Kisspeptin treatment reverses high prolactin levels and improves gonadal function in hypothyroid male rats.

Santos LC, Dos Anjos Cordeiro JM, da Silva Santana L, Barbosa EM, Santos BR, Mendonça LD, Cunha MCDSG, Machado WM, Santana LR, Kersul MG, Henriques PC, Lopes RA, Snoeck PPDN, Szawka RE, Silva JF

Scientific reports5 Oct 2023Cited by 9 (Europe PMC)

PMID37798396PMCIDPMC10556046DOI10.1038/s41598-023-44056-zPepBankPUB-37798396

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Summary

We evaluated whether the administration of kisspeptin-10 (Kp10) is capable of restoring gonadal function in hypothyroid male rats. Hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months. In the last month, half of the hypothyroid animals were treated with Kp10. Hypothyroidism reduced testicular and sex gland mass, decreased the proliferation of the seminiferous epithelium, and compromised sperm morphology, motility, and vigor. A decrease in plasma LH and testosterone levels and an increase in prolactin secretion were observed in the hypothyroid rats. Hypothyroidism reduced Kiss1 and Kiss1r protein and gene expression and Star and Cyp11a1 mRNA levels in the testis. Furthermore, it reduced Lhb, Prl, and Drd2 and increased Tshb and Gnrhr expression in the pituitary. In the hypothalamus, hypothyroidism increased Pdyn and Kiss1r while reducing Gnrh1. Kp10 treatment in hypothyroid rats restored testicular and seminal vesicle morphology, improved sperm morphology and motility, reversed high prolactin levels, and increased LH and testosterone levels. In addition, Kp10 increased testicular expression of Kiss1, Kiss1r, Fshr, and Nr5a1 and pituitary Kiss1 expression. Our findings describe the inhibitory effects of hypothyroidism on the male gonadal axis and sperm quality and demonstrate that Kp10 treatment reverses high prolactin levels and improves gonadal function and sperm quality in hypothyroid rats.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study11

