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Study · Ex vivo tissue · mouse · 2025

2 results

Nicotinamide mononucleotide restores impaired metabolism, endothelial cell proliferation and angiogenesis in old sedentary male mice.

Kiesworo K, Agius T, Macarthur MR, Lambelet M, Lyon A, Zhang J, Turiel G, Fan Z, d'Almeida S, Uygun K, Yeh H, Déglise S and 5 more

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de Bock K, Mitchell SJ, Ocampo A, Allagnat F, Longchamp A

iScience20 Dec 2024Cited by 10 (Europe PMC)

PMID39868046 (opens in a new tab)PMCIDPMC11763620 (opens in a new tab)DOI10.1016/j.isci.2024.111656 (opens in a new tab)PepBankPUB-39868046
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Summary

Aging is accompanied by a decline in neovascularization potential and increased susceptibility to ischemic injury. Here, we confirm the age-related impaired neovascularization following ischemic leg injury and impaired angiogenesis. The age-related deficits in angiogenesis arose primarily from diminished EC proliferation capacity, but not migration or VEGF sensitivity. Aged EC harvested from the mouse skeletal muscle displayed a pro-angiogenic gene expression phenotype, along with considerable changes in metabolic genes. Metabolomics analysis and 13C glucose tracing revealed impaired ATP production and blockade in glycolysis and TCA cycle in late passage HUVECs, which occurred at nicotinamide adenine dinucleotide (NAD⁺)-dependent steps, along with NAD+ depletion. Supplementation with nicotinamide mononucleotide (NMN), a precursor of NAD⁺, enhances late-passage EC proliferation and sprouting angiogenesis from aged mice aortas. Taken together, our study illustrates the importance of NAD+-dependent metabolism in the maintenance of EC proliferation capacity with age, and the therapeutic potential of NAD precursors.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueEfficacy outcome

    In aortas from aged mice, NMN enhanced sprouting angiogenesis.

    In plain wordsIn lab tests of aortas from aged mice, NMN increased blood vessel sprouting.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseSprouting angiogenesis
    Study

    mousen: not reported in source

    Aortas from aged mice
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Supplementation with nicotinamide mononucleotide (NMN), a precursor of NAD⁺, enhances late-passage EC proliferation and sprouting angiogenesis from aged mice aortas.
  2. 02LabIn vitro (cells)Efficacy outcome

    In late-passage endothelial cells, NMN enhanced proliferation.

    In plain wordsIn cells, NMN increased multiplication of late-passage endothelial cells.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseEndothelial cell proliferation
    Study

    n: not reported in source

    Late-passage endothelial cells
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Supplementation with nicotinamide mononucleotide (NMN), a precursor of NAD⁺, enhances late-passage EC proliferation and sprouting angiogenesis from aged mice aortas.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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