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Study · Animal study (in vivo) · mouse · 2023

9 results

Kisspeptin-10 increases collagen content in the myocardium by focal adhesion kinase activity.

Radwańska P, Gałdyszyńska M, Piera L, Drobnik J

Scientific reports15 Nov 2023Cited by 5 (Europe PMC)

PMID37968564 (opens in a new tab)PMCIDPMC10651918 (opens in a new tab)DOI10.1038/s41598-023-47224-3 (opens in a new tab)PepBankPUB-37968564
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Summary

The aim of the study was to evaluate the role of kisspeptin-10 (KiSS-10) in the regulation of collagen content in cardiac fibroblasts. An attempt was also made to describe the mechanism of the effect of KiSS-10 on collagen metabolism. The studies indicate that kisspeptin-10 significantly increases the content of intracellular collagen in the myocardium. KiSS-10 also elevates the level of phosphorylated focal adhesion kinase (FAK) in human cardiac fibroblasts. The inhibition of FAK negates the stimulatory effect of KiSS-10 on collagen deposition in vitro. These changes correlate with an increase in the level of propeptides of procollagen type I (PICP) and III (PIIICP) in fibroblast culture medium and mouse PIIICP in serum. Moreover, this hormone inhibits the release of metalloproteinases (MMP-1,-2,-9) and elevates the secretion of their tissue inhibitors (TIMP-1,-2,-4). KiSS-10 also enhances the expression of α1 chains of procollagen type I and III in vitro. Thus, KiSS-10 is involved in the regulation of collagen metabolism and cardiac fibrosis. Augmentation of collagen deposition by KiSS-10 is dependent on the protein synthesis elevation, inhibition of MMPs activity (increase of TIMPs release) or decrease of MMPs concentration. The profibrotic activity of KiSS-10 is mediated by FAK and is not dependent on TGF-β1.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study9

9 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In mice, the KiSS-10 effects correlated with an increase in serum PIIICP.

    In plain wordsIn mice, kisspeptin‑10 effects were linked to a higher blood level of a collagen production marker.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseMouse serum PIIICP
    Study

    mousen: not reported in source

    mice
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    These changes correlate with an increase in the level of propeptides of procollagen type I (PICP) and III (PIIICP) in fibroblast culture medium and mouse PIIICP in serum.
    1 context sentence
    Summary · context
    The aim of the study was to evaluate the role of kisspeptin-10 (KiSS-10) in the regulation of collagen content in cardiac fibroblasts.
  2. 02LabIn vitro (cells)Efficacy outcome

    In cardiac fibroblast studies, kisspeptin‑10 significantly increased intracellular collagen content.

    In plain wordsIn lab tests on heart fibroblast cells, kisspeptin‑10 increased the collagen inside the cells.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseIntracellular collagen content in the myocardium
    Study

    n: not reported in source

    cardiac fibroblasts
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    Dose not stated · in vitroAs given in this study.

    Source sentences

    Summary
    The studies indicate that kisspeptin-10 significantly increases the content of intracellular collagen in the myocardium.
    1 context sentence
    Summary · context
    The aim of the study was to evaluate the role of kisspeptin-10 (KiSS-10) in the regulation of collagen content in cardiac fibroblasts.
  3. 03LabIn vitro (cells)Mechanism

    In human cardiac fibroblasts, KiSS-10 elevated the level of phosphorylated focal adhesion kinase (FAK).

    In plain wordsIn human heart cells grown in a dish, kisspeptin‑10 raised the level of the active form of the enzyme FAK.

    Peptide
    Kisspeptin-10
    Outcome
    IncreasePhosphorylated FAK in human cardiac fibroblasts
    Study

    humann: not reported in source

    cardiac fibroblasts
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    Dose not stated · in vitroAs given in this study (human).

    Source sentences

    Summary
    KiSS-10 also elevates the level of phosphorylated focal adhesion kinase (FAK) in human cardiac fibroblasts.
    1 context sentence
    Summary · context
    The aim of the study was to evaluate the role of kisspeptin-10 (KiSS-10) in the regulation of collagen content in cardiac fibroblasts.
  4. 04LabIn vitro (cells)Mechanism

    In vitro, inhibition of FAK negated the stimulatory effect of KiSS-10 on collagen deposition.

    In plain wordsIn lab tests, blocking FAK stopped kisspeptin‑10 from increasing collagen buildup.

    Peptide
    Kisspeptin-10
    Outcome
    Not applicableFAK dependence of KiSS-10 effect on collagen deposition
    Study

    n: not reported in source

    cardiac fibroblasts
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    Dose not stated · in vitroAs given in this study.

