β-Nicotinamide Mononucleotide Alleviates Hydrogen Peroxide-Induced Cell Cycle Arrest and Death in Ovarian Granulosa Cells.

Wang Y, Li Q, Ma Z, Xu H, Peng F, Chen B, Ma B, Qin L, Lan J, Li Y, Lan D, Li J, Wang S, Fu W

International journal of molecular sciences27 Oct 2023Cited by 6 (Europe PMC)

PMID37958650PMCIDPMC10649918DOI10.3390/ijms242115666PepBankPUB-37958650

Summary

Maintaining normal functions of ovarian granulosa cells (GCs) is essential for oocyte development and maturation. The dysfunction of GCs impairs nutrition supply and estrogen secretion by follicles, thus negatively affecting the breeding capacity of farm animals. Impaired GCs is generally associated with declines in Nicotinamide adenine dinucleotide (NAD⁺) levels, which triggers un-controlled oxidative stress, and the oxidative stress, thus, attack the subcellular structures and cause cell damage. β-nicotinamide mononucleotide (NMN), a NAD⁺ precursor, has demonstrated well-known antioxidant properties in several studies. In this study, using two types of ovarian GCs (mouse GCs (mGCs) and human granulosa cell line (KGN)) as cell models, we aimed to investigate the potential effects of NMN on gene expression patterns and antioxidant capacity of both mGCs and KGN that were exposed to hydrogen peroxide (H₂O₂). As shown in results of the study, mGCs that were exposed to H₂O₂ significantly altered the gene expression patterns, with 428 differentially expressed genes (DEGs) when compared with those of the control group. Furthermore, adding NMN to H₂O₂-cultured mGCs displayed 621 DEGs. The functional enrichment analysis revealed that DEGs were mainly enriched in key pathways like cell cycle, senescence, and cell death. Using RT-qPCR, CCK8, and β-galactosidase staining, we found that H₂O₂ exposure on mGCs obviously reduced cell activity/mRNA expressions of antioxidant genes, inhibited cell proliferation, and induced cellular senescence. Notably, NMN supplementation partially prevented these H₂O₂-induced abnormalities. Moreover, these similar beneficial effects of NMN on antioxidant capacity were confirmed in the KGN cell models that were exposed to H₂O₂. Taken together, the present results demonstrate that NMN supplementation protects against H₂O₂-induced impairments in gene expression pattern, cell cycle arrest, and cell death in ovarian GCs through boosting NAD⁺ levels and provide potential strategies to ameliorate uncontrolled oxidative stress in ovarian GCs.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study7

