β-nicotinamide mononucleotide rescues the quality of aged oocyte and improves subsequent embryo development in pigs.

Li L, Han Q, Chen Y, Zhang M, Wang L, An X, Zhang S, Zhai Y, Dai X, Tang B, Li Z, Xie G

PloS one5 Oct 2023Cited by 14 (Europe PMC)

PMID37796824PMCIDPMC10553265DOI10.1371/journal.pone.0291640PepBankPUB-37796824

Summary

Oocyte senescence alters the shape and function, thereby weakening the fertilization potential. Nicotinamide mononucleotide (NMN) reverses age-related dysfunctions in various organs. Studies had shown long-term administration of NMN reduced the physiological decline associated in aged mice and reversed the aging of the ovaries. However, the protective effect of NMN on aged porcine oocytes is still unclear. In this study, we investigated the effects of NMN on aging porcine oocytes and subsequent embryonic development. We established a model of senescence of porcine oocytes after ovulation by extending the culture time in vitro. NMN supplementation significantly reduced reactive oxygen species (ROS) levels in senescence oocytes and increased the mRNA levels of antioxidant genes SOD1 and Cat. The mitochondrial membrane potential of aged oocytes treated with NMN was increased compared with that of untreated oocytes. In addition, the mRNA level of apoptosis-related gene Bax was significantly decreased in senescence oocytes treated with NMN, while the mRNA level of anti-apoptosis-related gene BCL-2 was significantly increased. Furthermore, NMN supplementation enhanced the subsequent development ability of senescent oocytes during in vitro aging. Compared with untreated senescent oocytes, the blastocyst formation rate and pluripotent genes of senescent oocytes treated with NMN were significantly increased. Taken together, these results suggest that NMN is beneficial for delaying the aging process in porcine oocytes.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study9

