Nicotinamide Mononucleotide improves oocyte maturation of mice with type 1 diabetes.

Guo F, Wang L, Chen Y, Zhu H, Dai X, Zhang X

Nutrition & diabetes23 Apr 2024Cited by 11 (Europe PMC)

PMID38653987PMCIDPMC11039772DOI10.1038/s41387-024-00280-8PepBankPUB-38653987

Summary

BackgroundThe number of patients with type 1 diabetes rises rapidly around the world in recent years. Maternal diabetes has a detrimental effect on reproductive outcomes due to decreased oocyte quality. However, the strategies to improve the oocyte quality and artificial reproductive technology (ART) efficiency of infertile females suffering from diabetes have not been fully studied. In this study, we aimed to examine the effects of nicotinamide mononucleotide (NMN) on oocyte maturation of mouse with type 1 diabetes mouse and explore the underlying mechanisms of NMN's effect.MethodsStreptozotocin (STZ) was used to establish the mouse models with type 1 diabetes. The successful establishment of the models was confirmed by the results of body weight test, fasting blood glucose test and haematoxylin and eosin (H&E) staining. The in vitro maturation (IVM) rate of oocytes from diabetic mice was examined. Immunofluorescence staining (IF) was performed to examine the reactive oxygen species (ROS) level, spindle/chromosome structure, mitochondrial function, actin dynamics, DNA damage and histone modification of oocytes, which are potential factors affecting the oocyte quality. The quantitative reverse transcription PCR (RT-qPCR) was used to detect the mRNA levels of Sod1, Opa1, Mfn2, Drp1, Sirt1 and Sirt3 in oocytes.ResultsThe NMN supplementation increased the oocyte maturation rate of the mice with diabetes. Furthermore, NMN supplementation improved the oocyte quality by rescuing the actin dynamics, reversing meiotic defects, improving the mitochondrial function, reducing ROS level, suppressing DNA damage and restoring changes in histone modifications of oocytes collected from the mice with diabetes.ConclusionNMN could improve the maturation rate and quality of oocytes in STZ-induced diabetic mice, which provides a significant clue for the treatment of infertility of the patients with diabetes.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueEfficacy outcome
In oocytes from diabetic mice, NMN increased maturation rate.
In plain wordsIn lab tests, NMN increased maturation of oocytes from diabetic mice.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseOocyte maturation rate
Study
mousen: not reported in source
oocytes from STZ-induced diabetic mice
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
The NMN supplementation increased the oocyte maturation rate of the mice with diabetes.
2 context sentences(design, population, supporting numbers)
Summary · context
Streptozotocin (STZ) was used to establish the mouse models with type 1 diabetes.
Summary · context
The in vitro maturation (IVM) rate of oocytes from diabetic mice was examined.
Show in the study text Report a problemFND-3865398701
02LabEx vivo tissueEfficacy outcome
In oocytes from diabetic mice, NMN rescued actin dynamics.
In plain wordsIn lab tests, NMN restored actin dynamics in oocytes from diabetic mice.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Direction not reportedActin dynamics
Study
mousen: not reported in source
oocytes from STZ-induced diabetic mice
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, NMN supplementation improved the oocyte quality by rescuing the actin dynamics, reversing meiotic defects, improving the mitochondrial function, reducing ROS level, suppressing DNA damage and restoring changes in histone modifications of oocytes collected from the mice with diabetes.
2 context sentences(design, population, supporting numbers)
Summary · context
Streptozotocin (STZ) was used to establish the mouse models with type 1 diabetes.
Summary · context
The in vitro maturation (IVM) rate of oocytes from diabetic mice was examined.
Show in the study text Report a problemFND-3865398702
03LabEx vivo tissueEfficacy outcome
In oocytes from diabetic mice, NMN reversed meiotic defects.
In plain wordsIn lab tests, NMN reduced meiotic defects in oocytes from diabetic mice.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseMeiotic defects
Study
mousen: not reported in source
oocytes from STZ-induced diabetic mice
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, NMN supplementation improved the oocyte quality by rescuing the actin dynamics, reversing meiotic defects, improving the mitochondrial function, reducing ROS level, suppressing DNA damage and restoring changes in histone modifications of oocytes collected from the mice with diabetes.
