Nicotinamide mononucleotide (NMN) alleviates the poly(I:C)-induced inflammatory response in human primary cell cultures.

Sano H, Kratz A, Nishino T, Imamura H, Yoshida Y, Shimizu N, Kitano H, Yachie A

Scientific reports20 Jul 2023Cited by 6 (Europe PMC)

PMID37474783PMCIDPMC10359400DOI10.1038/s41598-023-38762-xPepBankPUB-37474783

Summary

NMN is the direct precursor of nicotinamide adenine dinucleotide (NAD+) and is considered as a key factor for increasing NAD+ levels and mitochondrial activity in cells. In this study, based on transcriptome analysis, we showed that NMN alleviates the poly(I:C)-induced inflammatory response in cultures of two types of human primary cells, human pulmonary microvascular endothelial cells (HPMECs) and human coronary artery endothelial cells (HCAECs). Major inflammatory mediators, including IL6 and PARP family members, were grouped into coexpressed gene modules and significantly downregulated under NMN exposure in poly(I:C)-activated conditions in both cell types. The Bayesian network analysis of module hub genes predicted common genes, including eukaryotic translation initiation factor 4B (EIF4B), and distinct genes, such as platelet-derived growth factor binding molecules, in HCAECs, which potentially regulate the identified inflammation modules. These results suggest a robust regulatory mechanism by which NMN alleviates inflammatory pathway activation, which may open up the possibility of a new role for NMN replenishment in the treatment of chronic or acute inflammation.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

3 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In human pulmonary microvascular and coronary artery endothelial cell cultures, NMN alleviated the poly(I:C)-induced inflammatory response.
In plain wordsIn human cells, NMN reduced the inflammatory response induced by poly(I:C).
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseInflammatory response
Study
humann: not reported in source
poly(I:C)-activated HPMECs and HCAECs
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
Dose not stated
Source sentences
Summary
In this study, based on transcriptome analysis, we showed that NMN alleviates the poly(I:C)-induced inflammatory response in cultures of two types of human primary cells, human pulmonary microvascular endothelial cells (HPMECs) and human coronary artery endothelial cells (HCAECs).
Show in the study text Report a problemFND-3747478301
02LabIn vitro (cells)Mechanism
In both human endothelial cell types, NMN significantly downregulated IL6 under poly(I:C)-activated conditions.
In plain wordsIn human cells activated by poly(I:C), NMN reduced IL6 expression.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseIL6 expression
Study
humann: not reported in source
poly(I:C)-activated HPMECs and HCAECs
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
Dose not stated
Source sentences
Summary
Major inflammatory mediators, including IL6 and PARP family members, were grouped into coexpressed gene modules and significantly downregulated under NMN exposure in poly(I:C)-activated conditions in both cell types.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, based on transcriptome analysis, we showed that NMN alleviates the poly(I:C)-induced inflammatory response in cultures of two types of human primary cells, human pulmonary microvascular endothelial cells (HPMECs) and human coronary artery endothelial cells (HCAECs).
Show in the study text Report a problemFND-3747478302
03LabIn vitro (cells)Mechanism
In both human endothelial cell types, NMN significantly downregulated PARP family members under poly(I:C)-activated conditions.
In plain wordsIn human cells activated by poly(I:C), NMN reduced PARP family expression.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreasePARP family expression
Study
humann: not reported in source
poly(I:C)-activated HPMECs and HCAECs
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
Dose not stated
Source sentences
Summary
Major inflammatory mediators, including IL6 and PARP family members, were grouped into coexpressed gene modules and significantly downregulated under NMN exposure in poly(I:C)-activated conditions in both cell types.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, based on transcriptome analysis, we showed that NMN alleviates the poly(I:C)-induced inflammatory response in cultures of two types of human primary cells, human pulmonary microvascular endothelial cells (HPMECs) and human coronary artery endothelial cells (HCAECs).
Show in the study text Report a problemFND-3747478303

Studies that did not test the peptide alone1

In this study, NMN (nicotinamide mononucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking NMN (nicotinamide mononucleotide).

04Computer modelNot a test of NMN (nicotinamide mononucleotide) aloneIn silicoMechanism
Bayesian network analysis predicted common and distinct potential regulators of NMN-associated inflammation modules, including EIF4B and platelet-derived growth factor binding molecules.
In plain wordsComputer analysis predicted shared and distinct potential regulators of NMN-associated inflammation modules.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Not applicablePredicted inflammation module regulators
Study
humann: not reported in source
Bayesian network analysis of module hub genes
Outcome group
Inflammation markers · Immune and inflammation
Source sentences
Summary
The Bayesian network analysis of module hub genes predicted common genes, including eukaryotic translation initiation factor 4B (EIF4B), and distinct genes, such as platelet-derived growth factor binding molecules, in HCAECs, which potentially regulate the identified inflammation modules.
In this study, NMN (nicotinamide mononucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NMN (nicotinamide mononucleotide).
Show in the study text Report a problemFND-3747478304

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

MeSH terms

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