Damage-induced NAD release activates intestinal CD4+ and CD8+ T cell via P2X7R signaling.

Bu F, Huang S, Yang X, Wei L, Zhang D, Zhang Z, Tian D

Cellular immunology2 Feb 2023

PMID36746070DOI10.1016/j.cellimm.2023.104677PepBankPUB-36746070

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study15

13 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In mice, NAD injection directly impaired gastrointestinal motility.
In plain wordsIn mice, NAD injection impaired movement through the gastrointestinal tract.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
DecreaseGastrointestinal motility
Study
mousen: not reported in source
Intestinal manipulation postoperative ileus mice
Outcome group
Gastrointestinal protection · Gastrointestinal
Studied dose
Dose not stated
Source sentences
Summary
The concentration of extracellular NAD significantly increased after IM administration, and NAD directly impaired gastrointestinal motility.
3 context sentences(design, population, supporting numbers)
Summary · context
POI in mice was induced via intestinal manipulation (IM).
Summary · context
The concentration of nicotinamide adenine dinucleotide (NAD) was detected after IM.
Summary · context
The gastrointestinal motility of the mice was assessed after IM or NAD injection.
Report a problemFND-3674607002
02AnimalAnimal study (in vivo)Mechanism
In mice, NAD injection increased TNF-α expression in intestinal CD8+ T cells.
In plain wordsIn mice, NAD injection increased TNF-α expression in intestinal CD8+ T cells.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
IncreaseCD8 T cell TNF-alpha expression
Study
mousen: not reported in source
Intestinal manipulation postoperative ileus mice
Outcome group
Immune response · Immune and inflammation
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
Intraperitoneal injection of NAD promoted the expression of TNF-α in intestinal CD8+ and CD4+ T cells, but only IFN-γ production by CD8+ T cells was significantly promoted by NAD injection.
3 context sentences(design, population, supporting numbers)
Summary · context
POI in mice was induced via intestinal manipulation (IM).
Summary · context
The concentration of nicotinamide adenine dinucleotide (NAD) was detected after IM.
Summary · context
The gastrointestinal motility of the mice was assessed after IM or NAD injection.
Report a problemFND-3674607003
03AnimalAnimal study (in vivo)Mechanism
In mice, NAD injection increased TNF-α expression in intestinal CD4+ T cells.
In plain wordsIn mice, NAD injection increased TNF-α expression in intestinal CD4+ T cells.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
IncreaseCD4 T cell TNF-alpha expression
Study
mousen: not reported in source
Intestinal manipulation postoperative ileus mice
Outcome group
Immune response · Immune and inflammation
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
Intraperitoneal injection of NAD promoted the expression of TNF-α in intestinal CD8+ and CD4+ T cells, but only IFN-γ production by CD8+ T cells was significantly promoted by NAD injection.
3 context sentences(design, population, supporting numbers)
Summary · context
POI in mice was induced via intestinal manipulation (IM).
Summary · context
The concentration of nicotinamide adenine dinucleotide (NAD) was detected after IM.
Summary · context
The gastrointestinal motility of the mice was assessed after IM or NAD injection.
Report a problemFND-3674607004
04AnimalAnimal study (in vivo)Mechanism
In mice, NAD injection significantly increased IFN-γ production by intestinal CD8+ T cells.
In plain wordsIn mice, NAD injection increased IFN-γ production by intestinal CD8+ T cells.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
IncreaseCD8 T cell IFN-gamma production
Study
mousen: not reported in source
Intestinal manipulation postoperative ileus mice
Outcome group
Immune response · Immune and inflammation
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
Intraperitoneal injection of NAD promoted the expression of TNF-α in intestinal CD8+ and CD4+ T cells, but only IFN-γ production by CD8+ T cells was significantly promoted by NAD injection.
3 context sentences(design, population, supporting numbers)
Summary · context
POI in mice was induced via intestinal manipulation (IM).
Summary · context
The concentration of nicotinamide adenine dinucleotide (NAD) was detected after IM.
Summary · context
The gastrointestinal motility of the mice was assessed after IM or NAD injection.
Report a problemFND-3674607005
05AnimalAnimal study (in vivo)Mechanism
In mice, NAD injection did not significantly promote IFN-γ production by intestinal CD4+ T cells.
In plain wordsIn mice, NAD injection did not significantly increase IFN-γ production by intestinal CD4+ T cells.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
No significant changeCD4 T cell IFN-gamma production
Study
mousen: not reported in source
Intestinal manipulation postoperative ileus mice
Outcome group
Immune response · Immune and inflammation
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
Intraperitoneal injection of NAD promoted the expression of TNF-α in intestinal CD8+ and CD4+ T cells, but only IFN-γ production by CD8+ T cells was significantly promoted by NAD injection.
3 context sentences(design, population, supporting numbers)
Summary · context
POI in mice was induced via intestinal manipulation (IM).
Summary · context
The concentration of nicotinamide adenine dinucleotide (NAD) was detected after IM.
Summary · context
The gastrointestinal motility of the mice was assessed after IM or NAD injection.
Report a problemFND-3674607006
06AnimalAnimal study (in vivo)Mechanism
In mice, NAD administration decreased granzyme B production in intestinal CD8+ T cells.
In plain wordsIn mice, NAD administration reduced granzyme B production in intestinal CD8+ T cells.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
DecreaseCD8 T cell granzyme B production
Study
mousen: not reported in source
Intestinal manipulation postoperative ileus mice
Outcome group
Immune response · Immune and inflammation
Studied dose
Dose not stated
Source sentences
Summary
Granzyme B production in CD8+ and CD4+ T cells decreased after administration.
3 context sentences(design, population, supporting numbers)
Summary · context
POI in mice was induced via intestinal manipulation (IM).
Summary · context
The concentration of nicotinamide adenine dinucleotide (NAD) was detected after IM.
Summary · context
The gastrointestinal motility of the mice was assessed after IM or NAD injection.
Report a problemFND-3674607007
07AnimalAnimal study (in vivo)Mechanism
In mice, NAD administration decreased granzyme B production in intestinal CD4+ T cells.
In plain wordsIn mice, NAD administration reduced granzyme B production in intestinal CD4+ T cells.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
