Metabolomics analysis of the effect of GnRH on the pregnancy rate of ewes with estrus synchronization scheme based on progesterone.

Zhang J, Sun S, Bai X, Yang N, Liu Y, Wu X, Li X

Frontiers in veterinary science11 Jul 2024Cited by 4 (Europe PMC)

PMID39055862PMCIDPMC11270128DOI10.3389/fvets.2024.1442931PepBankPUB-39055862

Summary

IntroductionGonadotropin-releasing hormone (GnRH) is widely used in the timed artificial insemination protocol for sheep. However, there remains a debate regarding its impact on pregnancy rates during artificial insemination. This study aims to evaluate the effect of GnRH on the pregnancy rates in Huyang ewes, analyze the pre-implantation metabolite changes caused by GnRH using metabolomics, and elucidate the mechanism effect on pregnancy rates.MethodsAll ewes were administered a vaginal progesterone sponge containing 45 mg of flurogestone acetate for 12 days and received 330 units of equine chorionic gonadotropin (eCG) intramuscularly after sponge removal. The experimental group (n = 69) received an intramuscular treatment of 17 μg GnRH agonist triptorelin 48 h after sponge removal on Day 0, while the control group (n = 41) received 1 mL of sterile saline solution. All ewes underwent a single vaginal insemination 58 h after the withdrawal of the progesterone sponge. The difference in pregnancy rates between the two groups was calculated. Metabolomic analysis was performed on plasma samples collected on Day 7 after the treatment of GnRH agonist.ResultsGonadotropin-releasing hormone (GnRH) treatment significantly reduced the pregnancy rate in the experimental group compared with the control group (72.2 vs. 82.9%, p < 0.05). Metabolomic analysis indicated that GnRH treatment affected metabolites involved in collagen synthesis and prostaglandin synthesis in the endometrial tissue, which includes a marked decrease in hydroxyproline amino acid content and a significant increase in corticosterone and prostaglandin D2 lipids and unsaturated fatty acids.ConclusionIn summary, the injection of GnRH agonist Triptorelin 48 h after progesterone sponges removal reduces the pregnancy rate of Huyang ewe following artificial insemination. It also affects the metabolite levels related to endometrial collagen and prostaglandin synthesis, harming embryo implantation.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In Huyang ewes, triptorelin reduced pregnancy rates versus saline after estrus synchronization and artificial insemination.
In plain wordsIn sheep, triptorelin reduced pregnancy rates compared with saline after estrus synchronization and artificial insemination.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
DecreasePregnancy rate
Study
sheepn = 110
Huyang ewes undergoing estrus synchronization and artificial insemination
Comparator
Saline
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
17 μg · intramuscularAs given in this study (sheep).
Measurements
Pregnancy rate
72.2%
triptorelin
Pregnancy rate
82.9%
control
Source sentences
Summary
Gonadotropin-releasing hormone (GnRH) treatment significantly reduced the pregnancy rate in the experimental group compared with the control group (72.2 vs. 82.9%, p < 0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
All ewes were administered a vaginal progesterone sponge containing 45 mg of flurogestone acetate for 12 days and received 330 units of equine chorionic gonadotropin (eCG) intramuscularly after sponge removal.
Summary · context
The experimental group (n = 69) received an intramuscular treatment of 17 μg GnRH agonist triptorelin 48 h after sponge removal on Day 0, while the control group (n = 41) received 1 mL of sterile saline solution.
Show in the study text Report a problemFND-3905586201
02AnimalAnimal study (in vivo)Mechanism
In Huyang ewes, triptorelin reduced hydroxyproline content compared with controls.
In plain wordsIn sheep, triptorelin reduced hydroxyproline content compared with controls.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
DecreaseHydroxyproline content
Study
sheepn: not reported in source
Huyang ewes undergoing estrus synchronization and artificial insemination
Comparator
Saline
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Studied dose
17 μg · intramuscularAs given in this study (sheep).
Source sentences
Summary
Metabolomic analysis indicated that GnRH treatment affected metabolites involved in collagen synthesis and prostaglandin synthesis in the endometrial tissue, which includes a marked decrease in hydroxyproline amino acid content and a significant increase in corticosterone and prostaglandin D2 lipids and unsaturated fatty acids.
2 context sentences(design, population, supporting numbers)
Summary · context
All ewes were administered a vaginal progesterone sponge containing 45 mg of flurogestone acetate for 12 days and received 330 units of equine chorionic gonadotropin (eCG) intramuscularly after sponge removal.