11 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In hypothyroid male rats, Kp10 treatment restored testicular and seminal vesicle morphology.
In plain wordsIn male rats with low thyroid function, kisspeptin‑10 restored the structure of the testis and seminal vesicle.
Peptide
Kisspeptin-10
Outcome
Not applicableTesticular and seminal vesicle morphology
Study
ratn: not reported in source
male rats with propylthiouracil-induced hypothyroidism
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated · last month of a three-month hypothyroidism inductionAs given in this study (rat).
Source sentences
Summary
Kp10 treatment in hypothyroid rats restored testicular and seminal vesicle morphology, improved sperm morphology and motility, reversed high prolactin levels, and increased LH and testosterone levels.
2 context sentences(design, population, supporting numbers)
Summary · context
Hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.
Summary · context
In the last month, half of the hypothyroid animals were treated with Kp10.
Show in the study text Report a problemFND-3779839601
02AnimalAnimal study (in vivo)Efficacy outcome
In hypothyroid male rats, Kp10 treatment improved sperm morphology.
In plain wordsIn male rats with low thyroid function, kisspeptin‑10 improved sperm shape.
Peptide
Kisspeptin-10
Outcome
Not applicableSperm morphology
Study
ratn: not reported in source
male rats with propylthiouracil-induced hypothyroidism
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated · last month of a three-month hypothyroidism inductionAs given in this study (rat).
Source sentences
Summary
Kp10 treatment in hypothyroid rats restored testicular and seminal vesicle morphology, improved sperm morphology and motility, reversed high prolactin levels, and increased LH and testosterone levels.
2 context sentences(design, population, supporting numbers)
Summary · context
Hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.
Summary · context
In the last month, half of the hypothyroid animals were treated with Kp10.
Show in the study text Report a problemFND-3779839602
03AnimalAnimal study (in vivo)Efficacy outcome
In hypothyroid male rats, Kp10 treatment improved sperm motility.
In plain wordsIn male rats with low thyroid function, kisspeptin‑10 improved sperm movement.
Peptide
Kisspeptin-10
Outcome
IncreaseSperm motility
Study
ratn: not reported in source
male rats with propylthiouracil-induced hypothyroidism
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated · last month of a three-month hypothyroidism inductionAs given in this study (rat).
Source sentences
Summary
Kp10 treatment in hypothyroid rats restored testicular and seminal vesicle morphology, improved sperm morphology and motility, reversed high prolactin levels, and increased LH and testosterone levels.
2 context sentences(design, population, supporting numbers)
Summary · context
Hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.
Summary · context
In the last month, half of the hypothyroid animals were treated with Kp10.
Show in the study text Report a problemFND-3779839603
04AnimalAnimal study (in vivo)Efficacy outcome
In hypothyroid male rats, Kp10 treatment reversed high prolactin levels.
In plain wordsIn male rats with low thyroid function, kisspeptin‑10 reversed high prolactin levels.
Peptide
Kisspeptin-10
Outcome
DecreasePlasma prolactin
Study
ratn: not reported in source
male rats with propylthiouracil-induced hypothyroidism
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
Dose not stated · last month of a three-month hypothyroidism inductionAs given in this study (rat).
Source sentences
Summary
Kp10 treatment in hypothyroid rats restored testicular and seminal vesicle morphology, improved sperm morphology and motility, reversed high prolactin levels, and increased LH and testosterone levels.
2 context sentences(design, population, supporting numbers)
Summary · context
Hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.
Summary · context
In the last month, half of the hypothyroid animals were treated with Kp10.
Show in the study text Report a problemFND-3779839604
05AnimalAnimal study (in vivo)Efficacy outcome
In hypothyroid male rats, Kp10 treatment increased LH levels.
In plain wordsIn male rats with low thyroid function, kisspeptin‑10 raised luteinizing hormone levels.
Peptide
Kisspeptin-10
Outcome
IncreasePlasma LH
Study
ratn: not reported in source
male rats with propylthiouracil-induced hypothyroidism
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
Dose not stated · last month of a three-month hypothyroidism inductionAs given in this study (rat).
Source sentences
Summary
Kp10 treatment in hypothyroid rats restored testicular and seminal vesicle morphology, improved sperm morphology and motility, reversed high prolactin levels, and increased LH and testosterone levels.
2 context sentences(design, population, supporting numbers)
Summary · context
Hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.
Summary · context
In the last month, half of the hypothyroid animals were treated with Kp10.
Show in the study text Report a problemFND-3779839605
06AnimalAnimal study (in vivo)Efficacy outcome
In hypothyroid male rats, Kp10 treatment increased testosterone levels.
In plain wordsIn male rats with low thyroid function, kisspeptin‑10 raised testosterone levels.
Peptide
Kisspeptin-10
Outcome
IncreasePlasma testosterone
Study
ratn: not reported in source
male rats with propylthiouracil-induced hypothyroidism