    Source sentences

    Summary
    The inhibition of FAK negates the stimulatory effect of KiSS-10 on collagen deposition in vitro.
    1 context sentence
    Summary · context
    The aim of the study was to evaluate the role of kisspeptin-10 (KiSS-10) in the regulation of collagen content in cardiac fibroblasts.
  5. 05LabIn vitro (cells)Efficacy outcome

    In fibroblast culture, KiSS-10 increased PICP and PIIICP propeptides of procollagen type I and III in the medium.

    In plain wordsIn cells grown in a dish, kisspeptin‑10 raised markers of new collagen production in the culture fluid.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseProcollagen propeptides PICP and PIIICP in culture medium
    Study

    n: not reported in source

    cardiac fibroblasts
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    Dose not stated · in vitroAs given in this study.

    Source sentences

    Summary
    These changes correlate with an increase in the level of propeptides of procollagen type I (PICP) and III (PIIICP) in fibroblast culture medium
    2 context sentences
    Summary · context
    KiSS-10 also elevates the level of phosphorylated focal adhesion kinase (FAK) in human cardiac fibroblasts.
    Summary · context
    The aim of the study was to evaluate the role of kisspeptin-10 (KiSS-10) in the regulation of collagen content in cardiac fibroblasts.
  6. 06LabIn vitro (cells)Efficacy outcome

    In vitro, KiSS-10 inhibited the release of metalloproteinases MMP-1, -2 and -9.

    In plain wordsIn lab tests, kisspeptin‑10 reduced the release of enzymes that break down collagen.

    Peptide
    Kisspeptin-10
    Outcome
    DecreaseMetalloproteinase (MMP-1, -2, -9) release
    Study

    n: not reported in source

    cardiac fibroblasts
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    Dose not stated · in vitroAs given in this study.

    Source sentences

    Summary
    Moreover, this hormone inhibits the release of metalloproteinases (MMP-1,-2,-9) and elevates the secretion of their tissue inhibitors (TIMP-1,-2,-4).
    1 context sentence
    Summary · context
    The aim of the study was to evaluate the role of kisspeptin-10 (KiSS-10) in the regulation of collagen content in cardiac fibroblasts.
  7. 07LabIn vitro (cells)Efficacy outcome

    In vitro, KiSS-10 elevated the secretion of the tissue inhibitors of metalloproteinases TIMP-1, -2 and -4.

    In plain wordsIn lab tests, kisspeptin‑10 raised the release of proteins that block collagen-degrading enzymes.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseTIMP-1, -2, -4 secretion
    Study

    n: not reported in source

    cardiac fibroblasts
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    Dose not stated · in vitroAs given in this study.

    Source sentences

    Summary
    Moreover, this hormone inhibits the release of metalloproteinases (MMP-1,-2,-9) and elevates the secretion of their tissue inhibitors (TIMP-1,-2,-4).
    1 context sentence
    Summary · context
    The aim of the study was to evaluate the role of kisspeptin-10 (KiSS-10) in the regulation of collagen content in cardiac fibroblasts.
  8. 08LabIn vitro (cells)Efficacy outcome

    In vitro, KiSS-10 enhanced the expression of α1 chains of procollagen type I and III.

    In plain wordsIn lab tests, kisspeptin‑10 increased the activity of genes that make collagen building blocks.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseProcollagen type I and III α1 chain expression
    Study

    n: not reported in source

    cardiac fibroblasts
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    Dose not stated · in vitroAs given in this study.

    Source sentences

    Summary
    KiSS-10 also enhances the expression of α1 chains of procollagen type I and III in vitro.
    1 context sentence
    Summary · context
    The aim of the study was to evaluate the role of kisspeptin-10 (KiSS-10) in the regulation of collagen content in cardiac fibroblasts.
  9. 09LabIn vitro (cells)Mechanism

    In vitro, the profibrotic activity of KiSS-10 was mediated by FAK and was not dependent on TGF-β1.

    In plain wordsIn lab tests, kisspeptin‑10's collagen-building effect worked through FAK and did not depend on TGF-β1.

    Peptide
    Kisspeptin-10
    Outcome
    Not applicableTGF-β1 dependence of KiSS-10 profibrotic activity
    Study

    n: not reported in source

    cardiac fibroblasts
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    Dose not stated · in vitroAs given in this study.

    Source sentences

    Summary
    The profibrotic activity of KiSS-10 is mediated by FAK and is not dependent on TGF-β1.
    1 context sentence
    Summary · context
    The aim of the study was to evaluate the role of kisspeptin-10 (KiSS-10) in the regulation of collagen content in cardiac fibroblasts.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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