Does kisspeptin exert a local modulatory effect on bovine ovarian steroidogenesis?

Mattar D, Cheewasopit W, Samir M, Knight PG

Reproduction & fertility23 Feb 2023Cited by 3 (Europe PMC)

PMID36745024PMCIDPMC9986390DOI10.1530/raf-22-0088PepBankPUB-36745024

Summary

Kisspeptin, a hypothalamic neuropeptide encoded by the KISS1 gene, has a pivotal role in promoting GnRH secretion in mammals. Kisspeptin and its receptor (KISS1R) are also expressed in certain peripheral tissues including gonads, suggesting intra-gonadal roles. Such actions at the level of the bovine ovary have not been explored previously. The current aims were to determine whether KISS1 and its receptor (KISS1R) are expressed in the bovine ovary and whether kisspeptin or a kisspeptin antagonist can modulate ovarian steroid production by cultured ovarian cells. Granulosa (GC) and theca interna (TC) were collected from antral follicles (3-18 mm) categorized into five class sizes. Early, mid and regressing corpora lutea (CL) were also collected for RT-qPCR analysis of KISS1 and KISS1R expression. Bovine TC and GC cultured under both non-luteinizing (serum-free) and luteinizing (serum-supplemented) conditions were treated for 4 days with kisspeptin-10 (10-10-10-6M) or kisspeptin antagonist (p234; 10-10-10-6M), alone and in combination with either FSH (GC), LH (TC) or forskolin (luteinized GC/TC). Steroid secretion (GC: oestradiol, progesterone; TC: androstenedione, progesterone; luteinized GC/TC: progesterone) was measured by ELISA and viable cell number determined by neutral red uptake assay. KISS1 and KISS1R transcripts were detected in TC, GC and CL with significant differences between follicle categories and CL stages. However, neither kisspeptin-10 nor kisspeptin antagonist affected steroid secretion or viable cell number in any of the four ovarian cell culture models. As such, the hypothesis that kisspeptin has a direct intra-ovarian role to modulate follicular or luteal steroidogenesis, or cell proliferation/survival, is not supported.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study3

2 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In four bovine ovarian cell culture models, kisspeptin‑10 did not affect steroid secretion.
In plain wordsIn cow ovary cells grown in a dish, kisspeptin‑10 did not change steroid hormone release.
Peptide
Kisspeptin-10
Outcome
No significant changeSteroid secretion by cultured ovarian cells
Study
bovinen: not reported in source
cultured bovine granulosa and theca interna cells, non-luteinizing and luteinizing conditions
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
10-10-10-6M · in vitro · 4 daysAs given in this study (bovine).
Source sentences
Summary
However, neither kisspeptin-10 nor kisspeptin antagonist affected steroid secretion or viable cell number in any of the four ovarian cell culture models.
1 context sentence(design, population, supporting numbers)
Summary · context
Bovine TC and GC cultured under both non-luteinizing (serum-free) and luteinizing (serum-supplemented) conditions were treated for 4 days with kisspeptin-10 (10-10-10-6M) or kisspeptin antagonist (p234; 10-10-10-6M), alone and in combination with either FSH (GC), LH (TC) or forskolin (luteinized GC/TC).
Show in the study text Report a problemFND-3674502401
02LabIn vitro (cells)Efficacy outcome
In four bovine ovarian cell culture models, kisspeptin‑10 did not affect viable cell number.
In plain wordsIn cow ovary cells grown in a dish, kisspeptin‑10 did not change the number of living cells.
Peptide
Kisspeptin-10
Outcome
No significant changeViable cell number of cultured ovarian cells
Study
bovinen: not reported in source
cultured bovine granulosa and theca interna cells, non-luteinizing and luteinizing conditions
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
10-10-10-6M · in vitro · 4 daysAs given in this study (bovine).
Source sentences
Summary
However, neither kisspeptin-10 nor kisspeptin antagonist affected steroid secretion or viable cell number in any of the four ovarian cell culture models.
1 context sentence(design, population, supporting numbers)
Summary · context
Bovine TC and GC cultured under both non-luteinizing (serum-free) and luteinizing (serum-supplemented) conditions were treated for 4 days with kisspeptin-10 (10-10-10-6M) or kisspeptin antagonist (p234; 10-10-10-6M), alone and in combination with either FSH (GC), LH (TC) or forskolin (luteinized GC/TC).
Show in the study text Report a problemFND-3674502402

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

03LabEndogenous levelEx vivo tissueOther
In bovine theca interna, granulosa cells and corpora lutea, the body's own KISS1 and KISS1R transcripts were detected, with differences between follicle categories and CL stages.
In plain wordsIn cow ovary tissue, the body's own kisspeptin and its receptor genes were active and differed by follicle size and corpus luteum stage.
Peptide
Kisspeptin-10
Outcome
Not applicableKISS1 and KISS1R transcripts in bovine ovary
Study
bovinen: not reported in source
bovine granulosa and theca interna cells from antral follicles, and corpora lutea
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
KISS1 and KISS1R transcripts were detected in TC, GC and CL with significant differences between follicle categories and CL stages.
1 context sentence(design, population, supporting numbers)
Summary · context
Granulosa (GC) and theca interna (TC) were collected from antral follicles (3-18 mm) categorized into five class sizes.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-3674502403

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

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Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

Subjects

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Publication types

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