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
Dose not stated · last month of a three-month hypothyroidism inductionAs given in this study (rat).
Source sentences
Summary
Kp10 treatment in hypothyroid rats restored testicular and seminal vesicle morphology, improved sperm morphology and motility, reversed high prolactin levels, and increased LH and testosterone levels.
2 context sentences(design, population, supporting numbers)
Summary · context
Hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.
Summary · context
In the last month, half of the hypothyroid animals were treated with Kp10.
Show in the study text Report a problemFND-3779839606
07AnimalAnimal study (in vivo)Mechanism
In hypothyroid male rats, Kp10 increased testicular expression of Kiss1.
In plain wordsIn male rats with low thyroid function, kisspeptin‑10 raised testis activity of the Kiss1 gene.
Peptide
Kisspeptin-10
Outcome
IncreaseTesticular Kiss1 expression
Study
ratn: not reported in source
male rats with propylthiouracil-induced hypothyroidism
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated · last month of a three-month hypothyroidism inductionAs given in this study (rat).
Source sentences
Summary
In addition, Kp10 increased testicular expression of Kiss1, Kiss1r, Fshr, and Nr5a1 and pituitary Kiss1 expression.
2 context sentences(design, population, supporting numbers)
Summary · context
Hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.
Summary · context
In the last month, half of the hypothyroid animals were treated with Kp10.
Show in the study text Report a problemFND-3779839607
08AnimalAnimal study (in vivo)Mechanism
In hypothyroid male rats, Kp10 increased testicular expression of Kiss1r.
In plain wordsIn male rats with low thyroid function, kisspeptin‑10 raised testis activity of the Kiss1r gene.
Peptide
Kisspeptin-10
Outcome
IncreaseTesticular Kiss1r expression
Study
ratn: not reported in source
male rats with propylthiouracil-induced hypothyroidism
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated · last month of a three-month hypothyroidism inductionAs given in this study (rat).
Source sentences
Summary
In addition, Kp10 increased testicular expression of Kiss1, Kiss1r, Fshr, and Nr5a1 and pituitary Kiss1 expression.
2 context sentences(design, population, supporting numbers)
Summary · context
Hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.
Summary · context
In the last month, half of the hypothyroid animals were treated with Kp10.
Show in the study text Report a problemFND-3779839608
09AnimalAnimal study (in vivo)Mechanism
In hypothyroid male rats, Kp10 increased testicular expression of Fshr.
In plain wordsIn male rats with low thyroid function, kisspeptin‑10 raised testis activity of the Fshr gene.
Peptide
Kisspeptin-10
Outcome
IncreaseTesticular Fshr expression
Study
ratn: not reported in source
male rats with propylthiouracil-induced hypothyroidism
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated · last month of a three-month hypothyroidism inductionAs given in this study (rat).
Source sentences
Summary
In addition, Kp10 increased testicular expression of Kiss1, Kiss1r, Fshr, and Nr5a1 and pituitary Kiss1 expression.
2 context sentences(design, population, supporting numbers)
Summary · context
Hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.
Summary · context
In the last month, half of the hypothyroid animals were treated with Kp10.
Show in the study text Report a problemFND-3779839609
10AnimalAnimal study (in vivo)Mechanism
In hypothyroid male rats, Kp10 increased testicular expression of Nr5a1.
In plain wordsIn male rats with low thyroid function, kisspeptin‑10 raised testis activity of the Nr5a1 gene.
Peptide
Kisspeptin-10
Outcome
IncreaseTesticular Nr5a1 expression
Study
ratn: not reported in source
male rats with propylthiouracil-induced hypothyroidism
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated · last month of a three-month hypothyroidism inductionAs given in this study (rat).
Source sentences
Summary
In addition, Kp10 increased testicular expression of Kiss1, Kiss1r, Fshr, and Nr5a1 and pituitary Kiss1 expression.
2 context sentences(design, population, supporting numbers)
Summary · context
Hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.
Summary · context
In the last month, half of the hypothyroid animals were treated with Kp10.
Show in the study text Report a problemFND-3779839610
11AnimalAnimal study (in vivo)Mechanism
In hypothyroid male rats, Kp10 increased pituitary expression of Kiss1.
In plain wordsIn male rats with low thyroid function, kisspeptin‑10 raised pituitary activity of the Kiss1 gene.
Peptide
Kisspeptin-10
Outcome
IncreasePituitary Kiss1 expression
Study
ratn: not reported in source
male rats with propylthiouracil-induced hypothyroidism
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated · last month of a three-month hypothyroidism inductionAs given in this study (rat).
Source sentences
Summary
In addition, Kp10 increased testicular expression of Kiss1, Kiss1r, Fshr, and Nr5a1 and pituitary Kiss1 expression.
2 context sentences(design, population, supporting numbers)
Summary · context
Hypothyroidism was induced with 6-propyl-2-thiouracil (PTU) for three months.
Summary · context
In the last month, half of the hypothyroid animals were treated with Kp10.
Show in the study text Report a problemFND-3779839611

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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