9 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In senescent porcine oocytes, NMN reduced reactive oxygen species levels compared with untreated senescent oocytes.
In plain wordsIn lab tests, NMN reduced reactive oxygen species levels in aged pig oocytes compared with untreated aged oocytes.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseReactive oxygen species
Study
pign: not reported in source
porcine oocytes aged in vitro
Comparator
Untreated senescent oocytes
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
NMN supplementation significantly reduced reactive oxygen species (ROS) levels in senescence oocytes and increased the mRNA levels of antioxidant genes SOD1 and Cat.
1 context sentence(design, population, supporting numbers)
Summary · context
We established a model of senescence of porcine oocytes after ovulation by extending the culture time in vitro.
Show in the study text Report a problemFND-3779682401
02LabIn vitro (cells)Mechanism
In senescent porcine oocytes, NMN increased SOD1 mRNA levels compared with untreated senescent oocytes.
In plain wordsIn lab tests, NMN increased SOD1 gene expression in aged pig oocytes compared with untreated aged oocytes.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseSOD1 mRNA
Study
pign: not reported in source
porcine oocytes aged in vitro
Comparator
Untreated senescent oocytes
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
NMN supplementation significantly reduced reactive oxygen species (ROS) levels in senescence oocytes and increased the mRNA levels of antioxidant genes SOD1 and Cat.
1 context sentence(design, population, supporting numbers)
Summary · context
We established a model of senescence of porcine oocytes after ovulation by extending the culture time in vitro.
Show in the study text Report a problemFND-3779682402
03LabIn vitro (cells)Mechanism
In senescent porcine oocytes, NMN increased Cat mRNA levels compared with untreated senescent oocytes.
In plain wordsIn lab tests, NMN increased Cat gene expression in aged pig oocytes compared with untreated aged oocytes.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseCat mRNA
Study
pign: not reported in source
porcine oocytes aged in vitro
Comparator
Untreated senescent oocytes
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
NMN supplementation significantly reduced reactive oxygen species (ROS) levels in senescence oocytes and increased the mRNA levels of antioxidant genes SOD1 and Cat.
1 context sentence(design, population, supporting numbers)
Summary · context
We established a model of senescence of porcine oocytes after ovulation by extending the culture time in vitro.
Show in the study text Report a problemFND-3779682403
04LabIn vitro (cells)Mechanism
In aged porcine oocytes, NMN increased mitochondrial membrane potential compared with untreated oocytes.
In plain wordsIn lab tests on aged pig oocytes, NMN increased mitochondrial membrane potential compared with untreated oocytes.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseMitochondrial membrane potential
Study
pign: not reported in source
porcine oocytes aged in vitro
Comparator
Untreated senescent oocytes
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
The mitochondrial membrane potential of aged oocytes treated with NMN was increased compared with that of untreated oocytes.
1 context sentence(design, population, supporting numbers)
Summary · context
We established a model of senescence of porcine oocytes after ovulation by extending the culture time in vitro.
Show in the study text Report a problemFND-3779682404
05LabIn vitro (cells)Mechanism
In senescent porcine oocytes, NMN decreased Bax mRNA levels compared with untreated senescent oocytes.
In plain wordsIn lab tests, NMN reduced Bax gene expression in aged pig oocytes compared with untreated aged oocytes.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseBax mRNA
Study
pign: not reported in source
porcine oocytes aged in vitro
Comparator
Untreated senescent oocytes
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
In addition, the mRNA level of apoptosis-related gene Bax was significantly decreased in senescence oocytes treated with NMN, while the mRNA level of anti-apoptosis-related gene BCL-2 was significantly increased.
1 context sentence(design, population, supporting numbers)
Summary · context
We established a model of senescence of porcine oocytes after ovulation by extending the culture time in vitro.
Show in the study text Report a problemFND-3779682405
06LabIn vitro (cells)Mechanism
In senescent porcine oocytes, NMN increased BCL-2 mRNA levels compared with untreated senescent oocytes.
In plain wordsIn lab tests, NMN increased BCL-2 gene expression in aged pig oocytes compared with untreated aged oocytes.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseBCL-2 mRNA
Study
pign: not reported in source
porcine oocytes aged in vitro
Comparator
Untreated senescent oocytes
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
In addition, the mRNA level of apoptosis-related gene Bax was significantly decreased in senescence oocytes treated with NMN, while the mRNA level of anti-apoptosis-related gene BCL-2 was significantly increased.
1 context sentence(design, population, supporting numbers)
Summary · context
We established a model of senescence of porcine oocytes after ovulation by extending the culture time in vitro.
Show in the study text Report a problemFND-3779682406
07LabIn vitro (cells)Efficacy outcome
In senescent porcine oocytes, NMN enhanced subsequent developmental ability compared with untreated senescent oocytes.
In plain wordsIn lab tests, NMN improved subsequent development in aged pig oocytes compared with untreated aged oocytes.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseSubsequent developmental ability
Study
pign: not reported in source
porcine oocytes aged in vitro
Comparator
Untreated senescent oocytes
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, NMN supplementation enhanced the subsequent development ability of senescent oocytes during in vitro aging.
1 context sentence(design, population, supporting numbers)
Summary · context
We established a model of senescence of porcine oocytes after ovulation by extending the culture time in vitro.
Show in the study text Report a problemFND-3779682407
08LabIn vitro (cells)Efficacy outcome
In senescent porcine oocytes, NMN significantly increased blastocyst formation compared with untreated senescent oocytes.
In plain wordsIn lab tests on aged pig oocytes, NMN increased blastocyst formation compared with untreated aged oocytes.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseBlastocyst formation rate
Study
pign: not reported in source
porcine oocytes aged in vitro
Comparator
Untreated senescent oocytes
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Compared with untreated senescent oocytes, the blastocyst formation rate and pluripotent genes of senescent oocytes treated with NMN were significantly increased.
1 context sentence(design, population, supporting numbers)
Summary · context
We established a model of senescence of porcine oocytes after ovulation by extending the culture time in vitro.
Show in the study text Report a problemFND-3779682408
09LabIn vitro (cells)Mechanism
In senescent porcine oocytes, NMN significantly increased pluripotent genes compared with untreated senescent oocytes.
In plain wordsIn lab tests on aged pig oocytes, NMN increased pluripotent genes compared with untreated aged oocytes.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreasePluripotent genes
Study
pign: not reported in source
porcine oocytes aged in vitro
Comparator
Untreated senescent oocytes
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Compared with untreated senescent oocytes, the blastocyst formation rate and pluripotent genes of senescent oocytes treated with NMN were significantly increased.
1 context sentence(design, population, supporting numbers)
Summary · context
We established a model of senescence of porcine oocytes after ovulation by extending the culture time in vitro.
Show in the study text Report a problemFND-3779682409

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

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