Regulatory Effects of the Kiss1 Gene in the Testis on Puberty and Reproduction in Hezuo and Landrance Boars.

Shi H, Yan Z, Du H, Tang Y, Song K, Yang Q, Huang X, Wang P, Gao X, Yang J, Gun S

International journal of molecular sciences24 Nov 2023Cited by 3 (Europe PMC)

PMID38069021PMCIDPMC10705963DOI10.3390/ijms242316700PepBankPUB-38069021

Summary

Kisspeptin, a neuropeptide encoded by the Kiss1 gene, combines with its receptor Kiss1R to regulate the onset of puberty and male fertility by the hypothalamic-pituitary-gonadal axis. However, little is known regarding the expression signatures and molecular functions of Kiss1 in the testis. H&E staining revealed that well-arranged spermatogonia, spermatocytes, round and elongated spermatids, and spermatozoa, were observed in 4-, 6-, and 8-month-old testes compared to 1- and 3-month-old testes of Hezuo pigs; however, these were not observed in Landrance until 6 months. The diameter, perimeter, and cross-sectional area of seminiferous tubules and the perimeter and area of the tubular lumen increased gradually with age in both pigs. Still, Hezuo pigs grew faster than Landrance. The cloning results suggested that the Hezuo pigs' Kiss1 CDS region is 417 bp in length, encodes 138 amino acids, and is highly conserved in the kisspeptin-10 region. qRT-PCR and Western blot indicated that the expression trends of Kiss1 mRNA and protein were essentially identical, with higher expression levels at post-pubertal stages. Immunohistochemistry demonstrated that the Kiss1 protein was mainly located in Leydig cells and post-pubertal spermatogenic cells, ranging from round spermatids to spermatozoa. These studies suggest that Kiss1 is an essential regulator in the onset of puberty and spermatogenesis of boars.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study3

No result from this study comes from a test of the peptide.

Natural levels measured2

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01AnimalEndogenous levelAnimal study (in vivo)Other
In Hezuo boars, testicular Kiss1 mRNA and protein expression was higher at post-pubertal stages, so this describes the body's own Kiss1 product.
In plain wordsIn pigs, the body's own kisspeptin gene product was higher in the testis after puberty.
Peptide
Kisspeptin-10
Outcome
IncreaseKiss1 mRNA and protein expression in the testis by age
Study
pign: not reported in source
Hezuo and Landrance boars, testis
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
The cloning results suggested that the Hezuo pigs' Kiss1 CDS region is 417 bp in length, encodes 138 amino acids, and is highly conserved in the kisspeptin-10 region. qRT-PCR and Western blot indicated that the expression trends of Kiss1 mRNA and protein were essentially identical, with higher expression levels at post-pubertal stages.
1 context sentence(design, population, supporting numbers)
Title · context
Regulatory Effects of the Kiss1 Gene in the Testis on Puberty and Reproduction in Hezuo and Landrance Boars.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-3806902101
02AnimalEndogenous levelAnimal study (in vivo)Other
In boars, the body's own Kiss1 protein was mainly located in Leydig cells and post-pubertal spermatogenic cells.
In plain wordsIn pigs, the body's own kisspeptin protein was found mainly in testis Leydig cells and maturing sperm cells.
Peptide
Kisspeptin-10
Outcome
Not applicableLocalization of Kiss1 protein in the testis
Study
pign: not reported in source
Hezuo and Landrance boars, testis
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Immunohistochemistry demonstrated that the Kiss1 protein was mainly located in Leydig cells and post-pubertal spermatogenic cells, ranging from round spermatids to spermatozoa.
1 context sentence(design, population, supporting numbers)
Title · context
Regulatory Effects of the Kiss1 Gene in the Testis on Puberty and Reproduction in Hezuo and Landrance Boars.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-3806902102

Studies that did not test the peptide alone1

In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Kisspeptin-10.

03AnimalNot a test of Kisspeptin-10 aloneAnimal study (in vivo)Other
In Hezuo pigs, the cloned Kiss1 coding region encodes 138 amino acids and is highly conserved in the kisspeptin‑10 region.
In plain wordsIn pigs, the kisspeptin gene sequence is highly similar to other species in the kisspeptin‑10 region.
Peptide
Kisspeptin-10
Outcome
Not applicableKiss1 coding sequence conservation
Study
pign: not reported in source
Hezuo and Landrance boars, testis
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Measurements
Kiss1 CDS length
417 bp
Hezuo pigs
Source sentences
Summary
The cloning results suggested that the Hezuo pigs' Kiss1 CDS region is 417 bp in length, encodes 138 amino acids, and is highly conserved in the kisspeptin-10 region. qRT-PCR and Western blot indicated that the expression trends of Kiss1 mRNA and protein were essentially identical, with higher expression levels at post-pubertal stages.
1 context sentence(design, population, supporting numbers)
Title · context
Regulatory Effects of the Kiss1 Gene in the Testis on Puberty and Reproduction in Hezuo and Landrance Boars.
In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-10.
Show in the study text Report a problemFND-3806902103

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

MeSH terms

Keywords

PepBank is a research reference. Talk to a doctor before you use any peptide.