Impact of quercetin, carnosine, and ozone in the cryopreservation on Nellore (Bos indicus) semen.

Dos Reis WVA, Pereira RR, Vieira M, da Cunha CCT, Acácio BR, Macedo GG, da Costa-E-Silva EV, Sampaio BFB

Animal reproduction31 Mar 2023Cited by 2 (Europe PMC)

PMID37034117PMCIDPMC10075187DOI10.1590/1984-3143-ar2022-0048PepBankPUB-37034117

Summary

The objective of this study was to reduce the effects of cryoinjury caused in bovine semen by cryopreservation. Ejaculates were collected from Nellore bulls and subjected to freezing in C (control), ozone (15, 30, and 60 µg mL^-1 of ozone), quercetin (25, 50, and 100 µg mL^-1 of quercetin), and carnosine groups (100, 200, and 300 ng mL^-1 of carnosine). Samples were evaluated post-thaw (M0) and post-rapid thermoresistance test (M30) for sperm kinetics (total motility, progressive motility, curvilinear speed, linearity and amplitude of lateral head displacement) and cell structure viability (plasma membrane integrity, acrosomal integrity, mitochondrial potential, membrane fluidity, and lipid peroxidation). There were no differences (P > 0.05) between the control, quercetin, and carnosine-treated groups for the parameters evaluated at M0 and M30. In turn, supplementation with ozone resulted in lower values for sperm kinetics (P ) and lower mitochondrial potential at M30 (P ). Quercetin and carnosine at the concentrations used did not promote significant gains in frozen semen, nor did they demonstrate cytotoxicity. We expected to obtain positive results with the use of ozone. Nonetheless, the addition was harmful to the parameters of sperm kinetics, and its effect was not observed as a possible pro-antioxidant. We believe that the fact that the gas did not harm the sperm structure opens avenues for tests with lower dosages, since, by reducing its concentration, we could minimize the damage to sperm kinetics.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study11

