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Study · In vitro (cells) · wistar rat · 2024

8 results

Melanocortin-receptor 4 activation modulates proliferation and differentiation of rat postnatal hippocampal neural precursor cells.

Carniglia L, Turati J, Saba J, López Couselo F, Romero AC, Caruso C, Durand D, Lasaga M

Neuropharmacology1 Jul 2024Cited by 2 (Europe PMC)

PMID38960135 (opens in a new tab)DOI10.1016/j.neuropharm.2024.110058 (opens in a new tab)PepBankPUB-38960135
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

8 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In growth-factor-deprived rat postnatal hippocampal neural precursor cells, 10 nM NDP-MSH for 6 days increased the proportion of Ki-67-positive cells compared with control.

    In plain wordsIn rat brain precursor cells in a dish, NDP-MSH increased the share of dividing cells compared with control.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseKi-67-positive cell proportion
    Study

    Wistar ratn: not reported in source

    Growth-factor-deprived postnatal hippocampal neural precursor cells
    Comparator
    Control
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    10 nM · incubation · 6 daysAs given in this study (Wistar rat).

    Source sentences

    Summary
    Incubation of growth-factor deprived NPCs with 10 nM NDP-MSH for 6 days increased the proportion of Ki-67- and 5-bromo-2'-deoxyuridine (BrdU)-positive cells, compared to the control group
    1 context sentence
    Summary · context
    Here, we used postnatally-isolated hippocampal NPCs from Wistar rat pups (male and female combined) to determine the role of the melanocortin analog [Nle4, D-Phe7]-α-MSH (NDP-MSH) in proliferation and fate acquisition of NPCs.
  2. 02LabIn vitro (cells)Efficacy outcome

    In growth-factor-deprived rat postnatal hippocampal neural precursor cells, 10 nM NDP-MSH for 6 days increased the proportion of BrdU-positive cells compared with control.

    In plain wordsIn rat brain precursor cells in a dish, NDP-MSH increased the share of cells that copied their DNA compared with control.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseBrdU-positive cell proportion
    Study

    Wistar ratn: not reported in source

    Growth-factor-deprived postnatal hippocampal neural precursor cells
    Comparator
    Control
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    10 nM · incubation · 6 daysAs given in this study (Wistar rat).

    Source sentences

    Summary
    Incubation of growth-factor deprived NPCs with 10 nM NDP-MSH for 6 days increased the proportion of Ki-67- and 5-bromo-2'-deoxyuridine (BrdU)-positive cells, compared to the control group
    1 context sentence
    Summary · context
    Here, we used postnatally-isolated hippocampal NPCs from Wistar rat pups (male and female combined) to determine the role of the melanocortin analog [Nle4, D-Phe7]-α-MSH (NDP-MSH) in proliferation and fate acquisition of NPCs.
  3. 03LabIn vitro (cells)Mechanism

    In rat hippocampal neural precursor cells, the MC4R antagonist JKC-363 blocked the increase in Ki-67- and BrdU-positive cells caused by NDP-MSH.

    In plain wordsIn rat brain precursor cells in a dish, a drug that blocks the MC4R receptor stopped the NDP-MSH effect on cell division.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    DecreaseMC4R antagonist JKC-363 block of NDP-MSH proliferation effect
    Study

    Wistar ratn: not reported in source

    Growth-factor-deprived postnatal hippocampal neural precursor cells
    Comparator
    NDP-MSH without antagonist
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    10 nM · incubation · 6 daysAs given in this study (Wistar rat).

    Source sentences

    Summary
    these effects were blocked by the MC4R antagonist JKC-363.
    1 context sentence
    Summary · context
    Here, we used postnatally-isolated hippocampal NPCs from Wistar rat pups (male and female combined) to determine the role of the melanocortin analog [Nle4, D-Phe7]-α-MSH (NDP-MSH) in proliferation and fate acquisition of NPCs.
  4. 04LabIn vitro (cells)Efficacy outcome

    In rat hippocampal neural precursor cells, NDP-MSH increased the proportion of GFAP/Ki-67 double-positive cells.

