereby improving cartilage repair mediated by allogeneic chondrocyte implantation.ResultsIn vitro studies have shown that SS31 effectively attenuates metabolic dysfunction, extracellular matrix degradation, oxidative stress, inflammation, and mitochondrial damage induced by serial cell passages. Complementary in vivo experiments showed that the SS31@DUCWJ scaffold promoted regeneration of healthy articular cartilage in femoral condylar defects in rabbits.ConclusionsThis SS31-modified porous decellularized scaffold represents an innovative biomaterial with anti-inflammatory properties and targeted mitochondrial regulation. It offers a promising new approach for treating articular cartilage injuries.The translational potential of this articleOur study was the first to successfully load the mitochondrial protectant SS31 onto a DUCWJ hydrogel scaffold for localized drug delivery. This method is highly efficacious in repairing cartilage defects and offers a promising new avenue for the treatment of such conditions.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study6

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In cell culture, SS31 attenuated metabolic dysfunction induced by serial cell passages.

    In plain wordsIn lab tests, SS31 reduced metabolic problems in cells grown through many passages.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseMetabolic dysfunction induced by serial passages
    Study

    n: not reported in source

    Outcome group
    Mitochondrial function · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In vitro studies have shown that SS31 effectively attenuates metabolic dysfunction, extracellular matrix degradation, oxidative stress, inflammation, and mitochondrial damage induced by serial cell passages.
  2. 02LabIn vitro (cells)Efficacy outcome

    In cell culture, SS31 attenuated extracellular matrix degradation induced by serial cell passages.

    In plain wordsIn lab tests, SS31 reduced breakdown of the cell scaffold material in cells grown through many passages.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseExtracellular matrix degradation
    Study

    n: not reported in source

    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In vitro studies have shown that SS31 effectively attenuates metabolic dysfunction, extracellular matrix degradation, oxidative stress, inflammation, and mitochondrial damage induced by serial cell passages.
  3. 03LabIn vitro (cells)Efficacy outcome

    In cell culture, SS31 attenuated oxidative stress induced by serial cell passages.

    In plain wordsIn lab tests, SS31 reduced oxidative stress in cells grown through many passages.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseOxidative stress
    Study

    n: not reported in source

    Outcome group
    Oxidative stress · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In vitro studies have shown that SS31 effectively attenuates metabolic dysfunction, extracellular matrix degradation, oxidative stress, inflammation, and mitochondrial damage induced by serial cell passages.
  4. 04LabIn vitro (cells)Efficacy outcome

    In cell culture, SS31 attenuated inflammation induced by serial cell passages.

    In plain wordsIn lab tests, SS31 reduced inflammation in cells grown through many passages.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseInflammation
    Study

    n: not reported in source

    Outcome group
    Inflammation markers · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In vitro studies have shown that SS31 effectively attenuates metabolic dysfunction, extracellular matrix degradation, oxidative stress, inflammation, and mitochondrial damage induced by serial cell passages.
  5. 05LabIn vitro (cells)Efficacy outcome

    In cell culture, SS31 attenuated mitochondrial damage induced by serial cell passages.

    In plain wordsIn lab tests, SS31 reduced mitochondrial damage in cells grown through many passages.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseMitochondrial damage
    Study

    n: not reported in source

    Outcome group
    Mitochondrial function · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In vitro studies have shown that SS31 effectively attenuates metabolic dysfunction, extracellular matrix degradation, oxidative stress, inflammation, and mitochondrial damage induced by serial cell passages.

Studies that did not test the peptide alone1

In this study, Elamipretide (SS-31) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Elamipretide (SS-31).

  1. 06AnimalNot a test of Elamipretide (SS-31) aloneAnimal study (in vivo)Efficacy outcome

    In rabbits with femoral condylar defects, the SS31@DUCWJ scaffold (SS31 bonded to a decellularized Wharton's jelly scaffold) promoted regeneration of healthy articular cartilage.

    In plain wordsIn rabbits, a scaffold carrying SS31 helped cartilage regrow in knee joint defects. The effect of SS31 alone was not tested here.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseArticular cartilage regeneration in femoral condylar defects
    Study

    rabbitn: not reported in source

    Femoral condylar defects
    Outcome group
    Bone and joint · Musculoskeletal

    Source sentences

    Summary
    Complementary in vivo experiments showed that the SS31@DUCWJ scaffold promoted regeneration of healthy articular cartilage in femoral condylar defects in rabbits.
    1 context sentence
    Summary · context
    To enhance its function, SS31 peptides, known for their mitochondrial-protective properties, were chemically bonded to the scaffold surface, creating an SS31@DUCWJ system.

    In this study, Elamipretide (SS-31) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Elamipretide (SS-31).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 08:19 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:47 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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