The humanin analogue (HNG) alleviates intrauterine adhesions by inhibiting endometrial epithelial cells ferroptosis: a rat model-based study.

Zou L, Huang J, Zhang Q, Mo H, Xia W, Zhu C, Rao M

Human reproduction (Oxford, England)1 Dec 2023Cited by 14 (Europe PMC)

PMID37814907DOI10.1093/humrep/dead196PepBankPUB-37814907

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study9

No result from this study comes from a test of the peptide.

Natural levels measured2

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01HumanEndogenous levelObservational studyMechanism
Endometrial humanin expression was lower in patients with intrauterine adhesions than in controls.
In plain wordsIn women with intrauterine adhesions, the womb lining had lower levels of the body's own humanin than in controls.
Peptide
Humanin
Outcome
DecreaseHumanin expression in endometrium of patients with IUAs
Study
n = 6
Comparator
Controls
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Humanin expression levels were downregulated in the endometria of patients and rats with IUAs relative to the endometria of controls.
1 context sentence(design, population, supporting numbers)
Summary · context
Endometrial tissues from three patients with IUAs and three controls were tested for humanin expression.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3781490701
02AnimalEndogenous levelAnimal study (in vivo)Mechanism
In rats with intrauterine adhesions, endometrial humanin expression was lower than in controls.
In plain wordsIn rats with womb adhesions, the womb lining had lower levels of the body's own humanin than in controls.
Peptide
Humanin
Outcome
DecreaseHumanin (rattin) expression in endometrium of rats with IUAs
Study
ratn: not reported in source
Rat endometrial scraping plus lipopolysaccharide model of intrauterine adhesions
Comparator
Controls
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Humanin expression levels were downregulated in the endometria of patients and rats with IUAs relative to the endometria of controls.
2 context sentences(design, population, supporting numbers)
Summary · context
In the second model, 66 rats were equally randomized to the control, IUA, and IUA + humanin analogue (HNG) groups.
Summary · context
The endometrium was scraped with a surgical spatula, combined with lipopolysaccharide treatment, to establish the rat model of IUAs.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3781490702

