Kisspeptin (Kp-10) inhibits in vitro osteogenic differentiation of multipotent mesenchymal stromal cells extracted from the bone marrow of adult rats.

Guimarães LB, Machado DPD, Carvalho Versiani Caldeira BF, Vieira LTM, Santos GA, Araújo FR, Machado LT, Gomes DA, Ocarino NM, Serakides R, Reis AMS

Acta histochemica8 Nov 2023Cited by 2 (Europe PMC)

PMID37948785DOI10.1016/j.acthis.2023.152112PepBankPUB-37948785

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Summary

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Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study9

9 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In rat bone marrow MSCs, Kp-10 reduced in vitro osteogenic differentiation.
In plain wordsIn rat cells grown in a dish, kisspeptin‑10 reduced the change of stem cells into bone-forming cells.
Peptide
Kisspeptin-10
Outcome
DecreaseOsteogenic differentiation of MSCs
Study
ratn: not reported in source
bone marrow mesenchymal stromal cells from adult Wistar rats
Outcome group
Bone and joint · Musculoskeletal
Studied dose
0.01, 0.05 and 0.1 µg/mL · in vitroAs given in this study (rat).
Source sentences
Summary
It was observed that MSCs expressed GPR54 receptor to which Kp-10 binds during osteogenic differentiation, promoting a negative effect on osteogenic differentiation.
1 context sentence(design, population, supporting numbers)
Summary · context
Four experimental groups were established in vitro: a control and Kp-10 at concentrations of 0.01, 0.05 and, 0.1 µg/mL.
Report a problemFND-3794878501
02LabIn vitro (cells)Mechanism
In rat bone marrow MSCs, the GPR54 receptor to which Kp-10 binds was expressed during osteogenic differentiation.
In plain wordsIn rat cells grown in a dish, the receptor that kisspeptin‑10 binds to was present during bone-cell differentiation.
Peptide
Kisspeptin-10
Outcome
Not applicableGPR54 receptor expression in MSCs
Study
ratn: not reported in source
bone marrow mesenchymal stromal cells from adult Wistar rats
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
0.01, 0.05 and 0.1 µg/mL · in vitroAs given in this study (rat).
Source sentences
Summary
It was observed that MSCs expressed GPR54 receptor to which Kp-10 binds during osteogenic differentiation, promoting a negative effect on osteogenic differentiation.
1 context sentence(design, population, supporting numbers)
Summary · context
Four experimental groups were established in vitro: a control and Kp-10 at concentrations of 0.01, 0.05 and, 0.1 µg/mL.
Report a problemFND-3794878502
03LabIn vitro (cells)Efficacy outcome
In rat bone marrow MSCs, Kp-10 decreased alkaline phosphatase activity at all concentrations in a concentration-dependent manner.
In plain wordsIn rat cells grown in a dish, kisspeptin‑10 lowered an enzyme marker of bone formation, more at higher amounts.
Peptide
Kisspeptin-10
Outcome
DecreaseAlkaline phosphatase activity
Study
ratn: not reported in source
bone marrow mesenchymal stromal cells from adult Wistar rats
Outcome group
Bone and joint · Musculoskeletal
Studied dose
0.01, 0.05 and 0.1 µg/mL · in vitroAs given in this study (rat).
Source sentences
Summary
This effect was observed at all the Kp-10 concentrations in a concentration-dependent manner, characterized by a decrease in the activity of alkaline phosphatase, collagen synthesis, mineralized nodules, and decreased expression of gene transcripts for type I collagen, osteocalcin, osteopontin, and Runx-2.
1 context sentence(design, population, supporting numbers)
Summary · context
Four experimental groups were established in vitro: a control and Kp-10 at concentrations of 0.01, 0.05 and, 0.1 µg/mL.
Report a problemFND-3794878503
04LabIn vitro (cells)Efficacy outcome
In rat bone marrow MSCs, Kp-10 decreased collagen synthesis at all concentrations in a concentration-dependent manner.
In plain wordsIn rat cells grown in a dish, kisspeptin‑10 lowered collagen production, more at higher amounts.
Peptide
Kisspeptin-10
Outcome
DecreaseCollagen synthesis
Study
ratn: not reported in source
bone marrow mesenchymal stromal cells from adult Wistar rats
Outcome group
Bone and joint · Musculoskeletal
Studied dose
0.01, 0.05 and 0.1 µg/mL · in vitroAs given in this study (rat).
Source sentences
Summary
This effect was observed at all the Kp-10 concentrations in a concentration-dependent manner, characterized by a decrease in the activity of alkaline phosphatase, collagen synthesis, mineralized nodules, and decreased expression of gene transcripts for type I collagen, osteocalcin, osteopontin, and Runx-2.
1 context sentence(design, population, supporting numbers)
Summary · context
Four experimental groups were established in vitro: a control and Kp-10 at concentrations of 0.01, 0.05 and, 0.1 µg/mL.
Report a problemFND-3794878504
05LabIn vitro (cells)Efficacy outcome
In rat bone marrow MSCs, Kp-10 decreased mineralized nodules at all concentrations in a concentration-dependent manner.
In plain wordsIn rat cells grown in a dish, kisspeptin‑10 lowered the number of mineral deposits, more at higher amounts.
Peptide
Kisspeptin-10
Outcome
DecreaseMineralized nodules
Study
ratn: not reported in source
bone marrow mesenchymal stromal cells from adult Wistar rats
Outcome group
Bone and joint · Musculoskeletal
