Lipidome Investigation of Carnosine Effect on Nude Mice Skin to Prevent UV-A Damage.

Zoanni B, Aiello G, Negre-Salvayre A, Aldini G, Carini M, D'Amato A

International journal of molecular sciences11 Jun 2023Cited by 7 (Europe PMC)

PMID37373157PMCIDPMC10297840DOI10.3390/ijms241210009PepBankPUB-37373157

Summary

The lipid profile of skin is fundamental in the maintenance of the protective barrier against the external environment. Signaling and constitutive lipids of this large organ are involved in inflammation, metabolism, aging, and wound healing, such as phospholipids, triglycerides, FFA, and sphingomyelin. Skin exposure to ultraviolet (UV) radiation results in a photoaging process that is an accelerated form of aging. UV-A radiation deeply penetrates the dermis and promotes damage to DNA, lipids, and proteins by increasing the generation of reactive oxygen species (ROS). Carnosine, an endogenous β-alanyl-L-histidine dipeptide, demonstrated antioxidant properties that prevent photoaging and modification of skin protein profiling, making carnosine a compelling ingredient to consider for use in dermatology. The aim of this research was to investigate the modification of skin lipidome after UV-A treatment in presence or not of topic administration of carnosine. Quantitative analyses based on high-resolution mass spectrometry of nude mice skin-extracted lipids resulted in several modifications of barrier composition after UV-A radiation, with or without carnosine treatment. In total, 328 out of 683 molecules showed significant alteration-262 after UV-A radiation and 126 after UV-A and carnosine treatment versus controls. Importantly, the increased oxidized TGs after UV-A radiation, responsible of dermis photoaging, were completely reverted by carnosine application to prevent the UV-A damage. Network analyses also showed that the production of ROS and the calcium and TNF signaling were modulated by UV-A and carnosine. In conclusion, lipidome analyses attested the carnosine activity to prevent the UV-A damage, reducing the lipid oxidation, the inflammation, and the dysregulation of lipid skin barrier.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study3

3 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Activity measurement
In UV-A-exposed nude mouse skin treated with carnosine, 126 lipid molecules were significantly altered versus controls.
In plain wordsIn mice, UV-A-exposed skin treated with carnosine showed significant alterations in 126 lipid molecules versus controls.
Peptide
L-carnosine
Outcome
MixedAltered skin lipid molecules
Study
mousen: not reported in source
UV-A-exposed nude mouse skin
Comparator
Controls
Outcome group
Skin and hair · Skin and wound
Studied dose
Dose not stated · topicalAs given in this study (mouse).
Source sentences
Summary
In total, 328 out of 683 molecules showed significant alteration-262 after UV-A radiation and 126 after UV-A and carnosine treatment versus controls.
2 context sentences(design, population, supporting numbers)
Summary · context
The aim of this research was to investigate the modification of skin lipidome after UV-A treatment in presence or not of topic administration of carnosine.
Summary · context
Quantitative analyses based on high-resolution mass spectrometry of nude mice skin-extracted lipids resulted in several modifications of barrier composition after UV-A radiation, with or without carnosine treatment.
Show in the study text Report a problemFND-3737315701
02AnimalAnimal study (in vivo)Efficacy outcome
In nude mouse skin, carnosine completely reversed the increase in oxidized triglycerides after UV-A radiation.
In plain wordsIn mice, carnosine completely reversed the increase in oxidized skin triglycerides after UV-A radiation.
Peptide
L-carnosine
Outcome
DecreaseOxidized triglycerides
Study
mousen: not reported in source
UV-A-exposed nude mouse skin
Comparator
UV-A radiation without carnosine
Outcome group
Skin and hair · Skin and wound
Studied dose
Dose not stated · topicalAs given in this study (mouse).
Source sentences
Summary
Importantly, the increased oxidized TGs after UV-A radiation, responsible of dermis photoaging, were completely reverted by carnosine application to prevent the UV-A damage.
2 context sentences(design, population, supporting numbers)
Summary · context
The aim of this research was to investigate the modification of skin lipidome after UV-A treatment in presence or not of topic administration of carnosine.
Summary · context
Quantitative analyses based on high-resolution mass spectrometry of nude mice skin-extracted lipids resulted in several modifications of barrier composition after UV-A radiation, with or without carnosine treatment.
Show in the study text Report a problemFND-3737315702
03AnimalAnimal study (in vivo)Mechanism
In nude mouse skin, network analyses showed modulation of ROS production, calcium signaling, and TNF signaling by UV-A and carnosine.
In plain wordsIn nude mouse skin, network analyses showed modulation of ROS production, calcium signaling, and TNF signaling by UV-A and carnosine.
Peptide
L-carnosine
Outcome
Direction not reportedNetwork modulation of ROS production, calcium signaling, and TNF signaling
Study
mousen: not reported in source
UV-A-exposed nude mouse skin
Outcome group
Receptor activity and mechanism · Skin and wound
Studied dose
Dose not stated · topicalAs given in this study (mouse).
Source sentences
Summary
Network analyses also showed that the production of ROS and the calcium and TNF signaling were modulated by UV-A and carnosine.
2 context sentences(design, population, supporting numbers)
Summary · context
The aim of this research was to investigate the modification of skin lipidome after UV-A treatment in presence or not of topic administration of carnosine.
Summary · context
Quantitative analyses based on high-resolution mass spectrometry of nude mice skin-extracted lipids resulted in several modifications of barrier composition after UV-A radiation, with or without carnosine treatment.
Show in the study text Report a problemFND-3737315703

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
27 Oct 2026, 18:20 UTC
Study results updated
Version 1
27 Oct 2026, 16:32 UTC
First added · source update from Europe PMC

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