Thymosin β4 promotes zebrafish Mauthner axon regeneration by facilitating actin polymerization through binding to G-actin.

Song Z, Han A, Hu B

BMC biology23 Oct 2024Cited by 3 (Europe PMC)

PMID39443925PMCIDPMC11515629DOI10.1186/s12915-024-02045-2PepBankPUB-39443925

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Summary

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Text rights: CC BY-NC-ND. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone5

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

01AnimalNot a test of Thymosin beta-4 (human) aloneAnimal study (in vivo)Efficacy outcome
In zebrafish larvae, knockout of Tβ4 impaired axon regeneration.
In plain wordsIn zebrafish, removing Tβ4 worsened nerve fibre regrowth after injury.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseAxon regeneration after Tβ4 knockout
Study
zebrafishn: not reported in source
Zebrafish larvae Mauthner cell axon injury
Comparator
Wild type
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
knockout of Tβ4 impaired axon regeneration, whereas overexpression of Tβ4 promoted axon regeneration.
1 context sentence(design, population, supporting numbers)
Summary · context
Therefore, we used a single axon injury model of Mauthner cells in zebrafish larvae to investigate the role of Tβ4 in central axon regeneration in vivo.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-3944392501
02AnimalNot a test of Thymosin beta-4 (human) aloneAnimal study (in vivo)Efficacy outcome
In zebrafish larvae, overexpression of Tβ4 promoted axon regeneration.
In plain wordsIn zebrafish, extra Tβ4 improved nerve fibre regrowth after injury.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseAxon regeneration after Tβ4 overexpression
Study
zebrafishn: not reported in source
Zebrafish larvae Mauthner cell axon injury
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
overexpression of Tβ4 promoted axon regeneration.
1 context sentence(design, population, supporting numbers)
Summary · context
Therefore, we used a single axon injury model of Mauthner cells in zebrafish larvae to investigate the role of Tβ4 in central axon regeneration in vivo.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-3944392502
03AnimalNot a test of Thymosin beta-4 (human) aloneAnimal study (in vivo)Mechanism
In zebrafish larvae, the promotion of axon regeneration by Tβ4 was mediated through the interaction between Tβ4 and G-actin.
In plain wordsIn zebrafish, Tβ4 aided nerve regrowth by binding to G-actin.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableG-actin binding mediating axon regeneration
Study
zebrafishn: not reported in source
Zebrafish larvae Mauthner cell axon injury
Outcome group
Receptor activity and mechanism · Nervous system and cognition
Source sentences
Summary
Moreover, this promotion is mediated through the interaction between Tβ4 and G-actin.
1 context sentence(design, population, supporting numbers)
Summary · context
Therefore, we used a single axon injury model of Mauthner cells in zebrafish larvae to investigate the role of Tβ4 in central axon regeneration in vivo.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-3944392503
04AnimalNot a test of Thymosin beta-4 (human) aloneAnimal study (in vivo)Mechanism
In zebrafish larvae, binding of Tβ4 to G-actin promoted actin polymerization rather than depolymerization.
In plain wordsIn zebrafish, Tβ4 binding to G-actin promoted actin build-up rather than breakdown.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseActin polymerization
Study
zebrafishn: not reported in source
Zebrafish larvae Mauthner cell axon injury
Outcome group
Receptor activity and mechanism · Nervous system and cognition
Source sentences
Summary
Furthermore, our results suggest that the binding of Tβ4 to G-actin promotes actin polymerization rather than depolymerization.
1 context sentence(design, population, supporting numbers)
Summary · context
Therefore, we used a single axon injury model of Mauthner cells in zebrafish larvae to investigate the role of Tβ4 in central axon regeneration in vivo.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-3944392504
05AnimalNot a test of Thymosin beta-4 (human) aloneAnimal study (in vivo)Efficacy outcome
In zebrafish larvae, overexpression of Tβ4 effectively restored Mauthner cell-mediated rapid escape behavior.
In plain wordsIn zebrafish, extra Tβ4 restored the fish's quick escape movement after nerve injury.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseRapid escape behavior function
Study
zebrafishn: not reported in source
Zebrafish larvae Mauthner cell axon injury
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
Finally, the results showed that the overexpression of Tβ4 effectively restored the functionality of rapid escape behaviors mediated by Mauthner cells.
1 context sentence(design, population, supporting numbers)
Summary · context
Therefore, we used a single axon injury model of Mauthner cells in zebrafish larvae to investigate the role of Tβ4 in central axon regeneration in vivo.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-3944392505

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 01:52 UTC
Study results updated
Version 1
12 Oct 2026, 18:17 UTC
First added · source update from Europe PMC

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