Studied dose
0.01, 0.05 and 0.1 µg/mL · in vitroAs given in this study (rat).
Source sentences
Summary
This effect was observed at all the Kp-10 concentrations in a concentration-dependent manner, characterized by a decrease in the activity of alkaline phosphatase, collagen synthesis, mineralized nodules, and decreased expression of gene transcripts for type I collagen, osteocalcin, osteopontin, and Runx-2.
1 context sentence(design, population, supporting numbers)
Summary · context
Four experimental groups were established in vitro: a control and Kp-10 at concentrations of 0.01, 0.05 and, 0.1 µg/mL.
Report a problemFND-3794878505
06LabIn vitro (cells)Mechanism
In rat bone marrow MSCs, Kp-10 decreased expression of type I collagen gene transcripts.
In plain wordsIn rat cells grown in a dish, kisspeptin‑10 lowered the activity of the type I collagen gene.
Peptide
Kisspeptin-10
Outcome
DecreaseType I collagen gene transcripts
Study
ratn: not reported in source
bone marrow mesenchymal stromal cells from adult Wistar rats
Outcome group
Bone and joint · Musculoskeletal
Studied dose
0.01, 0.05 and 0.1 µg/mL · in vitroAs given in this study (rat).
Source sentences
Summary
This effect was observed at all the Kp-10 concentrations in a concentration-dependent manner, characterized by a decrease in the activity of alkaline phosphatase, collagen synthesis, mineralized nodules, and decreased expression of gene transcripts for type I collagen, osteocalcin, osteopontin, and Runx-2.
1 context sentence(design, population, supporting numbers)
Summary · context
Four experimental groups were established in vitro: a control and Kp-10 at concentrations of 0.01, 0.05 and, 0.1 µg/mL.
Report a problemFND-3794878506
07LabIn vitro (cells)Mechanism
In rat bone marrow MSCs, Kp-10 decreased expression of osteocalcin gene transcripts.
In plain wordsIn rat cells grown in a dish, kisspeptin‑10 lowered the activity of the osteocalcin gene.
Peptide
Kisspeptin-10
Outcome
DecreaseOsteocalcin gene transcripts
Study
ratn: not reported in source
bone marrow mesenchymal stromal cells from adult Wistar rats
Outcome group
Bone and joint · Musculoskeletal
Studied dose
0.01, 0.05 and 0.1 µg/mL · in vitroAs given in this study (rat).
Source sentences
Summary
This effect was observed at all the Kp-10 concentrations in a concentration-dependent manner, characterized by a decrease in the activity of alkaline phosphatase, collagen synthesis, mineralized nodules, and decreased expression of gene transcripts for type I collagen, osteocalcin, osteopontin, and Runx-2.
1 context sentence(design, population, supporting numbers)
Summary · context
Four experimental groups were established in vitro: a control and Kp-10 at concentrations of 0.01, 0.05 and, 0.1 µg/mL.
Report a problemFND-3794878507
08LabIn vitro (cells)Mechanism
In rat bone marrow MSCs, Kp-10 decreased expression of osteopontin gene transcripts.
In plain wordsIn rat cells grown in a dish, kisspeptin‑10 lowered the activity of the osteopontin gene.
Peptide
Kisspeptin-10
Outcome
DecreaseOsteopontin gene transcripts
Study
ratn: not reported in source
bone marrow mesenchymal stromal cells from adult Wistar rats
Outcome group
Bone and joint · Musculoskeletal
Studied dose
0.01, 0.05 and 0.1 µg/mL · in vitroAs given in this study (rat).
Source sentences
Summary
This effect was observed at all the Kp-10 concentrations in a concentration-dependent manner, characterized by a decrease in the activity of alkaline phosphatase, collagen synthesis, mineralized nodules, and decreased expression of gene transcripts for type I collagen, osteocalcin, osteopontin, and Runx-2.
1 context sentence(design, population, supporting numbers)
Summary · context
Four experimental groups were established in vitro: a control and Kp-10 at concentrations of 0.01, 0.05 and, 0.1 µg/mL.
Report a problemFND-3794878508
09LabIn vitro (cells)Mechanism
In rat bone marrow MSCs, Kp-10 decreased expression of Runx-2 gene transcripts.
In plain wordsIn rat cells grown in a dish, kisspeptin‑10 lowered the activity of the Runx-2 gene.
Peptide
Kisspeptin-10
Outcome
DecreaseRunx-2 gene transcripts
Study
ratn: not reported in source
bone marrow mesenchymal stromal cells from adult Wistar rats
Outcome group
Bone and joint · Musculoskeletal
Studied dose
0.01, 0.05 and 0.1 µg/mL · in vitroAs given in this study (rat).
Source sentences
Summary
This effect was observed at all the Kp-10 concentrations in a concentration-dependent manner, characterized by a decrease in the activity of alkaline phosphatase, collagen synthesis, mineralized nodules, and decreased expression of gene transcripts for type I collagen, osteocalcin, osteopontin, and Runx-2.
1 context sentence(design, population, supporting numbers)
Summary · context
Four experimental groups were established in vitro: a control and Kp-10 at concentrations of 0.01, 0.05 and, 0.1 µg/mL.
Report a problemFND-3794878509

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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Kisspeptin (Kp-10) inhibits in vitro osteogenic differentiation of multipotent mesenchymal stromal cells extracted from the bone marrow of adult rats.

Summary

Results from this study9

Version history

Subjects

Publication types

MeSH terms

Keywords