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Study · Ex vivo tissue · 2024

4 results

Mitochondrial-derived peptides, HNG and SHLP3, protect cochlear hair cells against gentamicin.

Lu Y, Bartoszek EM, Cortada M, Bodmer D, Levano Huaman S

Cell death discovery21 Oct 2024Cited by 7 (Europe PMC)

PMID39433756 (opens in a new tab)PMCIDPMC11493991 (opens in a new tab)DOI10.1038/s41420-024-02215-9 (opens in a new tab)PepBankPUB-39433756
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Summary

Preservation of hair cells is critical for maintaining hearing function, as damage to sensory cells potentially leads to irreparable sensorineural hearing loss. Hair cell loss is often associated with inflammation and oxidative stress. One promising class of bioactive peptides is mitochondrial-derived peptides (MDPs), which have already been proven to protect various tissues from cellular stresses and delay aging processes. Humanin (HN) is one of the best-known members of this family, and recently, we have shown its protective effect in hair cells. The synthetic derivate HN S14G (HNG) has a more potent protective effect than natural HN making it a more useful peptide candidate to promote cytoprotection. A less-known MDP is small humanin-like peptide 3 (SHLP3), which has cytoprotective effects similar to HN, but likely acts through different signaling pathways. Therefore, we examined the effect of exogenous HNG and SHLP3 in auditory hair cells and investigated the molecular mechanisms involved. For this purpose, explants of the organ of Corti (OC) were treated with gentamicin in the presence and absence of HNG or SHLP3. Administration of HNG and SHLP3 reduced gentamicin-induced hair cell loss. The protective mechanisms of HNG and SHLP3 in OC explants included, in part, modulation of AKT and AMPKα. In addition, treatment with HNG and SHLP3 reduced gentamicin-induced oxidative stress and inflammatory gene overexpression. Overall, our data show that HNG and SHLP3 protect hair cells from gentamicin-induced toxicity. This offers new perspectives for the development of therapeutic strategies with MDPs against hearing loss.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueEfficacy outcome

    In organ of Corti explants treated with gentamicin, HNG reduced hair cell loss.

    In plain wordsIn lab-grown ear tissue exposed to gentamicin, HNG reduced the loss of hair cells.

    Peptide
    Humanin
    Outcome
    DecreaseGentamicin-induced hair cell loss
    Study

    n: not reported in source

    Organ of Corti explants with gentamicin
    Comparator
    Gentamicin without HNG
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Administration of HNG and SHLP3 reduced gentamicin-induced hair cell loss.
    1 context sentence
    Summary · context
    For this purpose, explants of the organ of Corti (OC) were treated with gentamicin in the presence and absence of HNG or SHLP3.
  2. 02LabEx vivo tissueMechanism

    In organ of Corti explants, the protective mechanisms of HNG included, in part, modulation of AKT and AMPKα.

    In plain wordsIn lab-grown ear tissue, HNG protected hair cells partly by changing the activity of AKT and AMPKα.

    Peptide
    Humanin
    Outcome
    MixedModulation of AKT and AMPKα
    Study

    n: not reported in source

    Organ of Corti explants with gentamicin
    Comparator
    Gentamicin without HNG
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The protective mechanisms of HNG and SHLP3 in OC explants included, in part, modulation of AKT and AMPKα.
    1 context sentence
    Summary · context
    For this purpose, explants of the organ of Corti (OC) were treated with gentamicin in the presence and absence of HNG or SHLP3.
  3. 03LabEx vivo tissueMechanism

    In organ of Corti explants, HNG treatment reduced gentamicin-induced oxidative stress.

    In plain wordsIn lab-grown ear tissue, HNG reduced oxidative stress caused by gentamicin.

    Peptide
    Humanin
    Outcome
    DecreaseGentamicin-induced oxidative stress
    Study

    n: not reported in source

    Organ of Corti explants with gentamicin
    Comparator
    Gentamicin without HNG
    Outcome group
    Inflammation markers · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In addition, treatment with HNG and SHLP3 reduced gentamicin-induced oxidative stress and inflammatory gene overexpression.
    1 context sentence
    Summary · context
    For this purpose, explants of the organ of Corti (OC) were treated with gentamicin in the presence and absence of HNG or SHLP3.
  4. 04LabEx vivo tissueMechanism

    In organ of Corti explants, HNG treatment reduced gentamicin-induced overexpression of inflammatory genes.

    In plain wordsIn lab-grown ear tissue, HNG reduced the switching on of inflammation genes caused by gentamicin.

    Peptide
    Humanin
    Outcome
    DecreaseGentamicin-induced inflammatory gene overexpression
    Study

    n: not reported in source

    Organ of Corti explants with gentamicin
    Comparator
    Gentamicin without HNG
    Outcome group
    Inflammation markers · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In addition, treatment with HNG and SHLP3 reduced gentamicin-induced oxidative stress and inflammatory gene overexpression.
    1 context sentence
    Summary · context
    For this purpose, explants of the organ of Corti (OC) were treated with gentamicin in the presence and absence of HNG or SHLP3.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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