Summary · context
The experimental group (n = 69) received an intramuscular treatment of 17 μg GnRH agonist triptorelin 48 h after sponge removal on Day 0, while the control group (n = 41) received 1 mL of sterile saline solution.
Show in the study text Report a problemFND-3905586202
03AnimalAnimal study (in vivo)Mechanism
In Huyang ewes, triptorelin increased corticosterone compared with controls.
In plain wordsIn sheep, triptorelin increased corticosterone compared with controls.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
IncreaseCorticosterone
Study
sheepn: not reported in source
Huyang ewes undergoing estrus synchronization and artificial insemination
Comparator
Saline
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
17 μg · intramuscularAs given in this study (sheep).
Source sentences
Summary
Metabolomic analysis indicated that GnRH treatment affected metabolites involved in collagen synthesis and prostaglandin synthesis in the endometrial tissue, which includes a marked decrease in hydroxyproline amino acid content and a significant increase in corticosterone and prostaglandin D2 lipids and unsaturated fatty acids.
2 context sentences(design, population, supporting numbers)
Summary · context
All ewes were administered a vaginal progesterone sponge containing 45 mg of flurogestone acetate for 12 days and received 330 units of equine chorionic gonadotropin (eCG) intramuscularly after sponge removal.
Summary · context
The experimental group (n = 69) received an intramuscular treatment of 17 μg GnRH agonist triptorelin 48 h after sponge removal on Day 0, while the control group (n = 41) received 1 mL of sterile saline solution.
Show in the study text Report a problemFND-3905586203
04AnimalAnimal study (in vivo)Mechanism
In Huyang ewes, triptorelin increased prostaglandin D2 compared with controls.
In plain wordsIn sheep, triptorelin increased prostaglandin D2 compared with controls.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
IncreaseProstaglandin D2
Study
sheepn: not reported in source
Huyang ewes undergoing estrus synchronization and artificial insemination
Comparator
Saline
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Studied dose
17 μg · intramuscularAs given in this study (sheep).
Source sentences
Summary
Metabolomic analysis indicated that GnRH treatment affected metabolites involved in collagen synthesis and prostaglandin synthesis in the endometrial tissue, which includes a marked decrease in hydroxyproline amino acid content and a significant increase in corticosterone and prostaglandin D2 lipids and unsaturated fatty acids.
2 context sentences(design, population, supporting numbers)
Summary · context
All ewes were administered a vaginal progesterone sponge containing 45 mg of flurogestone acetate for 12 days and received 330 units of equine chorionic gonadotropin (eCG) intramuscularly after sponge removal.
Summary · context
The experimental group (n = 69) received an intramuscular treatment of 17 μg GnRH agonist triptorelin 48 h after sponge removal on Day 0, while the control group (n = 41) received 1 mL of sterile saline solution.
Show in the study text Report a problemFND-3905586204
05AnimalAnimal study (in vivo)Mechanism
In Huyang ewes, triptorelin increased unsaturated fatty acids compared with controls.
In plain wordsIn sheep, triptorelin increased unsaturated fatty acids compared with controls.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
IncreaseUnsaturated fatty acids
Study
sheepn: not reported in source
Huyang ewes undergoing estrus synchronization and artificial insemination
Comparator
Saline
Outcome group
Lipids · Metabolic and weight
Studied dose
17 μg · intramuscularAs given in this study (sheep).
Source sentences
Summary
Metabolomic analysis indicated that GnRH treatment affected metabolites involved in collagen synthesis and prostaglandin synthesis in the endometrial tissue, which includes a marked decrease in hydroxyproline amino acid content and a significant increase in corticosterone and prostaglandin D2 lipids and unsaturated fatty acids.
2 context sentences(design, population, supporting numbers)
Summary · context
All ewes were administered a vaginal progesterone sponge containing 45 mg of flurogestone acetate for 12 days and received 330 units of equine chorionic gonadotropin (eCG) intramuscularly after sponge removal.
Summary · context
The experimental group (n = 69) received an intramuscular treatment of 17 μg GnRH agonist triptorelin 48 h after sponge removal on Day 0, while the control group (n = 41) received 1 mL of sterile saline solution.
Show in the study text Report a problemFND-3905586205

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PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
22 Oct 2026, 02:26 UTC
Study results updated
Version 1
21 Oct 2026, 18:59 UTC
First added · source update from Europe PMC

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