11 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueEfficacy outcome
In cryopreserved bull semen, carnosine did not significantly change total motility versus control at M0 and M30.
In plain wordsIn lab tests with bull semen, carnosine did not significantly change total motility versus control at M0 and M30.
Peptide
L-carnosine
Outcome
No significant changeTotal motility
Study
Bos indicusn: not reported in source
Cryopreserved Nellore bull semen
Comparator
Control
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
100, 200, and 300 ng mL-1As given in this study (Bos indicus).
Source sentences
Summary
There were no differences (P > 0.05) between the control, quercetin, and carnosine-treated groups for the parameters evaluated at M0 and M30.
3 context sentences(design, population, supporting numbers)
Summary · context
Samples were evaluated post-thaw (M0) and post-rapid thermoresistance test (M30) for sperm kinetics (total motility, progressive motility, curvilinear speed, linearity and amplitude of lateral head displacement)
Summary · context
Ejaculates were collected from Nellore bulls and subjected to freezing in C (control),
Summary · context
and carnosine groups (100, 200, and 300 ng mL^-1 of carnosine).
Show in the study text Report a problemFND-3703411701
02LabEx vivo tissueEfficacy outcome
In cryopreserved bull semen, carnosine did not significantly change progressive motility versus control at M0 and M30.
In plain wordsIn lab tests with bull semen, carnosine did not significantly change progressive motility versus control at M0 and M30.
Peptide
L-carnosine
Outcome
No significant changeProgressive motility
Study
Bos indicusn: not reported in source
Cryopreserved Nellore bull semen
Comparator
Control
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
100, 200, and 300 ng mL-1As given in this study (Bos indicus).
Source sentences
Summary
There were no differences (P > 0.05) between the control, quercetin, and carnosine-treated groups for the parameters evaluated at M0 and M30.
3 context sentences(design, population, supporting numbers)
Summary · context
Samples were evaluated post-thaw (M0) and post-rapid thermoresistance test (M30) for sperm kinetics (total motility, progressive motility, curvilinear speed, linearity and amplitude of lateral head displacement)
Summary · context
Ejaculates were collected from Nellore bulls and subjected to freezing in C (control),
Summary · context
and carnosine groups (100, 200, and 300 ng mL^-1 of carnosine).
Show in the study text Report a problemFND-3703411702
03LabEx vivo tissueEfficacy outcome
In cryopreserved bull semen, carnosine did not significantly change curvilinear speed versus control at M0 and M30.
In plain wordsIn lab tests with bull semen, carnosine did not significantly change curvilinear speed versus control at M0 and M30.
Peptide
L-carnosine
Outcome
No significant changeCurvilinear speed
Study
Bos indicusn: not reported in source
Cryopreserved Nellore bull semen
Comparator
Control
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
100, 200, and 300 ng mL-1As given in this study (Bos indicus).
Source sentences
Summary
There were no differences (P > 0.05) between the control, quercetin, and carnosine-treated groups for the parameters evaluated at M0 and M30.
3 context sentences(design, population, supporting numbers)
Summary · context
Samples were evaluated post-thaw (M0) and post-rapid thermoresistance test (M30) for sperm kinetics (total motility, progressive motility, curvilinear speed, linearity and amplitude of lateral head displacement)
Summary · context
Ejaculates were collected from Nellore bulls and subjected to freezing in C (control),
Summary · context
and carnosine groups (100, 200, and 300 ng mL^-1 of carnosine).
Show in the study text Report a problemFND-3703411703
04LabEx vivo tissueEfficacy outcome
In cryopreserved bull semen, carnosine did not significantly change linearity versus control at M0 and M30.
In plain wordsIn lab tests with bull semen, carnosine did not significantly change linearity versus control at M0 and M30.
Peptide
L-carnosine
Outcome
No significant changeLinearity
Study
Bos indicusn: not reported in source
Cryopreserved Nellore bull semen
Comparator
Control
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
100, 200, and 300 ng mL-1As given in this study (Bos indicus).
Source sentences
Summary
There were no differences (P > 0.05) between the control, quercetin, and carnosine-treated groups for the parameters evaluated at M0 and M30.
3 context sentences(design, population, supporting numbers)
Summary · context
Samples were evaluated post-thaw (M0) and post-rapid thermoresistance test (M30) for sperm kinetics (total motility, progressive motility, curvilinear speed, linearity and amplitude of lateral head displacement)
Summary · context
Ejaculates were collected from Nellore bulls and subjected to freezing in C (control),
Summary · context
and carnosine groups (100, 200, and 300 ng mL^-1 of carnosine).
Show in the study text Report a problemFND-3703411704
05LabEx vivo tissueEfficacy outcome
In cryopreserved bull semen, carnosine did not significantly change lateral head displacement amplitude versus control at M0 and M30.
In plain wordsIn lab tests with bull semen, carnosine did not significantly change lateral head displacement amplitude versus control at M0 and M30.
Peptide
L-carnosine
Outcome
No significant changeAmplitude of lateral head displacement
Study
Bos indicusn: not reported in source
Cryopreserved Nellore bull semen
Comparator
Control
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
100, 200, and 300 ng mL-1As given in this study (Bos indicus).
Source sentences
Summary
There were no differences (P > 0.05) between the control, quercetin, and carnosine-treated groups for the parameters evaluated at M0 and M30.
3 context sentences(design, population, supporting numbers)
Summary · context
Samples were evaluated post-thaw (M0) and post-rapid thermoresistance test (M30) for sperm kinetics (total motility, progressive motility, curvilinear speed, linearity and amplitude of lateral head displacement)
Summary · context
Ejaculates were collected from Nellore bulls and subjected to freezing in C (control),
Summary · context
and carnosine groups (100, 200, and 300 ng mL^-1 of carnosine).
Show in the study text Report a problemFND-3703411705
06LabEx vivo tissueEfficacy outcome
In cryopreserved bull semen, carnosine did not significantly change plasma membrane integrity versus control at M0 and M30.
In plain wordsIn lab tests with bull semen, carnosine did not significantly change plasma membrane integrity versus control at M0 and M30.
Peptide
L-carnosine
Outcome
No significant changePlasma membrane integrity
Study
Bos indicusn: not reported in source
Cryopreserved Nellore bull semen
Comparator
Control
