Supplementing Boar Diet with Nicotinamide Mononucleotide Improves Sperm Quality Probably through the Activation of the SIRT3 Signaling Pathway.

Zhang H, Chai J, Cao C, Wang X, Pang W

Antioxidants (Basel, Switzerland)24 Apr 2024Cited by 14 (Europe PMC)

PMID38790612PMCIDPMC11117624DOI10.3390/antiox13050507PepBankPUB-38790612

Summary

Sperm quality is an important indicator to evaluate the reproduction ability of animals. Nicotinamide mononucleotide (NMN) participates in cell energy metabolism and reduces cell oxidative stress. However, the effect and regulatory mechanism of NMN on porcine sperm quality are still unknown. Here, 32 Landrace boars were randomly assigned to four groups (n = 8) and fed with different levels of NMN (0, 8, 16 or 32 mg/kg/d) for 9 weeks, and then serum and semen samples of the boars were collected to investigate the function and molecular mechanism of NMN in sperm quality. The results showed that the dietary NMN supplementation significantly increased sperm volume, density and motility (p < 0.05). Interestingly, NMN apparently improved the antioxidative indexes and increased the levels of testosterone (p < 0.05) in serum. Furthermore, NMN upregulated the protein levels of sirtuin 3 (SIRT3), antioxidation and oxidative phosphorylation (OXPHOS), but downregulated the protein levels of apoptosis in semen. Mechanically, NMN protected sperm from H₂O₂-induced oxidative stress and apoptosis through SIRT3 deacetylation. Importantly, the SIRT3-specific inhibitor 3-TYP attenuated the antioxidation and antiapoptosis of NMN in sperm. Therefore, NMN exerts antioxidation and antiapoptosis to improve boar sperm quality via the SIRT3 signaling pathway. Our findings suggest that NMN is a novel potential boar antioxidative feed additive to produce high-quality porcine semen.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study13

