Assessing the Effects of Nicotinamide Mononucleotide Supplementation on Pulmonary Inflammation in Male Mice Subchronically Exposed to Ambient Particulate Matter.

Zhang R, Chen S, Wang Z, Ye L, Jiang Y, Li M, Jiang X, Peng H, Guo Z, Chen L, Zhang R, Niu Y, Aschner M, Li D, Chen W

Environmental health perspectives17 Jul 2023Cited by 16 (Europe PMC)

PMID37458712PMCIDPMC10351503DOI10.1289/ehp12259PepBankPUB-37458712

Summary

BackgroundChronic lung injury and dysregulated cellular homeostasis in response to particulate matter (PM) exposure are closely associated with adverse health effects. However, an effective intervention for preventing the adverse health effects has not been developed.ObjectivesThis study aimed to evaluate the protective effects of nicotinamide mononucleotide (NMN) supplementation on lung injury and elucidate the mechanism by which NMN improved immune function following subchronic PM exposure.MethodsSix-week-old male C57BL/6J mice were placed in a real-ambient PM exposure system or filtered air-equipped chambers (control) for 16 wk with or without NMN supplementation in drinking water (regarded as Con-H2O, Exp-H2O, Con-NMN and Exp-NMN groups, respectively) in Shijiazhuang City, China (n=20/group). The effects of NMN supplementation (500mg/kg) on PM-induced chronic pulmonary inflammation were assessed, and its mechanism was characterized using single-cell transcriptomic sequencing (scRNA-seq) analysis of whole lung cells.ResultsThe NMN-treated mice exhibited higher NAD+ levels in multiple tissues. Following 16-wk PM exposure, slightly less pulmonary inflammation and less collagen deposition were noted in mice with NMN supplementation in response to real-ambient PM exposure (Exp-NMN group) compared with the Exp-H2O group (all p<0.05). Mouse lung tissue isolated from the Exp-NMN group was characterized by fewer neutrophils, monocyte-derived cells, fibroblasts, and myeloid-derived suppressor cells induced by subchronic PM exposure as detected by scRNA-seq transcriptomic analysis. The improved immune functions were further characterized by interleukin-17 signaling pathway inhibition and lower secretion of profibrotic cytokines in the Exp-NMN group compared with the Exp-H2O group. In addition, reduced proportions of differentiated myofibroblasts and profibrotic interstitial macrophages were identified in the NMN-supplemented mice in response to PM exposure. Furthermore, less immune function suppression and altered differentiation of pathological cell phenotypes NMN was related to intracellular lipid metabolism activation.DiscussionOur novel findings suggest that NMN supplementation mitigated PM-induced lung injury by regulating immune functions and improving lipid metabolism in male mice, providing a putative intervention method for prevention of human health effects associated with PM exposure. https://doi.org/10.1289/EHP12259.

CC0Text from Europe PMC. Highlighted sentences are the source of results.