DecreaseCD4 T cell granzyme B production
Study
mousen: not reported in source
Intestinal manipulation postoperative ileus mice
Outcome group
Immune response · Immune and inflammation
Studied dose
Dose not stated
Source sentences
Summary
Granzyme B production in CD8+ and CD4+ T cells decreased after administration.
3 context sentences(design, population, supporting numbers)
Summary · context
POI in mice was induced via intestinal manipulation (IM).
Summary · context
The concentration of nicotinamide adenine dinucleotide (NAD) was detected after IM.
Summary · context
The gastrointestinal motility of the mice was assessed after IM or NAD injection.
Report a problemFND-3674607008
08LabIn vitro (cells)Mechanism
In intestinal T cells in vitro, NAD stimulation reproduced increased TNF-α expression in the CD8+ subset.
In plain wordsIn cells, NAD stimulation increased TNF-α expression in intestinal CD8+ T cells.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
IncreaseCD8 T cell TNF-alpha expression in vitro
Study
mousen: not reported in source
Intestinal CD3+ T cells
Outcome group
Immune response · Immune and inflammation
Studied dose
Dose not stated
Source sentences
Summary
Concordantly, the same results were observed in NAD stimulation of intestinal CD3+ T cells in vitro.
2 context sentences(design, population, supporting numbers)
Summary · context
Intraperitoneal injection of NAD promoted the expression of TNF-α in intestinal CD8+ and CD4+ T cells, but only IFN-γ production by CD8+ T cells was significantly promoted by NAD injection.
Summary · context
Cytokine production and calcium influx in T cells were investigated after NAD stimulation using flow cytometry.
Report a problemFND-3674607009
09LabIn vitro (cells)Mechanism
In intestinal T cells in vitro, NAD stimulation reproduced increased TNF-α expression in the CD4+ subset.
In plain wordsIn cells, NAD stimulation increased TNF-α expression in intestinal CD4+ T cells.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
IncreaseCD4 T cell TNF-alpha expression in vitro
Study
mousen: not reported in source
Intestinal CD3+ T cells
Outcome group
Immune response · Immune and inflammation
Studied dose
Dose not stated
Source sentences
Summary
Concordantly, the same results were observed in NAD stimulation of intestinal CD3+ T cells in vitro.
2 context sentences(design, population, supporting numbers)
Summary · context
Intraperitoneal injection of NAD promoted the expression of TNF-α in intestinal CD8+ and CD4+ T cells, but only IFN-γ production by CD8+ T cells was significantly promoted by NAD injection.
Summary · context
Cytokine production and calcium influx in T cells were investigated after NAD stimulation using flow cytometry.
Report a problemFND-3674607010
10LabIn vitro (cells)Mechanism
In intestinal T cells in vitro, NAD stimulation reproduced increased IFN-γ production in the CD8+ subset.
In plain wordsIn cells, NAD stimulation increased IFN-γ production in intestinal CD8+ T cells.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
IncreaseCD8 T cell IFN-gamma production in vitro
Study
mousen: not reported in source
Intestinal CD3+ T cells
Outcome group
Immune response · Immune and inflammation
Studied dose
Dose not stated
Source sentences
Summary
Concordantly, the same results were observed in NAD stimulation of intestinal CD3+ T cells in vitro.
2 context sentences(design, population, supporting numbers)
Summary · context
Intraperitoneal injection of NAD promoted the expression of TNF-α in intestinal CD8+ and CD4+ T cells, but only IFN-γ production by CD8+ T cells was significantly promoted by NAD injection.
Summary · context
Cytokine production and calcium influx in T cells were investigated after NAD stimulation using flow cytometry.
Report a problemFND-3674607011
11LabIn vitro (cells)Mechanism
In intestinal T cells in vitro, NAD stimulation reproduced the lack of significant IFN-γ promotion in the CD4+ subset.
In plain wordsIn cells, NAD stimulation did not significantly increase IFN-γ production in intestinal CD4+ T cells.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
No significant changeCD4 T cell IFN-gamma production in vitro
Study
mousen: not reported in source
Intestinal CD3+ T cells
Outcome group
Immune response · Immune and inflammation
Studied dose
Dose not stated
Source sentences
Summary
Concordantly, the same results were observed in NAD stimulation of intestinal CD3+ T cells in vitro.
2 context sentences(design, population, supporting numbers)
Summary · context
Intraperitoneal injection of NAD promoted the expression of TNF-α in intestinal CD8+ and CD4+ T cells, but only IFN-γ production by CD8+ T cells was significantly promoted by NAD injection.
Summary · context
Cytokine production and calcium influx in T cells were investigated after NAD stimulation using flow cytometry.
Report a problemFND-3674607012
12LabIn vitro (cells)Mechanism
In intestinal T cells in vitro, NAD stimulation reproduced decreased granzyme B production in the CD8+ subset.
In plain wordsIn cells, NAD stimulation reduced granzyme B production in intestinal CD8+ T cells.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
DecreaseCD8 T cell granzyme B production in vitro
Study
mousen: not reported in source
Intestinal CD3+ T cells
Outcome group
Immune response · Immune and inflammation
Studied dose
Dose not stated
Source sentences
Summary
Concordantly, the same results were observed in NAD stimulation of intestinal CD3+ T cells in vitro.
2 context sentences(design, population, supporting numbers)
Summary · context
Granzyme B production in CD8+ and CD4+ T cells decreased after administration.
Summary · context
Cytokine production and calcium influx in T cells were investigated after NAD stimulation using flow cytometry.
Report a problemFND-3674607013
13LabIn vitro (cells)Mechanism
In intestinal T cells in vitro, NAD stimulation reproduced decreased granzyme B production in the CD4+ subset.
In plain wordsIn cells, NAD stimulation reduced granzyme B production in intestinal CD4+ T cells.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
DecreaseCD4 T cell granzyme B production in vitro
Study
mousen: not reported in source
Intestinal CD3+ T cells
Outcome group
Immune response · Immune and inflammation
Studied dose
Dose not stated
Source sentences
Summary
Concordantly, the same results were observed in NAD stimulation of intestinal CD3+ T cells in vitro.
2 context sentences(design, population, supporting numbers)
Summary · context
Granzyme B production in CD8+ and CD4+ T cells decreased after administration.
Summary · context
Cytokine production and calcium influx in T cells were investigated after NAD stimulation using flow cytometry.
Report a problemFND-3674607014