11 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Mechanism
In boars, dietary NMN increased sperm volume.
In plain wordsIn boars, dietary NMN increased sperm volume.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseSperm volume
Study
pign = 32
Landrace boars
Comparator
0 mg/kg/d NMN
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
8, 16 or 32 mg/kg/d · oral · 9 weeksAs given in this study (pig).
Source sentences
Summary
The results showed that the dietary NMN supplementation significantly increased sperm volume, density and motility (p < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
Here, 32 Landrace boars were randomly assigned to four groups (n = 8) and fed with different levels of NMN (0, 8, 16 or 32 mg/kg/d) for 9 weeks, and then serum and semen samples of the boars were collected to investigate the function and molecular mechanism of NMN in sperm quality.
Show in the study text Report a problemFND-3879061201
02AnimalAnimal study (in vivo)Mechanism
In boars, dietary NMN increased sperm density.
In plain wordsIn boars, dietary NMN increased sperm density.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseSperm density
Study
pign = 32
Landrace boars
Comparator
0 mg/kg/d NMN
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
8, 16 or 32 mg/kg/d · oral · 9 weeksAs given in this study (pig).
Source sentences
Summary
The results showed that the dietary NMN supplementation significantly increased sperm volume, density and motility (p < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
Here, 32 Landrace boars were randomly assigned to four groups (n = 8) and fed with different levels of NMN (0, 8, 16 or 32 mg/kg/d) for 9 weeks, and then serum and semen samples of the boars were collected to investigate the function and molecular mechanism of NMN in sperm quality.
Show in the study text Report a problemFND-3879061202
03AnimalAnimal study (in vivo)Mechanism
In boars, dietary NMN increased sperm motility.
In plain wordsIn boars, dietary NMN increased sperm movement.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseSperm motility
Study
pign = 32
Landrace boars
Comparator
0 mg/kg/d NMN
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
8, 16 or 32 mg/kg/d · oral · 9 weeksAs given in this study (pig).
Source sentences
Summary
The results showed that the dietary NMN supplementation significantly increased sperm volume, density and motility (p < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
Here, 32 Landrace boars were randomly assigned to four groups (n = 8) and fed with different levels of NMN (0, 8, 16 or 32 mg/kg/d) for 9 weeks, and then serum and semen samples of the boars were collected to investigate the function and molecular mechanism of NMN in sperm quality.
Show in the study text Report a problemFND-3879061203
04AnimalAnimal study (in vivo)Mechanism
In boars, NMN improved serum antioxidative indexes.
In plain wordsIn boars, NMN improved blood antioxidant measures.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Direction not reportedSerum antioxidative indexes
Study
pign = 32
Landrace boars
Comparator
0 mg/kg/d NMN
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
8, 16 or 32 mg/kg/d · oral · 9 weeksAs given in this study (pig).
Source sentences
Summary
Interestingly, NMN apparently improved the antioxidative indexes and increased the levels of testosterone (p < 0.05) in serum.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, 32 Landrace boars were randomly assigned to four groups (n = 8) and fed with different levels of NMN (0, 8, 16 or 32 mg/kg/d) for 9 weeks, and then serum and semen samples of the boars were collected to investigate the function and molecular mechanism of NMN in sperm quality.
Show in the study text Report a problemFND-3879061204
05AnimalAnimal study (in vivo)Mechanism
In boars, NMN increased serum testosterone.
In plain wordsIn boars, NMN increased blood testosterone.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseSerum testosterone
Study
pign = 32
Landrace boars
Comparator
0 mg/kg/d NMN
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
8, 16 or 32 mg/kg/d · oral · 9 weeksAs given in this study (pig).
Source sentences
Summary
Interestingly, NMN apparently improved the antioxidative indexes and increased the levels of testosterone (p < 0.05) in serum.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, 32 Landrace boars were randomly assigned to four groups (n = 8) and fed with different levels of NMN (0, 8, 16 or 32 mg/kg/d) for 9 weeks, and then serum and semen samples of the boars were collected to investigate the function and molecular mechanism of NMN in sperm quality.
Show in the study text Report a problemFND-3879061205
06AnimalAnimal study (in vivo)Mechanism
In boars, NMN increased semen SIRT3 protein levels.
In plain wordsIn boars, NMN increased SIRT3 protein in semen.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseSemen SIRT3 protein
Study
pign = 32
Landrace boars
Comparator
0 mg/kg/d NMN
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
8, 16 or 32 mg/kg/d · oral · 9 weeksAs given in this study (pig).
Source sentences
Summary
Furthermore, NMN upregulated the protein levels of sirtuin 3 (SIRT3), antioxidation and oxidative phosphorylation (OXPHOS), but downregulated the protein levels of apoptosis in semen.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, 32 Landrace boars were randomly assigned to four groups (n = 8) and fed with different levels of NMN (0, 8, 16 or 32 mg/kg/d) for 9 weeks, and then serum and semen samples of the boars were collected to investigate the function and molecular mechanism of NMN in sperm quality.
Show in the study text Report a problemFND-3879061206
07AnimalAnimal study (in vivo)Mechanism
In boars, NMN increased semen antioxidation protein levels.
In plain wordsIn boars, NMN increased antioxidant proteins in semen.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseSemen antioxidation proteins
Study
pign = 32
Landrace boars
Comparator
0 mg/kg/d NMN
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
8, 16 or 32 mg/kg/d · oral · 9 weeksAs given in this study (pig).
Source sentences
Summary
Furthermore, NMN upregulated the protein levels of sirtuin 3 (SIRT3), antioxidation and oxidative phosphorylation (OXPHOS), but downregulated the protein levels of apoptosis in semen.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, 32 Landrace boars were randomly assigned to four groups (n = 8) and fed with different levels of NMN (0, 8, 16 or 32 mg/kg/d) for 9 weeks, and then serum and semen samples of the boars were collected to investigate the function and molecular mechanism of NMN in sperm quality.
Show in the study text Report a problemFND-3879061207
08AnimalAnimal study (in vivo)Mechanism
In boars, NMN increased semen oxidative phosphorylation protein levels.
In plain wordsIn boars, NMN increased proteins involved in energy production in semen.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseSemen OXPHOS proteins
Study
pign = 32
Landrace boars
Comparator
0 mg/kg/d NMN
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
8, 16 or 32 mg/kg/d · oral · 9 weeksAs given in this study (pig).
Source sentences
Summary
Furthermore, NMN upregulated the protein levels of sirtuin 3 (SIRT3), antioxidation and oxidative phosphorylation (OXPHOS), but downregulated the protein levels of apoptosis in semen.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, 32 Landrace boars were randomly assigned to four groups (n = 8) and fed with different levels of NMN (0, 8, 16 or 32 mg/kg/d) for 9 weeks, and then serum and semen samples of the boars were collected to investigate the function and molecular mechanism of NMN in sperm quality.
Show in the study text Report a problemFND-3879061208
09AnimalAnimal study (in vivo)Mechanism
In boars, NMN decreased semen apoptosis protein levels.
In plain wordsIn boars, NMN reduced proteins associated with cell death in semen.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseSemen apoptosis proteins
Study
pign = 32
Landrace boars
Comparator
0 mg/kg/d NMN
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
8, 16 or 32 mg/kg/d · oral · 9 weeksAs given in this study (pig).
Source sentences
Summary
Furthermore, NMN upregulated the protein levels of sirtuin 3 (SIRT3), antioxidation and oxidative phosphorylation (OXPHOS), but downregulated the protein levels of apoptosis in semen.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, 32 Landrace boars were randomly assigned to four groups (n = 8) and fed with different levels of NMN (0, 8, 16 or 32 mg/kg/d) for 9 weeks, and then serum and semen samples of the boars were collected to investigate the function and molecular mechanism of NMN in sperm quality.
Show in the study text Report a problemFND-3879061209
10LabIn vitro (cells)Mechanism
In porcine sperm, NMN protected against hydrogen peroxide-induced oxidative stress through SIRT3 deacetylation.
In plain wordsIn lab tests, NMN protected pig sperm from hydrogen peroxide-induced oxidative stress through SIRT3 deacetylation.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseOxidative stress
Study
pign: not reported in source
porcine sperm exposed to hydrogen peroxide
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Mechanically, NMN protected sperm from H₂O₂-induced oxidative stress and apoptosis through SIRT3 deacetylation.
Show in the study text Report a problemFND-3879061210
11LabIn vitro (cells)Mechanism
In porcine sperm, NMN protected against hydrogen peroxide-induced apoptosis through SIRT3 deacetylation.
In plain wordsIn lab tests, NMN protected pig sperm from hydrogen peroxide-induced cell death through SIRT3 deacetylation.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseApoptosis
Study
pign: not reported in source
porcine sperm exposed to hydrogen peroxide
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Mechanically, NMN protected sperm from H₂O₂-induced oxidative stress and apoptosis through SIRT3 deacetylation.
Show in the study text Report a problemFND-3879061211