6 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In peroxide-exposed mouse granulosa cells, adding NMN yielded 621 differentially expressed genes.
In plain wordsIn mouse cells exposed to peroxide, adding NMN yielded 621 differentially expressed genes.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Direction not reportedDifferential gene expression
Study
mousen: not reported in source
H2O2-exposed ovarian granulosa cells
Outcome group
Cell viability and death · Reproductive and sexual health
Studied dose
Dose not stated
Measurements
Differentially expressed genes
621 genes
NMN added to H2O2-cultured mouse granulosa cells
Source sentences
Summary
Furthermore, adding NMN to H₂O₂-cultured mGCs displayed 621 DEGs.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, using two types of ovarian GCs (mouse GCs (mGCs) and human granulosa cell line (KGN)) as cell models, we aimed to investigate the potential effects of NMN on gene expression patterns and antioxidant capacity of both mGCs and KGN that were exposed to hydrogen peroxide (H₂O₂).
Show in the study text Report a problemFND-3795865001
02LabIn vitro (cells)Mechanism
In peroxide-exposed mouse granulosa cells, NMN partially prevented reduced cell activity.
In plain wordsIn mouse cells exposed to peroxide, NMN partly prevented reduced cell activity.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseCell activity
Study
mousen: not reported in source
H2O2-exposed ovarian granulosa cells
Outcome group
Cell viability and death · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Notably, NMN supplementation partially prevented these H₂O₂-induced abnormalities.
1 context sentence(design, population, supporting numbers)
Summary · context
Using RT-qPCR, CCK8, and β-galactosidase staining, we found that H₂O₂ exposure on mGCs obviously reduced cell activity/mRNA expressions of antioxidant genes, inhibited cell proliferation, and induced cellular senescence.
Show in the study text Report a problemFND-3795865003
03LabIn vitro (cells)Mechanism
In peroxide-exposed mouse granulosa cells, NMN partially prevented reduced antioxidant gene mRNA expression.
In plain wordsIn mouse cells exposed to peroxide, NMN partly prevented reduced antioxidant gene expression.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseAntioxidant gene mRNA
Study
mousen: not reported in source
H2O2-exposed ovarian granulosa cells
Outcome group
Cell viability and death · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Notably, NMN supplementation partially prevented these H₂O₂-induced abnormalities.
1 context sentence(design, population, supporting numbers)
Summary · context
Using RT-qPCR, CCK8, and β-galactosidase staining, we found that H₂O₂ exposure on mGCs obviously reduced cell activity/mRNA expressions of antioxidant genes, inhibited cell proliferation, and induced cellular senescence.
Show in the study text Report a problemFND-3795865004
04LabIn vitro (cells)Mechanism
In peroxide-exposed mouse granulosa cells, NMN partially prevented inhibition of cell proliferation.
In plain wordsIn mouse cells exposed to peroxide, NMN partly prevented reduced cell proliferation.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseCell proliferation
Study
mousen: not reported in source
H2O2-exposed ovarian granulosa cells
Outcome group
Cell viability and death · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Notably, NMN supplementation partially prevented these H₂O₂-induced abnormalities.
1 context sentence(design, population, supporting numbers)
Summary · context
Using RT-qPCR, CCK8, and β-galactosidase staining, we found that H₂O₂ exposure on mGCs obviously reduced cell activity/mRNA expressions of antioxidant genes, inhibited cell proliferation, and induced cellular senescence.
Show in the study text Report a problemFND-3795865005
05LabIn vitro (cells)Mechanism
In peroxide-exposed mouse granulosa cells, NMN partially prevented cellular senescence.
In plain wordsIn mouse cells exposed to peroxide, NMN partly prevented cell aging.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseCellular senescence
Study
mousen: not reported in source
H2O2-exposed ovarian granulosa cells
Outcome group
Cell viability and death · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Notably, NMN supplementation partially prevented these H₂O₂-induced abnormalities.
1 context sentence(design, population, supporting numbers)
Summary · context
Using RT-qPCR, CCK8, and β-galactosidase staining, we found that H₂O₂ exposure on mGCs obviously reduced cell activity/mRNA expressions of antioxidant genes, inhibited cell proliferation, and induced cellular senescence.
Show in the study text Report a problemFND-3795865006
06LabIn vitro (cells)Mechanism
In peroxide-exposed KGN cells, NMN had beneficial effects on antioxidant capacity.
In plain wordsIn human KGN cells exposed to peroxide, NMN improved antioxidant capacity.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseAntioxidant capacity
Study
humann: not reported in source
H2O2-exposed KGN granulosa cell line
Outcome group
Cell viability and death · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Moreover, these similar beneficial effects of NMN on antioxidant capacity were confirmed in the KGN cell models that were exposed to H₂O₂.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, using two types of ovarian GCs (mouse GCs (mGCs) and human granulosa cell line (KGN)) as cell models, we aimed to investigate the potential effects of NMN on gene expression patterns and antioxidant capacity of both mGCs and KGN that were exposed to hydrogen peroxide (H₂O₂).
Show in the study text Report a problemFND-3795865007

Studies that did not test the peptide alone1

In this study, NMN (nicotinamide mononucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking NMN (nicotinamide mononucleotide).

07Computer modelNot a test of NMN (nicotinamide mononucleotide) aloneIn silicoMechanism
Functional enrichment analysis identified cell cycle, senescence, and cell death pathways among differentially expressed genes in the granulosa cell experiments.
In plain wordsComputer analysis identified cell cycle, aging, and cell death pathways among genes from the granulosa cell experiments.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Not applicableEnriched pathways
Study
mousen: not reported in source
Functional enrichment analysis of differentially expressed genes
Outcome group
Cell viability and death · Reproductive and sexual health
Source sentences
Summary
The functional enrichment analysis revealed that DEGs were mainly enriched in key pathways like cell cycle, senescence, and cell death.
1 context sentence(design, population, supporting numbers)
Summary · context
Furthermore, adding NMN to H₂O₂-cultured mGCs displayed 621 DEGs.
In this study, NMN (nicotinamide mononucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NMN (nicotinamide mononucleotide).
Show in the study text Report a problemFND-3795865002

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

MeSH terms

Keywords

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