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Study · In vitro (cells) · mouse · 2023

8 results

Kisspeptin induces Kiss-1 and GnRH gene expression in mHypoA-55 hypothalamic cell models: Involvement of the ERK and PKA signaling pathways.

Tumurbaatar T, Kanasaki H, Yacca SS, Cairang Z, Tumurgan Z, Oride A, Okada H, Kyo S

General and comparative endocrinology16 Mar 2023Cited by 3 (Europe PMC)

PMID36933747 (opens in a new tab)DOI10.1016/j.ygcen.2023.114260 (opens in a new tab)PepBankPUB-36933747
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

8 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In Kiss-1R-overexpressing mHypoA-55 cells, KP10 increased Kiss-1 gene expression.

    In plain wordsIn mouse brain cells grown in a dish, kisspeptin‑10 raised the activity of the Kiss-1 gene.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseKiss-1 gene expression
    Study

    mousen: not reported in source

    Kiss-1R-overexpressing mHypoA-55 hypothalamic cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated · in vitroAs given in this study (mouse).

    Source sentences

    Summary
    Here, we found that kisspeptin 10 (KP10) increased Kiss-1 (encoding kisspeptin) and GnRH gene expression in kisspeptin receptor (Kiss-1R)-overexpressing mHypoA-55 cells.
  2. 02LabIn vitro (cells)Mechanism

    In Kiss-1R-overexpressing mHypoA-55 cells, KP10 increased GnRH gene expression.

    In plain wordsIn mouse brain cells grown in a dish, kisspeptin‑10 raised the activity of the GnRH gene.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseGnRH gene expression
    Study

    mousen: not reported in source

    Kiss-1R-overexpressing mHypoA-55 hypothalamic cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated · in vitroAs given in this study (mouse).

    Source sentences

    Summary
    Here, we found that kisspeptin 10 (KP10) increased Kiss-1 (encoding kisspeptin) and GnRH gene expression in kisspeptin receptor (Kiss-1R)-overexpressing mHypoA-55 cells.
  3. 03LabIn vitro (cells)Mechanism

    In mHypoA-55 cells, KP10 greatly increased serum response element (SRE) promoter activity, a target of ERK (20.0-fold).

    In plain wordsIn mouse brain cells grown in a dish, kisspeptin‑10 strongly switched on an ERK pathway signal.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseSRE promoter activity
    Study

    mousen: not reported in source

    Kiss-1R-overexpressing mHypoA-55 hypothalamic cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated · in vitroAs given in this study (mouse).

    Measurements

    SRE promoter activity
    20 fold
    KP10, mHypoA-55 cells

    Source sentences

    Summary
    KP10 greatly increased serum response element (SRE) promoter activity, which is a target of extracellular signal-regulated kinase (ERK) (20.0 ± 2.54-fold).
    1 context sentence
    Summary · context
    Here, we found that kisspeptin 10 (KP10) increased Kiss-1 (encoding kisspeptin) and GnRH gene expression in kisspeptin receptor (Kiss-1R)-overexpressing mHypoA-55 cells.
  4. 04LabIn vitro (cells)Mechanism

    In mHypoA-55 cells, KP10 increased cAMP-response element (CRE) promoter activity (2.32-fold).

    In plain wordsIn mouse brain cells grown in a dish, kisspeptin‑10 switched on a cAMP pathway signal.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseCRE promoter activity
    Study

    mousen: not reported in source

    Kiss-1R-overexpressing mHypoA-55 hypothalamic cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated · in vitroAs given in this study (mouse).

    Measurements

    CRE promoter activity
    2.32 fold
    KP10, mHypoA-55 cells

    Source sentences

    Summary
    KP10 also increased cAMP-response element (CRE) promoter activity in these cells (2.32 ± 0.36-fold).
    1 context sentence
    Summary · context
    Here, we found that kisspeptin 10 (KP10) increased Kiss-1 (encoding kisspeptin) and GnRH gene expression in kisspeptin receptor (Kiss-1R)-overexpressing mHypoA-55 cells.
  5. 05LabIn vitro (cells)Mechanism

    In mHypoA-55 cells, the MEKK inhibitor PD098095 prevented the KP10-increased SRE promoter activity, and PD098059 inhibited the KP10-induced CRE promoter activity.