Results from this study12

12 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Mechanism
In mice, NMN treatment increased NAD+ levels in multiple tissues.
In plain wordsIn mice, NMN increased NAD+ levels in several tissues.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseTissue NAD+
Study
mousen: not reported in source
male C57BL/6J mice exposed to ambient particulate matter
Outcome group
Mitochondrial function · Whole body
Studied dose
500 mg/kg · drinking water · 16 weeksAs given in this study (mouse).
Source sentences
Summary
The NMN-treated mice exhibited higher NAD+ levels in multiple tissues.
2 context sentences(design, population, supporting numbers)
Summary · context
Six-week-old male C57BL/6J mice were placed in a real-ambient PM exposure system or filtered air-equipped chambers (control) for 16 wk with or without NMN supplementation in drinking water (regarded as Con-H2O, Exp-H2O, Con-NMN and Exp-NMN groups, respectively) in Shijiazhuang City, China (n=20/group).
Summary · context
The effects of NMN supplementation (500mg/kg) on PM-induced chronic pulmonary inflammation were assessed, and its mechanism was characterized using single-cell transcriptomic sequencing (scRNA-seq) analysis of whole lung cells.
Show in the study text Report a problemFND-3745871201
02AnimalAnimal study (in vivo)Efficacy outcome
In PM-exposed mice, NMN slightly reduced pulmonary inflammation compared with exposure without NMN.
In plain wordsIn mice exposed to particulate matter, NMN slightly reduced lung inflammation compared with exposure without NMN.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreasePulmonary inflammation
Study
mousen: not reported in source
male C57BL/6J mice exposed to ambient particulate matter
Comparator
PM-exposed mice without NMN
Outcome group
Immune response · Respiratory
Studied dose
500 mg/kg · drinking water · 16 weeksAs given in this study (mouse).
Source sentences
Summary
Following 16-wk PM exposure, slightly less pulmonary inflammation and less collagen deposition were noted in mice with NMN supplementation in response to real-ambient PM exposure (Exp-NMN group) compared with the Exp-H2O group (all p<0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
Six-week-old male C57BL/6J mice were placed in a real-ambient PM exposure system or filtered air-equipped chambers (control) for 16 wk with or without NMN supplementation in drinking water (regarded as Con-H2O, Exp-H2O, Con-NMN and Exp-NMN groups, respectively) in Shijiazhuang City, China (n=20/group).
Summary · context
The effects of NMN supplementation (500mg/kg) on PM-induced chronic pulmonary inflammation were assessed, and its mechanism was characterized using single-cell transcriptomic sequencing (scRNA-seq) analysis of whole lung cells.
Show in the study text Report a problemFND-3745871202
03AnimalAnimal study (in vivo)Efficacy outcome
In PM-exposed mice, NMN reduced pulmonary collagen deposition compared with exposure without NMN.
In plain wordsIn mice exposed to particulate matter, NMN reduced lung collagen deposits compared with exposure without NMN.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseCollagen deposition
Study
mousen: not reported in source
male C57BL/6J mice exposed to ambient particulate matter
Comparator
PM-exposed mice without NMN
Outcome group
Immune response · Respiratory
Studied dose
500 mg/kg · drinking water · 16 weeksAs given in this study (mouse).
Source sentences
Summary
Following 16-wk PM exposure, slightly less pulmonary inflammation and less collagen deposition were noted in mice with NMN supplementation in response to real-ambient PM exposure (Exp-NMN group) compared with the Exp-H2O group (all p<0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
Six-week-old male C57BL/6J mice were placed in a real-ambient PM exposure system or filtered air-equipped chambers (control) for 16 wk with or without NMN supplementation in drinking water (regarded as Con-H2O, Exp-H2O, Con-NMN and Exp-NMN groups, respectively) in Shijiazhuang City, China (n=20/group).
Summary · context
The effects of NMN supplementation (500mg/kg) on PM-induced chronic pulmonary inflammation were assessed, and its mechanism was characterized using single-cell transcriptomic sequencing (scRNA-seq) analysis of whole lung cells.
Show in the study text Report a problemFND-3745871203
04AnimalAnimal study (in vivo)Mechanism
In PM-exposed mice, the NMN group had fewer lung neutrophils compared with mice exposed without NMN.
In plain wordsIn PM-exposed mice, NMN was associated with fewer lung neutrophils compared with exposure without NMN.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseLung neutrophils
