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Study · Animal study (in vivo) · mouse · 2025

14 results

Oligoasthenozoospermia is alleviated in a mouse model by [Gly14]-humanin-mediated attenuation of oxidative stress and ferroptosis.

Liu Y, Feng Q, Zou L, Zhu C, Xia W

Andrology22 Oct 2024Cited by 4 (Europe PMC)

PMID39435863 (opens in a new tab)DOI10.1111/andr.13786 (opens in a new tab)PepBankPUB-39435863
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study14

13 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In mice with cyclophosphamide-induced oligoasthenozoospermia, [Gly14]-humanin improved testis and sperm parameters.

    In plain wordsIn mice with low sperm counts, [Gly14]-humanin improved testis and sperm measures compared with saline.

    Peptide
    Humanin
    Outcome
    IncreaseTestis and sperm parameters
    Study

    mousen: not reported in source

    Cyclophosphamide-induced oligoasthenozoospermia mice
    Comparator
    Saline-treated model mice
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The HNG treatment improved testis and sperm parameters
    2 context sentences
    Summary · context
    Mice were treated with cyclophosphamide (CP) to construct a mice model of oligoasthenozoospermia.
    Summary · context
    The resulting model mice were treated with saline or HNG.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In mice with cyclophosphamide-induced oligoasthenozoospermia, [Gly14]-humanin increased litter size.

    In plain wordsIn mice with low sperm counts, [Gly14]-humanin increased litter size compared with saline.

    Peptide
    Humanin
    Outcome
    IncreaseLitter size
    Study

    mousen: not reported in source

    Cyclophosphamide-induced oligoasthenozoospermia mice
    Comparator
    Saline-treated model mice
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The HNG treatment improved testis and sperm parameters and increased litter size and serum testosterone concentrations in model mice.
    2 context sentences
    Summary · context
    Mice were treated with cyclophosphamide (CP) to construct a mice model of oligoasthenozoospermia.
    Summary · context
    The resulting model mice were treated with saline or HNG.
  3. 03AnimalAnimal study (in vivo)Efficacy outcome

    In mice with cyclophosphamide-induced oligoasthenozoospermia, [Gly14]-humanin increased serum testosterone concentrations.

    In plain wordsIn mice with low sperm counts, [Gly14]-humanin raised blood testosterone compared with saline.

    Peptide
    Humanin
    Outcome
    IncreaseSerum testosterone
    Study

    mousen: not reported in source

    Cyclophosphamide-induced oligoasthenozoospermia mice
    Comparator
    Saline-treated model mice
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The HNG treatment improved testis and sperm parameters and increased litter size and serum testosterone concentrations in model mice.
    2 context sentences
    Summary · context
    Mice were treated with cyclophosphamide (CP) to construct a mice model of oligoasthenozoospermia.
    Summary · context
    The resulting model mice were treated with saline or HNG.
  4. 04AnimalAnimal study (in vivo)Mechanism

    In mice with cyclophosphamide-induced oligoasthenozoospermia, [Gly14]-humanin increased expression of ferroptosis-related proteins in testicular tissue.

    In plain wordsIn mice with low sperm counts, [Gly14]-humanin increased levels of ferroptosis-related proteins in testis tissue.

    Peptide
    Humanin
    Outcome
    IncreaseFerroptosis-related protein expression in testis
    Study

    mousen: not reported in source

    Cyclophosphamide-induced oligoasthenozoospermia mice
    Comparator
    Saline-treated model mice
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The expression of ferroptosis-related proteins increased in testicular tissues after the HNG treatment.
    2 context sentences
    Summary · context
    Mice were treated with cyclophosphamide (CP) to construct a mice model of oligoasthenozoospermia.
    Summary · context
    The resulting model mice were treated with saline or HNG.
  5. 05AnimalAnimal study (in vivo)Mechanism

    In mice with cyclophosphamide-induced oligoasthenozoospermia, [Gly14]-humanin decreased ROS concentrations in testicular tissue.

    In plain wordsIn mice with low sperm counts, [Gly14]-humanin lowered reactive oxygen species in testis tissue.

