Lesions of KNDy and Kiss1R Neurons in the Arcuate Nucleus Produce Different Effects on LH Pulse Patterns in Female Sheep.

Goodman RL, Moore AM, Onslow K, Hileman SM, Hardy SL, Bowdridge EC, Walters BA, Agus S, Griesgraber MJ, Aerts EG, Lehman MN, Coolen LM

Endocrinology1 Sept 2023Cited by 17 (Europe PMC)

PMID37776515PMCIDPMC10587900DOI10.1210/endocr/bqad148PepBankPUB-37776515

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone7

In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Kisspeptin-54 (metastin).

01AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In ewes, Kiss-SAP (saporin conjugated to kisspeptin‑54) lesioned about two-thirds of the Kiss1R cell population in the arcuate nucleus.
In plain wordsIn sheep, a kisspeptin‑54 toxin conjugate destroyed about two-thirds of the cells that carry the kisspeptin receptor.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreaseKiss1R cell population lesioned by Kiss-SAP
Study
sheepn: not reported in source
female sheep (ewes), arcuate nucleus lesions
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Kiss-SAP (saporin conjugated to kisspeptin-54) lesioned about two-thirds of the Kiss1R population without affecting KNDy or GnRH cell number.
1 context sentence(design, population, supporting numbers)
Summary · context
This study tested the functional role of ovine KNDy neurons in pulse generation and explored the roles of nearby Kiss1 receptor (Kiss1R)-containing cells using lesions produced with saporin (SAP) conjugates.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3777651501
02AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In ewes, Kiss-SAP lesions did not change KNDy or GnRH cell number.
In plain wordsIn sheep, the kisspeptin‑54 toxin conjugate did not change the number of KNDy or GnRH cells.
Peptide
Kisspeptin-54 (metastin)
Outcome
No significant changeKNDy and GnRH cell number after Kiss-SAP
Study
sheepn: not reported in source
female sheep (ewes), arcuate nucleus lesions
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
without affecting KNDy or GnRH cell number.
1 context sentence(design, population, supporting numbers)
Summary · context
This study tested the functional role of ovine KNDy neurons in pulse generation and explored the roles of nearby Kiss1 receptor (Kiss1R)-containing cells using lesions produced with saporin (SAP) conjugates.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3777651502
03AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Efficacy outcome
In ewes, Kiss-SAP lesions produced a dramatic decrease in LH pulse amplitude.
In plain wordsIn sheep, the kisspeptin‑54 toxin conjugate sharply lowered the size of LH pulses.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreaseLH pulse amplitude
Study
sheepn: not reported in source
female sheep (ewes), arcuate nucleus lesions
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Both lesions produced a dramatic decrease in LH pulse amplitude but had different effects on LH pulse patterns.
1 context sentence(design, population, supporting numbers)
Summary · context
This study tested the functional role of ovine KNDy neurons in pulse generation and explored the roles of nearby Kiss1 receptor (Kiss1R)-containing cells using lesions produced with saporin (SAP) conjugates.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3777651503
04AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Efficacy outcome
In ewes, Kiss-SAP did not increase the LH interpulse interval.
In plain wordsIn sheep, the kisspeptin‑54 toxin conjugate did not change the time between LH pulses.
Peptide
Kisspeptin-54 (metastin)
Outcome
No significant changeLH interpulse interval
Study
sheepn: not reported in source
female sheep (ewes), arcuate nucleus lesions
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
NK3-SAP increased interpulse interval, but Kiss-SAP did not.
1 context sentence(design, population, supporting numbers)
Summary · context
This study tested the functional role of ovine KNDy neurons in pulse generation and explored the roles of nearby Kiss1 receptor (Kiss1R)-containing cells using lesions produced with saporin (SAP) conjugates.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3777651504
05AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Efficacy outcome
In ewes, Kiss-SAP disrupted the regular hourly occurrence of LH pulses.
In plain wordsIn sheep, the kisspeptin‑54 toxin conjugate disrupted the regular hourly rhythm of LH pulses.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreaseRegularity of hourly LH pulses
Study
sheepn: not reported in source
female sheep (ewes), arcuate nucleus lesions
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
In contrast, Kiss-SAP disrupted the regular hourly occurrence of LH pulses, but NK3-SAP did not.
1 context sentence(design, population, supporting numbers)
Summary · context
This study tested the functional role of ovine KNDy neurons in pulse generation and explored the roles of nearby Kiss1 receptor (Kiss1R)-containing cells using lesions produced with saporin (SAP) conjugates.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3777651505
06AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In ewes, 68% of arcuate Kiss1R-containing cells contained transcript markers for GABA.
In plain wordsIn sheep, 68% of arcuate cells carrying the kisspeptin receptor had markers for GABA.
Peptide
Kisspeptin-54 (metastin)
Outcome
Not applicableGABA transcript in Kiss1R-containing arcuate cells
Study
sheepn: not reported in source
female sheep (ewes), arcuate nucleus lesions
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Measurements
Kiss1R cells containing GABA transcript
68%
arcuate Kiss1R cells
Source sentences
Summary
Kiss1R cells primarily contained transcript markers for GABA (68%), glutamate (28%), ESR1 (estrogen receptor-α) mRNA, and OPRK1 (kappa opioid receptor) mRNA.
1 context sentence(design, population, supporting numbers)
Summary · context
This study tested the functional role of ovine KNDy neurons in pulse generation and explored the roles of nearby Kiss1 receptor (Kiss1R)-containing cells using lesions produced with saporin (SAP) conjugates.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3777651506
07AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In ewes, 28% of arcuate Kiss1R-containing cells contained transcript markers for glutamate.
In plain wordsIn sheep, 28% of arcuate cells carrying the kisspeptin receptor had markers for glutamate.
Peptide
Kisspeptin-54 (metastin)
Outcome
Not applicableGlutamate transcript in Kiss1R-containing arcuate cells
Study
sheepn: not reported in source
female sheep (ewes), arcuate nucleus lesions
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Measurements
Kiss1R cells containing glutamate transcript
28%
arcuate Kiss1R cells
Source sentences
Summary
Kiss1R cells primarily contained transcript markers for GABA (68%), glutamate (28%), ESR1 (estrogen receptor-α) mRNA, and OPRK1 (kappa opioid receptor) mRNA.
1 context sentence(design, population, supporting numbers)
Summary · context
This study tested the functional role of ovine KNDy neurons in pulse generation and explored the roles of nearby Kiss1 receptor (Kiss1R)-containing cells using lesions produced with saporin (SAP) conjugates.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3777651507

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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