Study
mousen: not reported in source
male C57BL/6J mice exposed to ambient particulate matter
Comparator
PM-exposed mice without NMN
Outcome group
Immune response · Respiratory
Studied dose
500 mg/kg · drinking water · 16 weeksAs given in this study (mouse).
Source sentences
Summary
Mouse lung tissue isolated from the Exp-NMN group was characterized by fewer neutrophils, monocyte-derived cells, fibroblasts, and myeloid-derived suppressor cells induced by subchronic PM exposure as detected by scRNA-seq transcriptomic analysis.
2 context sentences(design, population, supporting numbers)
Summary · context
Six-week-old male C57BL/6J mice were placed in a real-ambient PM exposure system or filtered air-equipped chambers (control) for 16 wk with or without NMN supplementation in drinking water (regarded as Con-H2O, Exp-H2O, Con-NMN and Exp-NMN groups, respectively) in Shijiazhuang City, China (n=20/group).
Summary · context
The effects of NMN supplementation (500mg/kg) on PM-induced chronic pulmonary inflammation were assessed, and its mechanism was characterized using single-cell transcriptomic sequencing (scRNA-seq) analysis of whole lung cells.
Show in the study text Report a problemFND-3745871204
05AnimalAnimal study (in vivo)Mechanism
In PM-exposed mice, the NMN group had fewer lung monocyte-derived cells compared with mice exposed without NMN.
In plain wordsIn PM-exposed mice, NMN was associated with fewer lung monocyte-derived cells compared with exposure without NMN.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseLung monocyte-derived cells
Study
mousen: not reported in source
male C57BL/6J mice exposed to ambient particulate matter
Comparator
PM-exposed mice without NMN
Outcome group
Immune response · Respiratory
Studied dose
500 mg/kg · drinking water · 16 weeksAs given in this study (mouse).
Source sentences
Summary
Mouse lung tissue isolated from the Exp-NMN group was characterized by fewer neutrophils, monocyte-derived cells, fibroblasts, and myeloid-derived suppressor cells induced by subchronic PM exposure as detected by scRNA-seq transcriptomic analysis.
2 context sentences(design, population, supporting numbers)
Summary · context
Six-week-old male C57BL/6J mice were placed in a real-ambient PM exposure system or filtered air-equipped chambers (control) for 16 wk with or without NMN supplementation in drinking water (regarded as Con-H2O, Exp-H2O, Con-NMN and Exp-NMN groups, respectively) in Shijiazhuang City, China (n=20/group).
Summary · context
The effects of NMN supplementation (500mg/kg) on PM-induced chronic pulmonary inflammation were assessed, and its mechanism was characterized using single-cell transcriptomic sequencing (scRNA-seq) analysis of whole lung cells.
Show in the study text Report a problemFND-3745871205
06AnimalAnimal study (in vivo)Mechanism
In PM-exposed mice, the NMN group had fewer lung fibroblasts compared with mice exposed without NMN.
In plain wordsIn PM-exposed mice, NMN was associated with fewer lung fibroblasts compared with exposure without NMN.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseLung fibroblasts
Study
mousen: not reported in source
male C57BL/6J mice exposed to ambient particulate matter
Comparator
PM-exposed mice without NMN
Outcome group
Immune response · Respiratory
Studied dose
500 mg/kg · drinking water · 16 weeksAs given in this study (mouse).
Source sentences
Summary
Mouse lung tissue isolated from the Exp-NMN group was characterized by fewer neutrophils, monocyte-derived cells, fibroblasts, and myeloid-derived suppressor cells induced by subchronic PM exposure as detected by scRNA-seq transcriptomic analysis.
2 context sentences(design, population, supporting numbers)
Summary · context
Six-week-old male C57BL/6J mice were placed in a real-ambient PM exposure system or filtered air-equipped chambers (control) for 16 wk with or without NMN supplementation in drinking water (regarded as Con-H2O, Exp-H2O, Con-NMN and Exp-NMN groups, respectively) in Shijiazhuang City, China (n=20/group).
Summary · context
The effects of NMN supplementation (500mg/kg) on PM-induced chronic pulmonary inflammation were assessed, and its mechanism was characterized using single-cell transcriptomic sequencing (scRNA-seq) analysis of whole lung cells.
Show in the study text Report a problemFND-3745871206
07AnimalAnimal study (in vivo)Mechanism
In PM-exposed mice, the NMN group had fewer lung myeloid-derived suppressor cells compared with mice exposed without NMN.