    Peptide
    Humanin
    Outcome
    DecreaseROS in testicular tissue
    Study

    mousen: not reported in source

    Cyclophosphamide-induced oligoasthenozoospermia mice
    Comparator
    Saline-treated model mice
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The concentrations of ROS, MDA, and Fe2+ decreased and the concentrations of GSH increased in testicular tissues and in TM3 and TM4 cells after HNG treatment.
    2 context sentences
    Summary · context
    Mice were treated with cyclophosphamide (CP) to construct a mice model of oligoasthenozoospermia.
    Summary · context
    The resulting model mice were treated with saline or HNG.
  6. 06AnimalAnimal study (in vivo)Mechanism

    In mice with cyclophosphamide-induced oligoasthenozoospermia, [Gly14]-humanin decreased malondialdehyde concentrations in testicular tissue.

    In plain wordsIn mice with low sperm counts, [Gly14]-humanin lowered malondialdehyde, an oxidative damage marker, in testis tissue.

    Peptide
    Humanin
    Outcome
    DecreaseMDA in testicular tissue
    Study

    mousen: not reported in source

    Cyclophosphamide-induced oligoasthenozoospermia mice
    Comparator
    Saline-treated model mice
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The concentrations of ROS, MDA, and Fe2+ decreased and the concentrations of GSH increased in testicular tissues and in TM3 and TM4 cells after HNG treatment.
    2 context sentences
    Summary · context
    Mice were treated with cyclophosphamide (CP) to construct a mice model of oligoasthenozoospermia.
    Summary · context
    The resulting model mice were treated with saline or HNG.
  7. 07AnimalAnimal study (in vivo)Mechanism

    In mice with cyclophosphamide-induced oligoasthenozoospermia, [Gly14]-humanin decreased ferrous ion concentrations in testicular tissue.

    In plain wordsIn mice with low sperm counts, [Gly14]-humanin lowered ferrous iron in testis tissue.

    Peptide
    Humanin
    Outcome
    DecreaseFe2+ in testicular tissue
    Study

    mousen: not reported in source

    Cyclophosphamide-induced oligoasthenozoospermia mice
    Comparator
    Saline-treated model mice
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The concentrations of ROS, MDA, and Fe2+ decreased and the concentrations of GSH increased in testicular tissues and in TM3 and TM4 cells after HNG treatment.
    2 context sentences
    Summary · context
    Mice were treated with cyclophosphamide (CP) to construct a mice model of oligoasthenozoospermia.
    Summary · context
    The resulting model mice were treated with saline or HNG.
  8. 08AnimalAnimal study (in vivo)Mechanism

    In mice with cyclophosphamide-induced oligoasthenozoospermia, [Gly14]-humanin increased glutathione concentrations in testicular tissue.

    In plain wordsIn mice with low sperm counts, [Gly14]-humanin raised glutathione, an antioxidant, in testis tissue.

    Peptide
    Humanin
    Outcome
    IncreaseGSH in testicular tissue
    Study

    mousen: not reported in source

    Cyclophosphamide-induced oligoasthenozoospermia mice
    Comparator
    Saline-treated model mice
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The concentrations of ROS, MDA, and Fe2+ decreased and the concentrations of GSH increased in testicular tissues and in TM3 and TM4 cells after HNG treatment.
    2 context sentences
    Summary · context
    Mice were treated with cyclophosphamide (CP) to construct a mice model of oligoasthenozoospermia.
    Summary · context
    The resulting model mice were treated with saline or HNG.
  9. 09LabIn vitro (cells)Mechanism

    In TM3 and TM4 cells with erastin-induced ferroptosis, [Gly14]-humanin decreased ROS concentrations.

    In plain wordsIn lab-grown testis cells under ferroptosis stress, [Gly14]-humanin lowered reactive oxygen species.

    Peptide
    Humanin
    Outcome
    DecreaseROS in TM3 and TM4 cells
    Study

    n: not reported in source

    TM3 and TM4 cells with erastin-induced ferroptosis
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The concentrations of ROS, MDA, and Fe2+ decreased and the concentrations of GSH increased in testicular tissues and in TM3 and TM4 cells after HNG treatment.
    1 context sentence
    Summary · context
    TM3, TM4, GC1, and GC2 cells were exposed to erastin to induce ferroptosis, followed by treatment with HNG or HNG + ML385 (a nuclear factor erythroid 2-related factor 2 inhibitor).
  10. 10LabIn vitro (cells)Mechanism

    In TM3 and TM4 cells with erastin-induced ferroptosis, [Gly14]-humanin decreased malondialdehyde concentrations.

    In plain wordsIn lab-grown testis cells under ferroptosis stress, [Gly14]-humanin lowered malondialdehyde, an oxidative damage marker.