    In plain wordsIn mouse brain cells grown in a dish, blocking MEK kinase stopped kisspeptin‑10 from switching on two pathway signals.

    Peptide
    Kisspeptin-10
    Outcome
    Not applicableDependence of KP10 SRE and CRE promoter activity on MEK kinase
    Study

    mousen: not reported in source

    Kiss-1R-overexpressing mHypoA-55 hypothalamic cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated · in vitroAs given in this study (mouse).

    Source sentences

    Summary
    KP10-increased SRE promoter activity was significantly prevented in the presence of PD098095, a MEK kinase (MEKK) inhibitor, and KP10-induced CRE promoter activity was also inhibited by PD098059.
    1 context sentence
    Summary · context
    Here, we found that kisspeptin 10 (KP10) increased Kiss-1 (encoding kisspeptin) and GnRH gene expression in kisspeptin receptor (Kiss-1R)-overexpressing mHypoA-55 cells.
  6. 06LabIn vitro (cells)Mechanism

    In mHypoA-55 cells, the PKA inhibitor H89 significantly inhibited the KP10 induction of SRE and CRE promoters.

    In plain wordsIn mouse brain cells grown in a dish, blocking PKA stopped kisspeptin‑10 from switching on two pathway signals.

    Peptide
    Kisspeptin-10
    Outcome
    Not applicableDependence of KP10 SRE and CRE promoter activity on PKA
    Study

    mousen: not reported in source

    Kiss-1R-overexpressing mHypoA-55 hypothalamic cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated · in vitroAs given in this study (mouse).

    Source sentences

    Summary
    Similarly, H89, a protein kinase A (PKA) inhibitor, significantly inhibited the KP10 induction of SRE and CRE promoters.
    1 context sentence
    Summary · context
    Here, we found that kisspeptin 10 (KP10) increased Kiss-1 (encoding kisspeptin) and GnRH gene expression in kisspeptin receptor (Kiss-1R)-overexpressing mHypoA-55 cells.
  7. 07LabIn vitro (cells)Mechanism

    In mHypoA-55 cells, the MEK inhibitor PD098059 inhibited KP10-induced Kiss-1 and GnRH gene expression.

    In plain wordsIn mouse brain cells grown in a dish, blocking MEK stopped kisspeptin‑10 from raising the Kiss-1 and GnRH genes.

    Peptide
    Kisspeptin-10
    Outcome
    Not applicableDependence of KP10-induced Kiss-1 and GnRH expression on MEK kinase
    Study

    mousen: not reported in source

    Kiss-1R-overexpressing mHypoA-55 hypothalamic cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated · in vitroAs given in this study (mouse).

    Source sentences

    Summary
    KP10-induced Kiss-1 and GnRH gene expressions were inhibited in the presence of PD098059.
    1 context sentence
    Summary · context
    Here, we found that kisspeptin 10 (KP10) increased Kiss-1 (encoding kisspeptin) and GnRH gene expression in kisspeptin receptor (Kiss-1R)-overexpressing mHypoA-55 cells.
  8. 08LabIn vitro (cells)Mechanism

    In mHypoA-55 cells, the PKA inhibitor H89 significantly inhibited the KP10-induced increase in Kiss-1 and GnRH.

    In plain wordsIn mouse brain cells grown in a dish, blocking PKA stopped kisspeptin‑10 from raising the Kiss-1 and GnRH genes.

    Peptide
    Kisspeptin-10
    Outcome
    Not applicableDependence of KP10-induced Kiss-1 and GnRH expression on PKA
    Study

    mousen: not reported in source

    Kiss-1R-overexpressing mHypoA-55 hypothalamic cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated · in vitroAs given in this study (mouse).

    Source sentences

    Summary
    Likewise, H89 significantly inhibited the KP10-induced increase in Kiss-1 and GnRH.
    1 context sentence
    Summary · context
    Here, we found that kisspeptin 10 (KP10) increased Kiss-1 (encoding kisspeptin) and GnRH gene expression in kisspeptin receptor (Kiss-1R)-overexpressing mHypoA-55 cells.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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