    In plain wordsIn rat brain precursor cells in a dish, NDP-MSH increased the share of dividing type-1 and type-2 precursor cells.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseGFAP/Ki-67 double-positive cell proportion
    Study

    Wistar ratn: not reported in source

    Growth-factor-deprived postnatal hippocampal neural precursor cells
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    NDP-MSH also increased the proportion of glial fibrillar acidic protein (GFAP)/Ki-67, GFAP/sex-determining region Y-box2 (SOX2) and neuroepithelial stem cell protein (NESTIN)/Ki-67-double positive cells (type-1 and type-2 precursors).
    1 context sentence
    Summary · context
    Here, we used postnatally-isolated hippocampal NPCs from Wistar rat pups (male and female combined) to determine the role of the melanocortin analog [Nle4, D-Phe7]-α-MSH (NDP-MSH) in proliferation and fate acquisition of NPCs.
  5. 05LabIn vitro (cells)Efficacy outcome

    In rat hippocampal neural precursor cells, NDP-MSH increased the proportion of GFAP/SOX2 double-positive cells.

    In plain wordsIn rat brain precursor cells in a dish, NDP-MSH increased the share of GFAP/SOX2 precursor cells.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseGFAP/SOX2 double-positive cell proportion
    Study

    Wistar ratn: not reported in source

    Growth-factor-deprived postnatal hippocampal neural precursor cells
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    NDP-MSH also increased the proportion of glial fibrillar acidic protein (GFAP)/Ki-67, GFAP/sex-determining region Y-box2 (SOX2) and neuroepithelial stem cell protein (NESTIN)/Ki-67-double positive cells (type-1 and type-2 precursors).
    1 context sentence
    Summary · context
    Here, we used postnatally-isolated hippocampal NPCs from Wistar rat pups (male and female combined) to determine the role of the melanocortin analog [Nle4, D-Phe7]-α-MSH (NDP-MSH) in proliferation and fate acquisition of NPCs.
  6. 06LabIn vitro (cells)Efficacy outcome

    In rat hippocampal neural precursor cells, NDP-MSH increased the proportion of NESTIN/Ki-67 double-positive cells.

    In plain wordsIn rat brain precursor cells in a dish, NDP-MSH increased the share of dividing NESTIN-positive precursor cells.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseNESTIN/Ki-67 double-positive cell proportion
    Study

    Wistar ratn: not reported in source

    Growth-factor-deprived postnatal hippocampal neural precursor cells
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    NDP-MSH also increased the proportion of glial fibrillar acidic protein (GFAP)/Ki-67, GFAP/sex-determining region Y-box2 (SOX2) and neuroepithelial stem cell protein (NESTIN)/Ki-67-double positive cells (type-1 and type-2 precursors).
    1 context sentence
    Summary · context
    Here, we used postnatally-isolated hippocampal NPCs from Wistar rat pups (male and female combined) to determine the role of the melanocortin analog [Nle4, D-Phe7]-α-MSH (NDP-MSH) in proliferation and fate acquisition of NPCs.
  7. 07LabIn vitro (cells)Mechanism

    In rat hippocampal neural precursor cells, NDP-MSH induced PPAR-γ protein expression.

    In plain wordsIn rat brain precursor cells in a dish, NDP-MSH raised the level of the PPAR-γ protein.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreasePPAR-γ protein expression
    Study

    Wistar ratn: not reported in source

    Growth-factor-deprived postnatal hippocampal neural precursor cells
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    NDP-MSH induced peroxisome proliferator-activated receptor (PPAR)-γ protein expression
    1 context sentence
    Summary · context
    Here, we used postnatally-isolated hippocampal NPCs from Wistar rat pups (male and female combined) to determine the role of the melanocortin analog [Nle4, D-Phe7]-α-MSH (NDP-MSH) in proliferation and fate acquisition of NPCs.
  8. 08LabIn vitro (cells)Mechanism

    In rat hippocampal neural precursor cells, co-incubation with the PPAR-γ inhibitor GW9662 prevented the effect of NDP-MSH on proliferation and differentiation.

    In plain wordsIn rat brain precursor cells in a dish, blocking PPAR-γ stopped the NDP-MSH effect on cell growth and fate.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    DecreasePPAR-γ inhibitor GW9662 block of NDP-MSH effect on proliferation and differentiation
    Study

    Wistar ratn: not reported in source

    Growth-factor-deprived postnatal hippocampal neural precursor cells
    Comparator
    NDP-MSH without inhibitor
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    co-incubation with the PPAR-γ inhibitor GW9662 prevented the effect of NDP-MSH on NPC proliferation and differentiation
    1 context sentence
    Summary · context
    Here, we used postnatally-isolated hippocampal NPCs from Wistar rat pups (male and female combined) to determine the role of the melanocortin analog [Nle4, D-Phe7]-α-MSH (NDP-MSH) in proliferation and fate acquisition of NPCs.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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