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

14AnimalEndogenous levelAnimal study (in vivo)Mechanism
In mice, intestinal manipulation significantly increased natural extracellular NAD concentration.
In plain wordsIn mice, intestinal manipulation increased the body's own extracellular NAD concentration.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
IncreaseExtracellular NAD concentration
Study
mousen: not reported in source
Intestinal manipulation postoperative ileus mice
Outcome group
Inflammation markers · Gastrointestinal
Source sentences
Summary
The concentration of extracellular NAD significantly increased after IM administration, and NAD directly impaired gastrointestinal motility.
3 context sentences(design, population, supporting numbers)
Summary · context
POI in mice was induced via intestinal manipulation (IM).
Summary · context
The concentration of nicotinamide adenine dinucleotide (NAD) was detected after IM.
Summary · context
The gastrointestinal motility of the mice was assessed after IM or NAD injection.
This result measures the body's own NAD+ (nicotinamide adenine dinucleotide). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3674607001

Studies that did not test the peptide alone1

In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking NAD+ (nicotinamide adenine dinucleotide).

15LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cells)Mechanism
In intestinal T cell experiments, ART2.2 blockade significantly diminished the pro-inflammatory effect of NAD.
In plain wordsIn cells, ART2.2 blockade reduced the inflammatory effect of NAD.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
DecreaseInflammatory response after ART2.2 blockade
Study
mousen: not reported in source
NAD-stimulated intestinal T cells
Outcome group
Immune response · Immune and inflammation
Source sentences
Summary
Blocking the P2X7R-related membrane enzyme ART2.2 significantly diminished the pro-inflammatory effect of NAD.
1 context sentence(design, population, supporting numbers)
Summary · context
Cytokine production and calcium influx in T cells were investigated after NAD stimulation using flow cytometry.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-3674607015

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
14 Oct 2026, 05:02 UTC
Study results updated
Version 1
14 Oct 2026, 01:46 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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MeSH terms

Keywords

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