Effects of Humanin G (HNG) on angiogenesis and neurodegeneration markers in Age-related Macular Degeneration (AMD).

Nashine S, Kenney MC

Mitochondrion27 Nov 2023Cited by 6 (Europe PMC)

PMID38029849DOI10.1016/j.mito.2023.11.001PepBankPUB-38029849

Publisher page PubMedAsk about this study

Summary

Advanced stages of Age-related Macular Degeneration (AMD) are characterized by retinal neurodegeneration and aberrant angiogenesis, and mitochondrial dysfunction contributes to the pathogenesis of AMD. In this study, we tested the hypothesis that Humanin G (HNG), a cytoprotective mitochondrial-derived peptide, positively regulates cell proliferation, cell death, and the protein levels of angiogenesis and neurodegeneration markers, in normal (control) and AMD RPE transmitochondrial cybrid cell lines. These normal and AMD RPE transmitochondrial cybrid cell lines had identical nuclei derived from mitochondria-deficient ARPE-19 cell line, but differed in mitochondrial DNA (mtDNA) content that was derived from clinically characterized AMD patients and normal (control) subjects. Cell lysates were extracted from untreated and HNG-treated AMD and normal (control) cybrid cell lines, and the Luminex XMAP multiplex assay was used to examine the protein levels of angiogenesis and neurodegeneration markers. Humanin G reduced Caspase-3/7-mediated apoptosis, improved cell proliferation, and normalized the protein levels of angiogenesis and neurodegeneration markers in AMD RPE cybrid cell lines, thereby suggesting Humanin G's positive regulatory role in AMD.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In AMD RPE cybrid cell lines, Humanin G reduced Caspase-3/7-mediated apoptosis.
In plain wordsIn lab cells that model macular degeneration, Humanin G reduced a form of cell death.
Peptide
Humanin
Outcome
DecreaseCaspase-3/7-mediated apoptosis
Study
humann: not reported in source
AMD and normal RPE transmitochondrial cybrid cell lines
Outcome group
Cell viability and death · Eye
Studied dose
Dose not stated
Source sentences
Summary
Humanin G reduced Caspase-3/7-mediated apoptosis
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we tested the hypothesis that Humanin G (HNG), a cytoprotective mitochondrial-derived peptide, positively regulates cell proliferation, cell death, and the protein levels of angiogenesis and neurodegeneration markers, in normal (control) and AMD RPE transmitochondrial cybrid cell lines.
Show in the study text Report a problemFND-3802984901
02LabIn vitro (cells)Efficacy outcome
In AMD RPE cybrid cell lines, Humanin G improved cell proliferation.
In plain wordsIn lab cells that model macular degeneration, Humanin G improved cell growth.
Peptide
Humanin
Outcome
IncreaseCell proliferation
Study
humann: not reported in source
AMD and normal RPE transmitochondrial cybrid cell lines
Outcome group
Cell viability and death · Eye
Studied dose
Dose not stated
Source sentences
Summary
Humanin G reduced Caspase-3/7-mediated apoptosis, improved cell proliferation
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we tested the hypothesis that Humanin G (HNG), a cytoprotective mitochondrial-derived peptide, positively regulates cell proliferation, cell death, and the protein levels of angiogenesis and neurodegeneration markers, in normal (control) and AMD RPE transmitochondrial cybrid cell lines.
Show in the study text Report a problemFND-3802984902
03LabIn vitro (cells)Efficacy outcome
In AMD RPE cybrid cell lines, Humanin G normalized the protein levels of angiogenesis markers.
In plain wordsIn lab cells that model macular degeneration, Humanin G brought blood-vessel growth markers back toward normal levels.
Peptide
Humanin
Outcome
MixedAngiogenesis marker protein levels
Study
humann: not reported in source
AMD and normal RPE transmitochondrial cybrid cell lines
Outcome group
Angiogenesis · Eye
Studied dose
Dose not stated
Source sentences
Summary
Humanin G reduced Caspase-3/7-mediated apoptosis, improved cell proliferation, and normalized the protein levels of angiogenesis and neurodegeneration markers in AMD RPE cybrid cell lines
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we tested the hypothesis that Humanin G (HNG), a cytoprotective mitochondrial-derived peptide, positively regulates cell proliferation, cell death, and the protein levels of angiogenesis and neurodegeneration markers, in normal (control) and AMD RPE transmitochondrial cybrid cell lines.
Show in the study text Report a problemFND-3802984903
04LabIn vitro (cells)Efficacy outcome
In AMD RPE cybrid cell lines, Humanin G normalized the protein levels of neurodegeneration markers.
In plain wordsIn lab cells that model macular degeneration, Humanin G brought nerve-damage markers back toward normal levels.
Peptide
Humanin
Outcome
MixedNeurodegeneration marker protein levels
Study
humann: not reported in source
AMD and normal RPE transmitochondrial cybrid cell lines
Outcome group
Neuroprotection · Eye
Studied dose
Dose not stated
Source sentences
Summary
Humanin G reduced Caspase-3/7-mediated apoptosis, improved cell proliferation, and normalized the protein levels of angiogenesis and neurodegeneration markers in AMD RPE cybrid cell lines
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we tested the hypothesis that Humanin G (HNG), a cytoprotective mitochondrial-derived peptide, positively regulates cell proliferation, cell death, and the protein levels of angiogenesis and neurodegeneration markers, in normal (control) and AMD RPE transmitochondrial cybrid cell lines.
Show in the study text Report a problemFND-3802984904

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

MeSH terms

Keywords

PepBank is a research reference. Talk to a doctor before you use any peptide.