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Study · Ex vivo tissue · 2023

4 results

Exogenous humanin and MOTS-c function as protective agents against gentamicin-induced hair cell damage.

Waldmann D, Lu Y, Cortada M, Bodmer D, Levano Huaman S

Biochemical and biophysical research communications17 Aug 2023Cited by 3 (Europe PMC)

PMID37633181 (opens in a new tab)DOI10.1016/j.bbrc.2023.08.033 (opens in a new tab)PepBankPUB-37633181
Publisher pagePubMedAsk about this study
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Summary

Loss of hair cells can lead to irreversible sensorineural hearing loss. Therefore, hair cell preservation is critical for hearing. Mitochondrial derived peptides (MDPs) are bioactive peptides and prominent members of this family are humanin (HN) and the mitochondrial-open-reading frame of the twelve S c (MOTS-c). The protective roles of HN and MOTS-c in age-related diseases and in various tissues exposed to cellular stresses have been demonstrated. The involvement of MDPs in the inner ear remains to be investigated. Therefore, we determined the expression of rattin, the homolog of humanin, in inner ear tissues. Then, we found that HN and MOTS-c showed a significant protective effect on hair cells in organ of Corti explants exposed to gentamicin. Treatment with HN decreased gentamicin-induced phosphorylation of AKT, whereas treatment with MOTS-c increased phosphorylation of AMPKα in explants. Our data indicate that MDPs exert a protective function in gentamicin-induced hair cell damage. Therefore, MDPs may contribute to design new preventive strategies against hearing loss.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueEfficacy outcome

    In organ of Corti explants exposed to gentamicin, MOTS‑c (and humanin) had a significant protective effect on hair cells.

    In plain wordsIn lab tests on inner-ear tissue, MOTS‑c protected hair cells from damage by the antibiotic gentamicin.

    Peptide
    MOTS-c
    Outcome
    IncreaseHair cell protection against gentamicin damage
    Study

    n: not reported in source

    Organ of Corti explants exposed to gentamicin
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Then, we found that HN and MOTS-c showed a significant protective effect on hair cells in organ of Corti explants exposed to gentamicin.
  2. 02LabEx vivo tissueMechanism

    In organ of Corti explants, treatment with MOTS‑c increased phosphorylation of AMPKα.

    In plain wordsIn lab tests on inner-ear tissue, MOTS‑c increased the activation of AMPKα, a cell energy signal.

    Peptide
    MOTS-c
    Outcome
    IncreaseAMPKα phosphorylation
    Study

    n: not reported in source

    Organ of Corti explants exposed to gentamicin
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    whereas treatment with MOTS-c increased phosphorylation of AMPKα in explants.
    1 context sentence
    Summary · context
    Then, we found that HN and MOTS-c showed a significant protective effect on hair cells in organ of Corti explants exposed to gentamicin.
  3. 03LabEx vivo tissueEfficacy outcome

    In organ of Corti explants exposed to gentamicin, humanin had a significant protective effect on hair cells.

    In plain wordsIn lab tests on ear tissue, humanin protected hair cells from damage by the antibiotic gentamicin.

    Peptide
    Humanin
    Outcome
    IncreaseHair cell protection against gentamicin
    Study

    n: not reported in source

    Organ of Corti explants exposed to gentamicin
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Then, we found that HN and MOTS-c showed a significant protective effect on hair cells in organ of Corti explants exposed to gentamicin.
  4. 04LabEx vivo tissueMechanism

    In organ of Corti explants, humanin treatment decreased gentamicin-induced phosphorylation of AKT.

    In plain wordsIn lab tests on ear tissue, humanin lowered the gentamicin-induced rise in AKT phosphorylation.

    Peptide
    Humanin
    Outcome
    DecreaseGentamicin-induced AKT phosphorylation
    Study

    n: not reported in source

    Organ of Corti explants exposed to gentamicin
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Treatment with HN decreased gentamicin-induced phosphorylation of AKT, whereas treatment with MOTS-c increased phosphorylation of AMPKα in explants.
    1 context sentence
    Summary · context
    Then, we found that HN and MOTS-c showed a significant protective effect on hair cells in organ of Corti explants exposed to gentamicin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Versions 4 to 18Current

    28 Oct 2026, 02:02 UTC

    The same notice was added 15 times, first on 13 Oct 2026, 21:44 UTC. The status did not change between them.

    Study results updated

  2. Version 3

    13 Oct 2026, 16:31 UTC

    Study data updated · source update from Europe PMC

  3. Version 2

    10 Oct 2026, 08:19 UTC

    Study results updated

  4. Version 1

    9 Oct 2026, 02:47 UTC

    First added · source update from Europe PMC

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