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Study · In vitro (cells) · sheep · 2025

2 results

Influence of neurokinin B, dynorphin A and kisspeptin-10 on in vitro gonadotropin secretion by anterior pituitary cells isolated from pubescent ewes.

Szysiak N, Kosior-Korzecka U, Longo V, Patkowski K, Greguła-Kania M, Nowakiewicz A, Bochniarz M, Junkuszew A

Journal of veterinary research31 Jan 2025Cited by 2 (Europe PMC)

PMID40144066 (opens in a new tab)PMCIDPMC11936093 (opens in a new tab)DOI10.2478/jvetres-2025-0003 (opens in a new tab)PepBankPUB-40144066
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Summary

IntroductionThe hypothalamic neuropeptides kisspeptin-10 (KiSS-10), neurokinin B (NKB), and dynorphin A (Dyn A) play roles in the endocrine regulation of the hypothalamic-pituitary-ovarian (HPO) axis in puberty. Livestock's timely attainment of sexual maturity increases reproductive efficiency and raises profitability. The pituitary relationship between these neuropeptides and gonadotropins in puberty in ewes being undercharacterised. The aim of the study was to analyse their direct effect on gonadotropin secretion by pituitary cells isolated from pubescent ewes.Material and methodsCells were incubated in McCoy's 5A medium, either without neuropeptides (as the control) or with 10-11, 10-10, 10-9, 10-8 and 10-7 M of KiSS-10, NKB and Dyn A. After 4, 12 and 24 h, the luteinising hormone (LH) and follicle-stimulating hormone (FSH) concentrations were analysed by ELISA using species-specific antibodies.ResultsGreater LH and FSH secretion was observed after the 4-24 h exposure to respective 10-11-10-8 M and 10-11-10-7 M concentrations of KiSS-10. Moreover, NKB and Dyn A applied in the concentration range elevated the secretion of both LH and FSH throughout the experiment. Dynorphin A had the most significant effect on gonadotropin secretion at all the concentrations used. In contrast, the most pronounced dose-dependent neuropeptide effect throughout the experiment on the FSH secretion was attributed to NKB.ConclusionKisspeptin-10, NKB and Dyn A had a direct impact on gonadotropin secretion by ovine pituitary cells. However, a detailed explanation of their role in gonadotropin secretion by the anterior pituitary gland in sheep and of their impact on the regulation of the HPO axis during sexual maturation or in the pathomechanism of delayed puberty require further studies.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In primary pituitary cells from pubescent ewes, kisspeptin‑10 increased luteinising hormone secretion after 4-24 h exposure compared with the control.

    In plain wordsIn cells from sheep pituitary glands, kisspeptin‑10 increased release of luteinising hormone compared with untreated cells.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseLH secretion
    Study

    sheepn: not reported in source

    Primary anterior pituitary cells from pubescent ewes
    Comparator
    Control (no neuropeptide)
    Outcome group
    Sex hormones and fertility · Endocrine and hormones
    Studied dose
    10^-11 to 10^-8 M · 4-24 hAs given in this study (sheep).

    Source sentences

    Summary
    Greater LH and FSH secretion was observed after the 4-24 h exposure to respective 10-11-10-8 M and 10-11-10-7 M concentrations of KiSS-10.
    1 context sentence
    Summary · context
    Cells were incubated in McCoy's 5A medium, either without neuropeptides (as the control) or with 10-11, 10-10, 10-9, 10-8 and 10-7 M of KiSS-10, NKB and Dyn A. After 4, 12 and 24 h, the luteinising hormone (LH) and follicle-stimulating hormone (FSH) concentrations were analysed by ELISA using species-specific antibodies.
  2. 02LabIn vitro (cells)Activity measurement

    In primary pituitary cells from pubescent ewes, kisspeptin‑10 increased follicle-stimulating hormone secretion after 4-24 h exposure compared with the control.

    In plain wordsIn cells from sheep pituitary glands, kisspeptin‑10 increased release of follicle-stimulating hormone compared with untreated cells.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseFSH secretion
    Study

    sheepn: not reported in source

    Primary anterior pituitary cells from pubescent ewes
    Comparator
    Control (no neuropeptide)
    Outcome group
    Sex hormones and fertility · Endocrine and hormones
    Studied dose
    10^-11 to 10^-7 M · 4-24 hAs given in this study (sheep).

    Source sentences

    Summary
    Greater LH and FSH secretion was observed after the 4-24 h exposure to respective 10-11-10-8 M and 10-11-10-7 M concentrations of KiSS-10.
    1 context sentence
    Summary · context
    Cells were incubated in McCoy's 5A medium, either without neuropeptides (as the control) or with 10-11, 10-10, 10-9, 10-8 and 10-7 M of KiSS-10, NKB and Dyn A. After 4, 12 and 24 h, the luteinising hormone (LH) and follicle-stimulating hormone (FSH) concentrations were analysed by ELISA using species-specific antibodies.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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