Studies that did not test the peptide alone2

In this study, NMN (nicotinamide mononucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking NMN (nicotinamide mononucleotide).

12LabNot a test of NMN (nicotinamide mononucleotide) aloneIn vitro (cells)Mechanism
In porcine sperm, SIRT3 inhibition with 3-TYP attenuated NMN-associated antioxidation.
In plain wordsIn lab tests, blocking SIRT3 reduced NMN-associated antioxidant effects in pig sperm.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseSIRT3 inhibition and antioxidation
Study
pign: not reported in source
porcine sperm
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Importantly, the SIRT3-specific inhibitor 3-TYP attenuated the antioxidation and antiapoptosis of NMN in sperm.
In this study, NMN (nicotinamide mononucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NMN (nicotinamide mononucleotide).
Show in the study text Report a problemFND-3879061212
13LabNot a test of NMN (nicotinamide mononucleotide) aloneIn vitro (cells)Mechanism
In porcine sperm, SIRT3 inhibition with 3-TYP attenuated NMN-associated antiapoptosis.
In plain wordsIn lab tests, blocking SIRT3 reduced NMN-associated protection against cell death in pig sperm.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseSIRT3 inhibition and antiapoptosis
Study
pign: not reported in source
porcine sperm
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Importantly, the SIRT3-specific inhibitor 3-TYP attenuated the antioxidation and antiapoptosis of NMN in sperm.
In this study, NMN (nicotinamide mononucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NMN (nicotinamide mononucleotide).
Show in the study text Report a problemFND-3879061213

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Keywords

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