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Study · Animal study (in vivo) · rat · 2025

9 results

Humanin reduces nucleus pulposus cells ferroptosis to alleviate intervertebral disc degeneration: An in vitro and in vivo study.

Zhu D, Wang Z, Li Y, Chen S, Kang X

Journal of orthopaedic translation21 Jan 2025Cited by 7 (Europe PMC)

PMID39902261 (opens in a new tab)PMCIDPMC11788685 (opens in a new tab)DOI10.1016/j.jot.2024.12.002 (opens in a new tab)PepBankPUB-39902261
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: CC BY-NC-ND. Results still quote short attributed sentences.

Results from this study9

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In rats with annulus fibrosus puncture-induced disc degeneration, HN treatment ameliorated disc degeneration progression.

    In plain wordsIn rats with induced disc degeneration, Humanin treatment slowed the disease.

    Peptide
    Humanin
    Outcome
    DecreaseIDD progression (MRI and histology)
    Study

    ratn: not reported in source

    annulus fibrosus puncture IDD model
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Notably, in vivo rat experiments underscored the efficacy of HN treatment in ameliorating IDD progression induced by annulus fibrosus puncture.
    1 context sentence
    Summary · context
    The therapeutic efficacy of HN in rats was assessed via MRI and histological evaluation, alongside an exploration of the molecular underpinnings of HN's therapeutic actions in IDD management.
  2. 02LabIn vitro (cells)Mechanism

    In nucleus pulposus cells, exogenous Humanin triggered endogenous Humanin expression.

    In plain wordsIn cells, added Humanin switched on the cells' own Humanin production.

    Peptide
    Humanin
    Outcome
    IncreaseEndogenous HN expression after exogenous HN
    Study

    humann: not reported in source

    nucleus pulposus cells, erastin-induced ferroptosis
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    exogenous HN could trigger endogenous HN expression, impede the JAK2/STAT3 and NF-κB pathways, thereby mitigating erastin-induced ferroptosis in NP cells, contingent upon the upregulation of HSP27 expression.
    1 context sentence
    Summary · context
    The expression profile of HN in IDD was scrutinized utilizing human NP cell cultures and an IDD rat model (n = 5).
  3. 03LabIn vitro (cells)Efficacy outcome

    In nucleus pulposus cells, exogenous Humanin mitigated erastin-induced ferroptosis, depending on upregulation of HSP27 expression.

    In plain wordsIn cells, added Humanin reduced a form of cell death called ferroptosis, and this needed more HSP27.

    Peptide
    Humanin
    Outcome
    DecreaseErastin-induced ferroptosis
    Study

    humann: not reported in source

    nucleus pulposus cells, erastin-induced ferroptosis
    Outcome group
    Organ injury · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    mitigating erastin-induced ferroptosis in NP cells, contingent upon the upregulation of HSP27 expression.
    1 context sentence
    Summary · context
    The expression profile of HN in IDD was scrutinized utilizing human NP cell cultures and an IDD rat model (n = 5).
  4. 04LabIn vitro (cells)Mechanism

    In nucleus pulposus cells, exogenous Humanin impeded the JAK2/STAT3 and NF-κB pathways.

    In plain wordsIn cells, added Humanin dampened the JAK2/STAT3 and NF-κB signalling pathways.

    Peptide
    Humanin
    Outcome
    DecreaseJAK2/STAT3 and NF-κB pathway activity
    Study

    humann: not reported in source

    nucleus pulposus cells, erastin-induced ferroptosis
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    impede the JAK2/STAT3 and NF-κB pathways, thereby mitigating erastin-induced ferroptosis in NP cells, contingent upon the upregulation of HSP27 expression.
    1 context sentence
    Summary · context
    The expression profile of HN in IDD was scrutinized utilizing human NP cell cultures and an IDD rat model (n = 5).
  5. 05LabIn vitro (cells)Mechanism

    In nucleus pulposus cells, Humanin curbed mitochondrial reactive oxygen species generation.

    In plain wordsIn cells, Humanin lowered the production of reactive oxygen species by mitochondria.

    Peptide
    Humanin
    Outcome
    DecreaseMitochondrial ROS generation
    Study

    humann: not reported in source

    nucleus pulposus cells
    Outcome group
    Mitochondrial function · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    curbing mitochondrial reactive oxygen species (mtROS) generation and mtDNA leakage, consequently hindering mtDNA binding to TLR9 and subsequent activation of the NF-κB pathway.
    1 context sentence
    Summary · context
    The expression profile of HN in IDD was scrutinized utilizing human NP cell cultures and an IDD rat model (n = 5).
  6. 06LabIn vitro (cells)Mechanism

    In nucleus pulposus cells, Humanin curbed mtDNA leakage, hindering mtDNA binding to TLR9 and activation of the NF-κB pathway.

    In plain wordsIn cells, Humanin reduced leakage of mitochondrial DNA and the signal that follows it.

    Peptide
    Humanin
    Outcome
    DecreasemtDNA leakage and TLR9/NF-κB activation
    Study

    humann: not reported in source

    nucleus pulposus cells
    Outcome group
    Mitochondrial function · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    mtDNA leakage, consequently hindering mtDNA binding to TLR9 and subsequent activation of the NF-κB pathway.
    1 context sentence
    Summary · context
    The expression profile of HN in IDD was scrutinized utilizing human NP cell cultures and an IDD rat model (n = 5).

Natural levels measured3

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 07HumanEndogenous levelObservational studyOther

    In patients with intervertebral disc degeneration, Humanin expression was downregulated.

    In plain wordsIn people with disc degeneration, the body's own Humanin levels in disc tissue were lower.

    Peptide
    Humanin
    Outcome
    DecreaseHN expression in intervertebral disc degeneration
    Study

    n: not reported in source

    Outcome group
    Aging markers · Musculoskeletal

    Source sentences

    Summary
    This pioneering study unveiled a downregulation of HN expression in IDD patients, a finding corroborated through cell and rat IDD models.
    1 context sentence
    Summary · context
    The expression profile of HN in IDD was scrutinized utilizing human NP cell cultures and an IDD rat model (n = 5).

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

  2. 08AnimalEndogenous levelAnimal study (in vivo)Other

    In a rat model of intervertebral disc degeneration, Humanin expression was downregulated.

    In plain wordsIn rats with disc degeneration, natural Humanin levels were lower.

    Peptide
    Humanin
    Outcome
    DecreaseHN expression in rat disc degeneration
    Study

    ratn = 5

    annulus fibrosus puncture IDD model
    Outcome group
    Aging markers · Musculoskeletal

    Source sentences

    Summary
    This pioneering study unveiled a downregulation of HN expression in IDD patients, a finding corroborated through cell and rat IDD models.
    1 context sentence
    Summary · context
    The expression profile of HN in IDD was scrutinized utilizing human NP cell cultures and an IDD rat model (n = 5).

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

  3. 09LabEndogenous levelIn vitro (cells)Other

    In human nucleus pulposus cell cultures of disc degeneration, Humanin expression was downregulated.

    In plain wordsIn cells, natural Humanin levels were lower in disc degeneration models.

    Peptide
    Humanin
    Outcome
    DecreaseHN expression in nucleus pulposus cells
    Study

    humann: not reported in source

    human nucleus pulposus cell cultures
    Outcome group
    Aging markers · Musculoskeletal

    Source sentences

    Summary
    This pioneering study unveiled a downregulation of HN expression in IDD patients, a finding corroborated through cell and rat IDD models.
    1 context sentence
    Summary · context
    The expression profile of HN in IDD was scrutinized utilizing human NP cell cultures and an IDD rat model (n = 5).

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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