Potential Role of High-Intensity Interval Training-Induced Increase in Humanin Levels for the Management of Type 2 Diabetes.

Soltany A, Daryanoosh F, Gholampour F, Sadat Hosseini N, Khoramipour K

Journal of cellular and molecular medicine1 Feb 2025Cited by 2 (Europe PMC)

PMID39936487PMCIDPMC11815479DOI10.1111/jcmm.70396PepBankPUB-39936487

Summary

This study investigated the effect of 8 weeks of high-intensity interval training (HIIT) on oxidative stress, inflammation, and apoptosis in rats with type 2 diabetes (T2D), focusing on the role of the Humanin (HN). In this study, 28 male Wistar rats were assigned to one of four groups: healthy control (CO), diabetes control (T2D), exercise (EX), and diabetes + exercise (T2D + EX). After diabetes induction (2-month high-fat diet and injection of 35 mg/kg streptozotocin), the animals in the EX and T2D + EX groups underwent an 8-week HIIT protocol (4-10, interval of 80%-100% of maximum speed). HOMA-IR, fasting blood glucose, and HN levels were measured in the serum. The expression of HN, Bax, Bcl-2, CAT, GPx, MDA, TNFα, and IL-10 was measured in the soleus muscle. Our results showed that the serum level of HN and the muscle levels of IL-10, SOD, CAT, and Bax were higher in the T2D + EX group than in the T2D group. However, the HOMA-IR index and the muscle levels of MDA, TNFα, and Bcl-2 were lower in the T2D + EX group than in the T2D group. Muscle levels of HN and GPx showed no significant difference between the T2D + EX and T2D groups. The result of Pearson analysis showed a significant correlation between HN and MDA, SOD, Bax and Bcl-2. This study provides evidence that there is a correlation between serum Humanin levels and HIIT. HIIT benefits T2D rats by reducing inflammation and oxidative stress. Given Humanin's established involvement in inflammation and oxidative stress, it is possible that the benefits of HIIT on T2D rats are mediated by humanin.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study3

No result from this study comes from a test of the peptide.

Natural levels measured3

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01AnimalEndogenous levelAnimal study (in vivo)Other
In rats with type 2 diabetes, serum Humanin was higher in the HIIT group than in the diabetic control group.
In plain wordsIn rats with type 2 diabetes, interval training raised the body's own Humanin in the blood.
Peptide
Humanin
Outcome
IncreaseSerum Humanin after HIIT
Study
ratn: not reported in source
Wistar rats with type 2 diabetes (high-fat diet and streptozotocin)
Comparator
Diabetic rats without exercise
Outcome group
Exercise and physical function · Metabolic and weight
Source sentences
Summary
Our results showed that the serum level of HN and the muscle levels of IL-10, SOD, CAT, and Bax were higher in the T2D + EX group than in the T2D group.
2 context sentences(design, population, supporting numbers)
Summary · context
This study investigated the effect of 8 weeks of high-intensity interval training (HIIT) on oxidative stress, inflammation, and apoptosis in rats with type 2 diabetes (T2D), focusing on the role of the Humanin (HN).
Summary · context
In this study, 28 male Wistar rats were assigned to one of four groups: healthy control (CO), diabetes control (T2D), exercise (EX), and diabetes + exercise (T2D + EX).
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-3993648701
02AnimalEndogenous levelAnimal study (in vivo)Other
In rats with type 2 diabetes, muscle Humanin levels did not differ between the HIIT group and the diabetic control group.
In plain wordsIn rats with type 2 diabetes, interval training did not change the body's own Humanin in muscle.
Peptide
Humanin
Outcome
No significant changeMuscle Humanin expression after HIIT
Study
ratn: not reported in source
Wistar rats with type 2 diabetes (high-fat diet and streptozotocin)
Comparator
Diabetic rats without exercise
Outcome group
Exercise and physical function · Metabolic and weight
Source sentences
Summary
Muscle levels of HN and GPx showed no significant difference between the T2D + EX and T2D groups.
2 context sentences(design, population, supporting numbers)
Summary · context
This study investigated the effect of 8 weeks of high-intensity interval training (HIIT) on oxidative stress, inflammation, and apoptosis in rats with type 2 diabetes (T2D), focusing on the role of the Humanin (HN).
Summary · context
In this study, 28 male Wistar rats were assigned to one of four groups: healthy control (CO), diabetes control (T2D), exercise (EX), and diabetes + exercise (T2D + EX).
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-3993648702
03AnimalEndogenous levelAnimal study (in vivo)Other
In rats with type 2 diabetes, Humanin correlated significantly with MDA, SOD, Bax and Bcl-2.
In plain wordsIn rats with type 2 diabetes, the body's own Humanin levels were linked to markers of oxidative stress and cell death.
Peptide
Humanin
Outcome
Not applicableCorrelation of Humanin with MDA, SOD, Bax and Bcl-2
Study
ratn: not reported in source
Wistar rats with type 2 diabetes (high-fat diet and streptozotocin)
Outcome group
Exercise and physical function · Metabolic and weight
Source sentences
Summary
The result of Pearson analysis showed a significant correlation between HN and MDA, SOD, Bax and Bcl-2.
2 context sentences(design, population, supporting numbers)
Summary · context
This study investigated the effect of 8 weeks of high-intensity interval training (HIIT) on oxidative stress, inflammation, and apoptosis in rats with type 2 diabetes (T2D), focusing on the role of the Humanin (HN).
Summary · context
In this study, 28 male Wistar rats were assigned to one of four groups: healthy control (CO), diabetes control (T2D), exercise (EX), and diabetes + exercise (T2D + EX).
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-3993648703

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

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