2 context sentences(design, population, supporting numbers)
Summary · context
Streptozotocin (STZ) was used to establish the mouse models with type 1 diabetes.
Summary · context
The in vitro maturation (IVM) rate of oocytes from diabetic mice was examined.
Show in the study text Report a problemFND-3865398703
04LabEx vivo tissueEfficacy outcome
In oocytes from diabetic mice, NMN improved mitochondrial function.
In plain wordsIn lab tests, NMN improved mitochondrial function in oocytes from diabetic mice.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Direction not reportedMitochondrial function
Study
mousen: not reported in source
oocytes from STZ-induced diabetic mice
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, NMN supplementation improved the oocyte quality by rescuing the actin dynamics, reversing meiotic defects, improving the mitochondrial function, reducing ROS level, suppressing DNA damage and restoring changes in histone modifications of oocytes collected from the mice with diabetes.
2 context sentences(design, population, supporting numbers)
Summary · context
Streptozotocin (STZ) was used to establish the mouse models with type 1 diabetes.
Summary · context
The in vitro maturation (IVM) rate of oocytes from diabetic mice was examined.
Show in the study text Report a problemFND-3865398704
05LabEx vivo tissueEfficacy outcome
In oocytes from diabetic mice, NMN reduced reactive oxygen species levels.
In plain wordsIn lab tests, NMN reduced reactive oxygen species in oocytes from diabetic mice.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseReactive oxygen species
Study
mousen: not reported in source
oocytes from STZ-induced diabetic mice
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, NMN supplementation improved the oocyte quality by rescuing the actin dynamics, reversing meiotic defects, improving the mitochondrial function, reducing ROS level, suppressing DNA damage and restoring changes in histone modifications of oocytes collected from the mice with diabetes.
2 context sentences(design, population, supporting numbers)
Summary · context
Streptozotocin (STZ) was used to establish the mouse models with type 1 diabetes.
Summary · context
The in vitro maturation (IVM) rate of oocytes from diabetic mice was examined.
Show in the study text Report a problemFND-3865398705
06LabEx vivo tissueEfficacy outcome
In oocytes from diabetic mice, NMN suppressed DNA damage.
In plain wordsIn lab tests, NMN reduced DNA damage in oocytes from diabetic mice.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseDNA damage
Study
mousen: not reported in source
oocytes from STZ-induced diabetic mice
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, NMN supplementation improved the oocyte quality by rescuing the actin dynamics, reversing meiotic defects, improving the mitochondrial function, reducing ROS level, suppressing DNA damage and restoring changes in histone modifications of oocytes collected from the mice with diabetes.
2 context sentences(design, population, supporting numbers)
Summary · context
Streptozotocin (STZ) was used to establish the mouse models with type 1 diabetes.
Summary · context
The in vitro maturation (IVM) rate of oocytes from diabetic mice was examined.
Show in the study text Report a problemFND-3865398706
07LabEx vivo tissueEfficacy outcome
In oocytes from diabetic mice, NMN restored altered histone modifications.
In plain wordsIn lab tests, NMN restored altered histone modifications in oocytes from diabetic mice.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Direction not reportedHistone modifications
Study
mousen: not reported in source
oocytes from STZ-induced diabetic mice
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, NMN supplementation improved the oocyte quality by rescuing the actin dynamics, reversing meiotic defects, improving the mitochondrial function, reducing ROS level, suppressing DNA damage and restoring changes in histone modifications of oocytes collected from the mice with diabetes.
2 context sentences(design, population, supporting numbers)
Summary · context
Streptozotocin (STZ) was used to establish the mouse models with type 1 diabetes.
Summary · context
The in vitro maturation (IVM) rate of oocytes from diabetic mice was examined.
Show in the study text Report a problemFND-3865398707

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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