    Peptide
    Humanin
    Outcome
    DecreaseMDA in TM3 and TM4 cells
    Study

    n: not reported in source

    TM3 and TM4 cells with erastin-induced ferroptosis
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The concentrations of ROS, MDA, and Fe2+ decreased and the concentrations of GSH increased in testicular tissues and in TM3 and TM4 cells after HNG treatment.
    1 context sentence
    Summary · context
    TM3, TM4, GC1, and GC2 cells were exposed to erastin to induce ferroptosis, followed by treatment with HNG or HNG + ML385 (a nuclear factor erythroid 2-related factor 2 inhibitor).
  11. 11LabIn vitro (cells)Mechanism

    In TM3 and TM4 cells with erastin-induced ferroptosis, [Gly14]-humanin decreased ferrous ion concentrations.

    In plain wordsIn lab-grown testis cells under ferroptosis stress, [Gly14]-humanin lowered ferrous iron.

    Peptide
    Humanin
    Outcome
    DecreaseFe2+ in TM3 and TM4 cells
    Study

    n: not reported in source

    TM3 and TM4 cells with erastin-induced ferroptosis
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The concentrations of ROS, MDA, and Fe2+ decreased and the concentrations of GSH increased in testicular tissues and in TM3 and TM4 cells after HNG treatment.
    1 context sentence
    Summary · context
    TM3, TM4, GC1, and GC2 cells were exposed to erastin to induce ferroptosis, followed by treatment with HNG or HNG + ML385 (a nuclear factor erythroid 2-related factor 2 inhibitor).
  12. 12LabIn vitro (cells)Mechanism

    In TM3 and TM4 cells with erastin-induced ferroptosis, [Gly14]-humanin increased glutathione concentrations.

    In plain wordsIn lab-grown testis cells under ferroptosis stress, [Gly14]-humanin raised glutathione, an antioxidant.

    Peptide
    Humanin
    Outcome
    IncreaseGSH in TM3 and TM4 cells
    Study

    n: not reported in source

    TM3 and TM4 cells with erastin-induced ferroptosis
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The concentrations of ROS, MDA, and Fe2+ decreased and the concentrations of GSH increased in testicular tissues and in TM3 and TM4 cells after HNG treatment.
    1 context sentence
    Summary · context
    TM3, TM4, GC1, and GC2 cells were exposed to erastin to induce ferroptosis, followed by treatment with HNG or HNG + ML385 (a nuclear factor erythroid 2-related factor 2 inhibitor).
  13. 13LabIn vitro (cells)Mechanism

    In cells with erastin-induced ferroptosis, [Gly14]-humanin activated the Nrf2/GPX4 pathway.

    In plain wordsIn lab-grown testis cells under ferroptosis stress, [Gly14]-humanin switched on the Nrf2/GPX4 protection pathway.

    Peptide
    Humanin
    Outcome
    IncreaseNrf2/GPX4 pathway activation
    Study

    n: not reported in source

    TM3 and TM4 cells with erastin-induced ferroptosis
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In vitro experiments confirmed that HNG activated the nuclear factor erythroid 2-related factor 2/glutathione peroxidase 4 (Nrf2/GPX4) pathway.
    1 context sentence
    Summary · context
    TM3, TM4, GC1, and GC2 cells were exposed to erastin to induce ferroptosis, followed by treatment with HNG or HNG + ML385 (a nuclear factor erythroid 2-related factor 2 inhibitor).

Studies that did not test the peptide alone1

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

  1. 14LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    In cells with erastin-induced ferroptosis, the effects of [Gly14]-humanin were blocked by the Nrf2 inhibitor ML385.

    In plain wordsIn lab-grown testis cells, blocking the Nrf2 protein with ML385 stopped the protective effects of [Gly14]-humanin.

    Peptide
    Humanin
    Outcome
    Not applicableHNG effects with the Nrf2 inhibitor ML385
    Study

    n: not reported in source

    TM3 and TM4 cells with erastin-induced ferroptosis
    Comparator
    HNG alone
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health

    Source sentences

    Summary
    However, these effects of HNG were blocked by ML385 treatment.
    1 context sentence
    Summary · context
    TM3, TM4, GC1, and GC2 cells were exposed to erastin to induce ferroptosis, followed by treatment with HNG or HNG + ML385 (a nuclear factor erythroid 2-related factor 2 inhibitor).

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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