In plain wordsIn PM-exposed mice, NMN was associated with fewer lung myeloid-derived suppressor cells compared with exposure without NMN.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseMyeloid-derived suppressor cells
Study
mousen: not reported in source
male C57BL/6J mice exposed to ambient particulate matter
Comparator
PM-exposed mice without NMN
Outcome group
Immune response · Respiratory
Studied dose
500 mg/kg · drinking water · 16 weeksAs given in this study (mouse).
Source sentences
Summary
Mouse lung tissue isolated from the Exp-NMN group was characterized by fewer neutrophils, monocyte-derived cells, fibroblasts, and myeloid-derived suppressor cells induced by subchronic PM exposure as detected by scRNA-seq transcriptomic analysis.
2 context sentences(design, population, supporting numbers)
Summary · context
Six-week-old male C57BL/6J mice were placed in a real-ambient PM exposure system or filtered air-equipped chambers (control) for 16 wk with or without NMN supplementation in drinking water (regarded as Con-H2O, Exp-H2O, Con-NMN and Exp-NMN groups, respectively) in Shijiazhuang City, China (n=20/group).
Summary · context
The effects of NMN supplementation (500mg/kg) on PM-induced chronic pulmonary inflammation were assessed, and its mechanism was characterized using single-cell transcriptomic sequencing (scRNA-seq) analysis of whole lung cells.
Show in the study text Report a problemFND-3745871207
08AnimalAnimal study (in vivo)Mechanism
In PM-exposed mice, NMN inhibited interleukin-17 signaling compared with exposure without NMN.
In plain wordsIn mice exposed to particulate matter, NMN reduced interleukin-17 signaling compared with exposure without NMN.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseInterleukin-17 signaling
Study
mousen: not reported in source
male C57BL/6J mice exposed to ambient particulate matter
Comparator
PM-exposed mice without NMN
Outcome group
Immune response · Respiratory
Studied dose
500 mg/kg · drinking water · 16 weeksAs given in this study (mouse).
Source sentences
Summary
The improved immune functions were further characterized by interleukin-17 signaling pathway inhibition and lower secretion of profibrotic cytokines in the Exp-NMN group compared with the Exp-H2O group.
2 context sentences(design, population, supporting numbers)
Summary · context
Six-week-old male C57BL/6J mice were placed in a real-ambient PM exposure system or filtered air-equipped chambers (control) for 16 wk with or without NMN supplementation in drinking water (regarded as Con-H2O, Exp-H2O, Con-NMN and Exp-NMN groups, respectively) in Shijiazhuang City, China (n=20/group).
Summary · context
The effects of NMN supplementation (500mg/kg) on PM-induced chronic pulmonary inflammation were assessed, and its mechanism was characterized using single-cell transcriptomic sequencing (scRNA-seq) analysis of whole lung cells.
Show in the study text Report a problemFND-3745871208
09AnimalAnimal study (in vivo)Mechanism
In PM-exposed mice, NMN reduced profibrotic cytokine secretion compared with exposure without NMN.
In plain wordsIn mice exposed to particulate matter, NMN reduced profibrotic cytokine secretion compared with exposure without NMN.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseProfibrotic cytokine secretion
Study
mousen: not reported in source
male C57BL/6J mice exposed to ambient particulate matter
Comparator
PM-exposed mice without NMN
Outcome group
Immune response · Respiratory
Studied dose
500 mg/kg · drinking water · 16 weeksAs given in this study (mouse).
Source sentences
Summary
The improved immune functions were further characterized by interleukin-17 signaling pathway inhibition and lower secretion of profibrotic cytokines in the Exp-NMN group compared with the Exp-H2O group.
2 context sentences(design, population, supporting numbers)
Summary · context
Six-week-old male C57BL/6J mice were placed in a real-ambient PM exposure system or filtered air-equipped chambers (control) for 16 wk with or without NMN supplementation in drinking water (regarded as Con-H2O, Exp-H2O, Con-NMN and Exp-NMN groups, respectively) in Shijiazhuang City, China (n=20/group).
Summary · context
The effects of NMN supplementation (500mg/kg) on PM-induced chronic pulmonary inflammation were assessed, and its mechanism was characterized using single-cell transcriptomic sequencing (scRNA-seq) analysis of whole lung cells.
Show in the study text Report a problemFND-3745871209
10AnimalAnimal study (in vivo)Mechanism
In PM-exposed mice, the NMN group had a lower proportion of differentiated myofibroblasts compared with mice exposed without NMN.