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
100, 200, and 300 ng mL-1As given in this study (Bos indicus).
Source sentences
Summary
There were no differences (P > 0.05) between the control, quercetin, and carnosine-treated groups for the parameters evaluated at M0 and M30.
3 context sentences(design, population, supporting numbers)
Summary · context
and cell structure viability (plasma membrane integrity, acrosomal integrity, mitochondrial potential, membrane fluidity, and lipid peroxidation).
Summary · context
Ejaculates were collected from Nellore bulls and subjected to freezing in C (control),
Summary · context
and carnosine groups (100, 200, and 300 ng mL^-1 of carnosine).
Show in the study text Report a problemFND-3703411706
07LabEx vivo tissueEfficacy outcome
In cryopreserved bull semen, carnosine did not significantly change acrosomal integrity versus control at M0 and M30.
In plain wordsIn lab tests with bull semen, carnosine did not significantly change acrosomal integrity versus control at M0 and M30.
Peptide
L-carnosine
Outcome
No significant changeAcrosomal integrity
Study
Bos indicusn: not reported in source
Cryopreserved Nellore bull semen
Comparator
Control
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
100, 200, and 300 ng mL-1As given in this study (Bos indicus).
Source sentences
Summary
There were no differences (P > 0.05) between the control, quercetin, and carnosine-treated groups for the parameters evaluated at M0 and M30.
3 context sentences(design, population, supporting numbers)
Summary · context
and cell structure viability (plasma membrane integrity, acrosomal integrity, mitochondrial potential, membrane fluidity, and lipid peroxidation).
Summary · context
Ejaculates were collected from Nellore bulls and subjected to freezing in C (control),
Summary · context
and carnosine groups (100, 200, and 300 ng mL^-1 of carnosine).
Show in the study text Report a problemFND-3703411707
08LabEx vivo tissueEfficacy outcome
In cryopreserved bull semen, carnosine did not significantly change mitochondrial potential versus control at M0 and M30.
In plain wordsIn lab tests with bull semen, carnosine did not significantly change mitochondrial potential versus control at M0 and M30.
Peptide
L-carnosine
Outcome
No significant changeMitochondrial potential
Study
Bos indicusn: not reported in source
Cryopreserved Nellore bull semen
Comparator
Control
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
100, 200, and 300 ng mL-1As given in this study (Bos indicus).
Source sentences
Summary
There were no differences (P > 0.05) between the control, quercetin, and carnosine-treated groups for the parameters evaluated at M0 and M30.
3 context sentences(design, population, supporting numbers)
Summary · context
and cell structure viability (plasma membrane integrity, acrosomal integrity, mitochondrial potential, membrane fluidity, and lipid peroxidation).
Summary · context
Ejaculates were collected from Nellore bulls and subjected to freezing in C (control),
Summary · context
and carnosine groups (100, 200, and 300 ng mL^-1 of carnosine).
Show in the study text Report a problemFND-3703411708
09LabEx vivo tissueEfficacy outcome
In cryopreserved bull semen, carnosine did not significantly change membrane fluidity versus control at M0 and M30.
In plain wordsIn lab tests with bull semen, carnosine did not significantly change membrane fluidity versus control at M0 and M30.
Peptide
L-carnosine
Outcome
No significant changeMembrane fluidity
Study
Bos indicusn: not reported in source
Cryopreserved Nellore bull semen
Comparator
Control
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
100, 200, and 300 ng mL-1As given in this study (Bos indicus).
Source sentences
Summary
There were no differences (P > 0.05) between the control, quercetin, and carnosine-treated groups for the parameters evaluated at M0 and M30.
3 context sentences(design, population, supporting numbers)
Summary · context
and cell structure viability (plasma membrane integrity, acrosomal integrity, mitochondrial potential, membrane fluidity, and lipid peroxidation).
Summary · context
Ejaculates were collected from Nellore bulls and subjected to freezing in C (control),
Summary · context
and carnosine groups (100, 200, and 300 ng mL^-1 of carnosine).
Show in the study text Report a problemFND-3703411709
10LabEx vivo tissueEfficacy outcome
In cryopreserved bull semen, carnosine did not significantly change lipid peroxidation versus control at M0 and M30.
In plain wordsIn lab tests with bull semen, carnosine did not significantly change lipid peroxidation versus control at M0 and M30.
Peptide
L-carnosine
Outcome
No significant changeLipid peroxidation
Study
Bos indicusn: not reported in source
Cryopreserved Nellore bull semen
Comparator
Control
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
100, 200, and 300 ng mL-1As given in this study (Bos indicus).
Source sentences
Summary
There were no differences (P > 0.05) between the control, quercetin, and carnosine-treated groups for the parameters evaluated at M0 and M30.
3 context sentences(design, population, supporting numbers)
Summary · context
and cell structure viability (plasma membrane integrity, acrosomal integrity, mitochondrial potential, membrane fluidity, and lipid peroxidation).
Summary · context
Ejaculates were collected from Nellore bulls and subjected to freezing in C (control),
Summary · context
and carnosine groups (100, 200, and 300 ng mL^-1 of carnosine).
Show in the study text Report a problemFND-3703411710
11LabEx vivo tissueSafety outcome
In cryopreserved bull semen, carnosine did not demonstrate cytotoxicity at the tested concentrations.
In plain wordsIn lab tests with bull semen, carnosine did not demonstrate cell toxicity at the tested concentrations.
Peptide
L-carnosine
Outcome
No significant changeCytotoxicity
Study
Bos indicusn: not reported in source
Cryopreserved Nellore bull semen
Comparator
Control
Outcome group
Adverse events · Reproductive and sexual health
Studied dose
100, 200, and 300 ng mL-1As given in this study (Bos indicus).
Source sentences
Summary
Quercetin and carnosine at the concentrations used did not promote significant gains in frozen semen, nor did they demonstrate cytotoxicity.
2 context sentences(design, population, supporting numbers)
Summary · context
Ejaculates were collected from Nellore bulls and subjected to freezing in C (control),
Summary · context
and carnosine groups (100, 200, and 300 ng mL^-1 of carnosine).
Show in the study text Report a problemFND-3703411711

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
27 Oct 2026, 18:20 UTC
Study results updated
Version 1
27 Oct 2026, 16:32 UTC
First added · source update from Europe PMC

Subjects

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Keywords

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