Skip to content

Study · Animal study (in vivo) · rat · 2024

6 results

Kisspeptin-10 Improves Testicular Redox Status but Does Not Alter the Unfolded Protein Response (UPR) That Is Downregulated by Hypothyroidism in a Rat Model.

Santos LC, Dos Anjos Cordeiro JM, Cunha MCDSG, Santos BR, Oliveira LS, da Silva AL, Barbosa EM, Niella RV, de Freitas GJC, Santos DA, Serakides R, Ocarino NM, Borges SC, de Lavor MSL, Silva JF

International journal of molecular sciences26 Jan 2024Cited by 5 (Europe PMC)

PMID38338793 (opens in a new tab)PMCIDPMC10855899 (opens in a new tab)DOI10.3390/ijms25031514 (opens in a new tab)PepBankPUB-38338793
Download PDFPubMedAsk about this study
Jump to 6 results

Summary

Hypothyroidism compromises the testicular redox status and is associated with reduced sperm quality and infertility in men. In this regard, studies have demonstrated the antioxidant potential of kisspeptin in reproductive and metabolic diseases. In this study, we evaluate the effects of kisspeptin-10 (Kp10) on the testicular redox, as well as mediators of the unfolded protein response (UPR) in adult rats with hypothyroidism. Adult male Wistar rats were randomly separated into the Control (n = 15), Hypo (n = 13) and Hypo + Kp10 (n = 14) groups, and hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months. In the last month, half of the hypothyroid animals received Kp10. Testis samples were collected for enzymatic, immunohistochemical and/or gene evaluation of mediators of oxidative stress (TBARs, lipid hydroperoxides (LOOH), ROS, peroxynitrite, SOD, CAT and GPX), endoplasmic reticulum stress (GRP78, ATF6, PERK, CHOP, HO-1 and sXBP1) and antiapoptocytes (BCL-2). Hypothyroidism increased apoptosis index, TBARS and LOOH concentrations, and reduced testicular gene expression of Sod1, Sod2 and Gpx1, as well as the expression of Grp78, Atf6, Ho1 and Chop. Treatment with Kp10, in turn, reduced testicular apoptosis and the production of peroxynitrite, while increased SOD1 and GPX ½ expression, and enzymatic activity of CAT, but did not affect the lower expression of UPR mediators caused by hypothyroidism. This study demonstrated that hypothyroidism causes oxidative stress and dysregulated the UPR pathway in rat testes and that, although Kp10 does not influence the low expression of UPR mediators, it improves the testicular redox status, configuring it as an important antioxidant factor in situations of thyroid dysfunction.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In hypothyroid rats, Kp10 reduced testicular apoptosis.

    In plain wordsIn rats with an underactive thyroid, kisspeptin‑10 lowered cell death in the testis.

    Peptide
    Kisspeptin-10
    Outcome
    DecreaseTesticular apoptosis
    Study

    ratn: not reported in source

    PTU-induced hypothyroid male Wistar rats
    Comparator
    Hypothyroid rats without Kp10
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated · 1 monthAs given in this study (rat).

    Source sentences

    Summary
    Treatment with Kp10, in turn, reduced testicular apoptosis and the production of peroxynitrite, while increased SOD1 and GPX ½ expression, and enzymatic activity of CAT, but did not affect the lower expression of UPR mediators caused by hypothyroidism.
    1 context sentence
    Summary · context
    Adult male Wistar rats were randomly separated into the Control (n = 15), Hypo (n = 13) and Hypo + Kp10 (n = 14) groups, and hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In hypothyroid rats, Kp10 reduced testicular peroxynitrite production.

    In plain wordsIn rats with an underactive thyroid, kisspeptin‑10 lowered a harmful oxidant in the testis.

    Peptide
    Kisspeptin-10
    Outcome
    DecreaseTesticular peroxynitrite production
    Study

    ratn: not reported in source

    PTU-induced hypothyroid male Wistar rats
    Comparator
    Hypothyroid rats without Kp10
    Outcome group
    Oxidative stress · Reproductive and sexual health
    Studied dose
    Dose not stated · 1 monthAs given in this study (rat).

    Source sentences

    Summary
    Treatment with Kp10, in turn, reduced testicular apoptosis and the production of peroxynitrite, while increased SOD1 and GPX ½ expression, and enzymatic activity of CAT, but did not affect the lower expression of UPR mediators caused by hypothyroidism.
    1 context sentence
    Summary · context
    Adult male Wistar rats were randomly separated into the Control (n = 15), Hypo (n = 13) and Hypo + Kp10 (n = 14) groups, and hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.
  3. 03AnimalAnimal study (in vivo)Efficacy outcome

    In hypothyroid rats, Kp10 increased testicular SOD1 expression.