In plain wordsIn PM-exposed mice, NMN was associated with a lower proportion of differentiated myofibroblasts compared with exposure without NMN.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseDifferentiated myofibroblast proportion
Study
mousen: not reported in source
male C57BL/6J mice exposed to ambient particulate matter
Comparator
PM-exposed mice without NMN
Outcome group
Immune response · Respiratory
Studied dose
500 mg/kg · drinking water · 16 weeksAs given in this study (mouse).
Source sentences
Summary
In addition, reduced proportions of differentiated myofibroblasts and profibrotic interstitial macrophages were identified in the NMN-supplemented mice in response to PM exposure.
2 context sentences(design, population, supporting numbers)
Summary · context
Six-week-old male C57BL/6J mice were placed in a real-ambient PM exposure system or filtered air-equipped chambers (control) for 16 wk with or without NMN supplementation in drinking water (regarded as Con-H2O, Exp-H2O, Con-NMN and Exp-NMN groups, respectively) in Shijiazhuang City, China (n=20/group).
Summary · context
The effects of NMN supplementation (500mg/kg) on PM-induced chronic pulmonary inflammation were assessed, and its mechanism was characterized using single-cell transcriptomic sequencing (scRNA-seq) analysis of whole lung cells.
Show in the study text Report a problemFND-3745871210
11AnimalAnimal study (in vivo)Mechanism
In PM-exposed mice, the NMN group had a lower proportion of profibrotic interstitial macrophages compared with mice exposed without NMN.
In plain wordsIn PM-exposed mice, NMN was associated with a lower proportion of profibrotic interstitial macrophages compared with exposure without NMN.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseProfibrotic interstitial macrophage proportion
Study
mousen: not reported in source
male C57BL/6J mice exposed to ambient particulate matter
Comparator
PM-exposed mice without NMN
Outcome group
Immune response · Respiratory
Studied dose
500 mg/kg · drinking water · 16 weeksAs given in this study (mouse).
Source sentences
Summary
In addition, reduced proportions of differentiated myofibroblasts and profibrotic interstitial macrophages were identified in the NMN-supplemented mice in response to PM exposure.
2 context sentences(design, population, supporting numbers)
Summary · context
Six-week-old male C57BL/6J mice were placed in a real-ambient PM exposure system or filtered air-equipped chambers (control) for 16 wk with or without NMN supplementation in drinking water (regarded as Con-H2O, Exp-H2O, Con-NMN and Exp-NMN groups, respectively) in Shijiazhuang City, China (n=20/group).
Summary · context
The effects of NMN supplementation (500mg/kg) on PM-induced chronic pulmonary inflammation were assessed, and its mechanism was characterized using single-cell transcriptomic sequencing (scRNA-seq) analysis of whole lung cells.
Show in the study text Report a problemFND-3745871211
12AnimalAnimal study (in vivo)Mechanism
In PM-exposed mice, less immune suppression and altered pathological cell differentiation with NMN related to intracellular lipid metabolism activation.
In plain wordsIn mice, NMN-associated immune and cell changes related to activation of intracellular lipid metabolism.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseIntracellular lipid metabolism
Study
mousen: not reported in source
male C57BL/6J mice exposed to ambient particulate matter
Outcome group
Lipids · Respiratory
Studied dose
500 mg/kg · drinking water · 16 weeksAs given in this study (mouse).
Source sentences
Summary
Furthermore, less immune function suppression and altered differentiation of pathological cell phenotypes NMN was related to intracellular lipid metabolism activation.
2 context sentences(design, population, supporting numbers)
Summary · context
Six-week-old male C57BL/6J mice were placed in a real-ambient PM exposure system or filtered air-equipped chambers (control) for 16 wk with or without NMN supplementation in drinking water (regarded as Con-H2O, Exp-H2O, Con-NMN and Exp-NMN groups, respectively) in Shijiazhuang City, China (n=20/group).
Summary · context
The effects of NMN supplementation (500mg/kg) on PM-induced chronic pulmonary inflammation were assessed, and its mechanism was characterized using single-cell transcriptomic sequencing (scRNA-seq) analysis of whole lung cells.
Show in the study text Report a problemFND-3745871212

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

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