Studies that did not test the peptide alone7

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

03AnimalNot a test of Humanin aloneAnimal study (in vivo)Efficacy outcome
In rats with intrauterine adhesions, the humanin analog HNG was followed by a higher mating rate (80%) than in untreated IUA rats (40%).
In plain wordsIn rats with womb adhesions, those given a humanin analog mated more often than untreated rats with adhesions.
Peptide
Humanin
Outcome
IncreaseMating rate with HNG
Study
ratn = 30
Rat endometrial scraping plus lipopolysaccharide model of intrauterine adhesions
Comparator
IUA group without HNG
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Measurements
Mating rate
80%
IUA + HNG
Mating rate
40%
IUA
Source sentences
Summary
The mating rates in the control, IUA, and IUA + HNG groups were 100% (10/10), 40% (4/10), and 80% (8/10), respectively.
2 context sentences(design, population, supporting numbers)
Summary · context
In the second model, 66 rats were equally randomized to the control, IUA, and IUA + humanin analogue (HNG) groups.
Summary · context
The endometrium was scraped with a surgical spatula, combined with lipopolysaccharide treatment, to establish the rat model of IUAs.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3781490703
04AnimalNot a test of Humanin aloneAnimal study (in vivo)Efficacy outcome
In rats with intrauterine adhesions, the humanin analog HNG attenuated the fall in the number of implanted embryos.
In plain wordsIn rats with womb adhesions, a humanin analog limited the drop in the number of embryos.
Peptide
Humanin
Outcome
IncreaseNumber of implanted embryos with HNG
Study
ratn: not reported in source
Rat endometrial scraping plus lipopolysaccharide model of intrauterine adhesions
Comparator
IUA group without HNG
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Measurements
Number of implanted embryos
6.3 mean
IUA + HNG
Source sentences
Summary
HNG supplementation significantly attenuated this decrease in the number of implanted embryos (6.3 ± 4.5) (P < 0.01).
2 context sentences(design, population, supporting numbers)
Summary · context
In the second model, 66 rats were equally randomized to the control, IUA, and IUA + humanin analogue (HNG) groups.
Summary · context
The endometrium was scraped with a surgical spatula, combined with lipopolysaccharide treatment, to establish the rat model of IUAs.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3781490704
05AnimalNot a test of Humanin aloneAnimal study (in vivo)Mechanism
In rats with intrauterine adhesions, the humanin analog HNG attenuated the altered expression of ferroptosis proteins in the endometrium.
In plain wordsIn rats with womb adhesions, a humanin analog reduced changes in proteins linked to a type of cell death.
Peptide
Humanin
Outcome
MixedFerroptosis-related protein expression with HNG
Study
ratn: not reported in source
Rat endometrial scraping plus lipopolysaccharide model of intrauterine adhesions
Comparator
IUA group without HNG
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Source sentences
Summary
Further results showed that HNG significantly attenuated the altered expression levels of proteins involved in ferroptosis in the endometria of rats with IUAs.
2 context sentences(design, population, supporting numbers)
Summary · context
In the second model, 66 rats were equally randomized to the control, IUA, and IUA + humanin analogue (HNG) groups.
Summary · context
The endometrium was scraped with a surgical spatula, combined with lipopolysaccharide treatment, to establish the rat model of IUAs.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3781490705
06AnimalNot a test of Humanin aloneAnimal study (in vivo)Efficacy outcome
In rats with intrauterine adhesions, the humanin analog HNG reduced fibrosis.
In plain wordsIn rats with womb adhesions, a humanin analog reduced scar tissue.
Peptide
Humanin
Outcome
DecreaseEndometrial fibrosis with HNG
Study
ratn: not reported in source
Rat endometrial scraping plus lipopolysaccharide model of intrauterine adhesions
Comparator
IUA group without HNG
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
The findings of this study showed that HNG inhibited ferroptosis and reduced fibrosis in a rat model of IUAs.
2 context sentences(design, population, supporting numbers)
Summary · context
In the second model, 66 rats were equally randomized to the control, IUA, and IUA + humanin analogue (HNG) groups.
Summary · context
The endometrium was scraped with a surgical spatula, combined with lipopolysaccharide treatment, to establish the rat model of IUAs.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3781490706
07LabNot a test of Humanin aloneIn vitro (cells)Efficacy outcome
In Ishikawa cells, the humanin analog HNG attenuated the erastin-induced decrease in cell viability.
In plain wordsIn lab tests, a humanin analog reduced the loss of cell viability caused by erastin.
Peptide
Humanin
Outcome
IncreaseCell viability after erastin with HNG
Study
humann: not reported in source
Ishikawa cell line with erastin-induced ferroptosis
Comparator
Erastin without HNG
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Source sentences
Summary
Moreover, in vitro experiments showed that HNG significantly attenuated the erastin-induced decrease in the viability of the Ishikawa cell line and also attenuated the increase in reactive oxygen species production and the downregulation of GPX4.
1 context sentence(design, population, supporting numbers)
Summary · context
Erastin was used to induce ferroptosis in the Ishikawa cell line.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3781490707
08LabNot a test of Humanin aloneIn vitro (cells)Mechanism
In Ishikawa cells, the humanin analog HNG attenuated the increase in reactive oxygen species production.
In plain wordsIn lab tests, a humanin analog reduced the rise in reactive oxygen species caused by erastin.
Peptide
Humanin
Outcome
DecreaseReactive oxygen species after erastin with HNG
Study
humann: not reported in source
Ishikawa cell line with erastin-induced ferroptosis
Comparator
Erastin without HNG
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Source sentences
Summary
Moreover, in vitro experiments showed that HNG significantly attenuated the erastin-induced decrease in the viability of the Ishikawa cell line and also attenuated the increase in reactive oxygen species production and the downregulation of GPX4.
1 context sentence(design, population, supporting numbers)
Summary · context
Erastin was used to induce ferroptosis in the Ishikawa cell line.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3781490708
09LabNot a test of Humanin aloneIn vitro (cells)Mechanism
In Ishikawa cells, the humanin analog HNG attenuated the erastin-induced downregulation of GPX4.
In plain wordsIn lab tests, a humanin analog reduced the drop in GPX4 caused by erastin.
Peptide
Humanin
Outcome
IncreaseGPX4 expression after erastin with HNG
Study
humann: not reported in source
Ishikawa cell line with erastin-induced ferroptosis
Comparator
Erastin without HNG
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Source sentences
Summary
Moreover, in vitro experiments showed that HNG significantly attenuated the erastin-induced decrease in the viability of the Ishikawa cell line and also attenuated the increase in reactive oxygen species production and the downregulation of GPX4.
1 context sentence(design, population, supporting numbers)
Summary · context
Erastin was used to induce ferroptosis in the Ishikawa cell line.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-3781490709

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.