    In plain wordsIn rats with an underactive thyroid, kisspeptin‑10 raised an antioxidant enzyme in the testis.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseTesticular SOD1 expression
    Study

    ratn: not reported in source

    PTU-induced hypothyroid male Wistar rats
    Comparator
    Hypothyroid rats without Kp10
    Outcome group
    Oxidative stress · Reproductive and sexual health
    Studied dose
    Dose not stated · 1 monthAs given in this study (rat).

    Source sentences

    Summary
    Treatment with Kp10, in turn, reduced testicular apoptosis and the production of peroxynitrite, while increased SOD1 and GPX ½ expression, and enzymatic activity of CAT, but did not affect the lower expression of UPR mediators caused by hypothyroidism.
    1 context sentence
    Summary · context
    Adult male Wistar rats were randomly separated into the Control (n = 15), Hypo (n = 13) and Hypo + Kp10 (n = 14) groups, and hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.
  4. 04AnimalAnimal study (in vivo)Efficacy outcome

    In hypothyroid rats, Kp10 increased testicular GPX 1/2 expression.

    In plain wordsIn rats with an underactive thyroid, kisspeptin‑10 raised an antioxidant enzyme in the testis.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseTesticular GPX 1/2 expression
    Study

    ratn: not reported in source

    PTU-induced hypothyroid male Wistar rats
    Comparator
    Hypothyroid rats without Kp10
    Outcome group
    Oxidative stress · Reproductive and sexual health
    Studied dose
    Dose not stated · 1 monthAs given in this study (rat).

    Source sentences

    Summary
    Treatment with Kp10, in turn, reduced testicular apoptosis and the production of peroxynitrite, while increased SOD1 and GPX ½ expression, and enzymatic activity of CAT, but did not affect the lower expression of UPR mediators caused by hypothyroidism.
    1 context sentence
    Summary · context
    Adult male Wistar rats were randomly separated into the Control (n = 15), Hypo (n = 13) and Hypo + Kp10 (n = 14) groups, and hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.
  5. 05AnimalAnimal study (in vivo)Efficacy outcome

    In hypothyroid rats, Kp10 increased testicular catalase activity.

    In plain wordsIn rats with an underactive thyroid, kisspeptin‑10 raised the activity of an antioxidant enzyme in the testis.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseTesticular catalase (CAT) enzymatic activity
    Study

    ratn: not reported in source

    PTU-induced hypothyroid male Wistar rats
    Comparator
    Hypothyroid rats without Kp10
    Outcome group
    Oxidative stress · Reproductive and sexual health
    Studied dose
    Dose not stated · 1 monthAs given in this study (rat).

    Source sentences

    Summary
    Treatment with Kp10, in turn, reduced testicular apoptosis and the production of peroxynitrite, while increased SOD1 and GPX ½ expression, and enzymatic activity of CAT, but did not affect the lower expression of UPR mediators caused by hypothyroidism.
    1 context sentence
    Summary · context
    Adult male Wistar rats were randomly separated into the Control (n = 15), Hypo (n = 13) and Hypo + Kp10 (n = 14) groups, and hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.
  6. 06AnimalAnimal study (in vivo)Efficacy outcome

    In hypothyroid rats, Kp10 did not affect the low expression of unfolded protein response mediators caused by hypothyroidism.

    In plain wordsIn rats with an underactive thyroid, kisspeptin‑10 did not change the low level of stress-response proteins in the testis.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeUnfolded protein response mediators
    Study

    ratn: not reported in source

    PTU-induced hypothyroid male Wistar rats
    Comparator
    Hypothyroid rats without Kp10
    Outcome group
    Oxidative stress · Reproductive and sexual health
    Studied dose
    Dose not stated · 1 monthAs given in this study (rat).

    Source sentences

    Summary
    Treatment with Kp10, in turn, reduced testicular apoptosis and the production of peroxynitrite, while increased SOD1 and GPX ½ expression, and enzymatic activity of CAT, but did not affect the lower expression of UPR mediators caused by hypothyroidism.
    1 context sentence
    Summary · context
    Adult male Wistar rats were randomly separated into the Control (n = 15), Hypo (n = 13) and Hypo + Kp10 (n = 14) groups, and hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.