Cryoprotection of humanin-like peptides in seminal plasma for ejaculated spermatozoa of crossbred bulls.

Pande M , Ghosh SK , Tyagi S , Katiyar R , Srivastava N , Karkalan M , Kumar S , Krishnappa K , Sirohi AS , Sarika EY , Mira A

Cryo letters1 Sept 2022Cited by 3 (Europe PMC)

PMID36626132DOI10.54680/fr22510110712PepBankPUB-36626132

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

No result from this study comes from a test of the peptide.

Natural levels measured10

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01AnimalEndogenous levelAnimal study (in vivo)Other
In crossbred bulls, seminal plasma humanin ranged from undetectable to 67.6 pg/mL, with a median of 35.2 pg/mL.
In plain wordsIn bulls, the body's own humanin in semen fluid varied widely, with a middle value of 35.2 pg/mL.
Peptide
Humanin
Outcome
Not applicableSeminal plasma humanin concentration
Study
Bovine (crossbred Frieswal bulls)n = 40
Ejaculated semen samples and seminal plasma
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Measurements
Median SP-HN
35.2 pg/mL
seminal plasma
Maximum SP-HN
67.6 pg/mL
seminal plasma
Source sentences
Summary
SP-HN concentration ranged from undetectable to 67.6 pg/mL with a median level of 35.2 pg/mL.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of 40 semen samples were separated into two groups based on the initial progressive motility (IPM): Good (IPM >70%) and Poor (IPM 50%) and Non-freezable (PTM < 50%) groups.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3662613201
02AnimalEndogenous levelAnimal study (in vivo)Other
In crossbred bulls, seminal plasma humanin was significantly higher in good quality semen than in poor quality semen.
In plain wordsIn bulls, the body's own humanin was higher in good quality semen than in poor quality semen.
Peptide
Humanin
Outcome
IncreaseHumanin in good versus poor quality semen
Study
Bovine (crossbred Frieswal bulls)n = 40
Ejaculated semen samples and seminal plasma
Comparator
Poor quality semen group (IPM <50%)
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
SP-HN level was significantly higher in the good quality semen group than in the poor quality semen group (p<0.001)
1 context sentence(design, population, supporting numbers)
Summary · context
A total of 40 semen samples were separated into two groups based on the initial progressive motility (IPM): Good (IPM >70%) and Poor (IPM 50%) and Non-freezable (PTM < 50%) groups.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3662613202
03AnimalEndogenous levelAnimal study (in vivo)Other
In crossbred bulls, seminal plasma humanin was significantly higher in freezable semen than in non-freezable semen.
In plain wordsIn bulls, the body's own humanin was higher in semen that survives freezing than in semen that does not.
Peptide
Humanin
Outcome
IncreaseHumanin in freezable versus non-freezable semen
Study
Bovine (crossbred Frieswal bulls)n = 40
Ejaculated semen samples and seminal plasma
Comparator
Non-freezable group (PTM < 50%)
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
and also significantly higher in the freezable group than in the non-freezable group (p<0.001).
1 context sentence(design, population, supporting numbers)
Summary · context
A total of 40 semen samples were separated into two groups based on the initial progressive motility (IPM): Good (IPM >70%) and Poor (IPM 50%) and Non-freezable (PTM < 50%) groups.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3662613203
04AnimalEndogenous levelAnimal study (in vivo)Other
In crossbred bulls, seminal plasma humanin was positively correlated with initial progressive motility and post-thaw semen motility.
In plain wordsIn bulls, more of the body's own humanin in semen was linked to better sperm movement before and after freezing.
Peptide
Humanin
Outcome
IncreaseHumanin correlation with initial progressive motility and post-thaw motility
Study
Bovine (crossbred Frieswal bulls)n = 40
Ejaculated semen samples and seminal plasma
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
SP-HN level was positively correlated with initial progressive motility, post-thaw semen motility, viability, acrosome intactness and plasma membrane integrity, but negatively correlated the level of reactive oxygen species and malondialdehyde content.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of 40 semen samples were separated into two groups based on the initial progressive motility (IPM): Good (IPM >70%) and Poor (IPM 50%) and Non-freezable (PTM < 50%) groups.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3662613204
05AnimalEndogenous levelAnimal study (in vivo)Other
In crossbred bulls, seminal plasma humanin was positively correlated with sperm viability.
In plain wordsIn bulls, more of the body's own humanin in semen was linked to more living sperm.
Peptide
Humanin
Outcome
IncreaseHumanin correlation with sperm viability
Study
Bovine (crossbred Frieswal bulls)n = 40
Ejaculated semen samples and seminal plasma
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
SP-HN level was positively correlated with initial progressive motility, post-thaw semen motility, viability, acrosome intactness and plasma membrane integrity, but negatively correlated the level of reactive oxygen species and malondialdehyde content.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of 40 semen samples were separated into two groups based on the initial progressive motility (IPM): Good (IPM >70%) and Poor (IPM 50%) and Non-freezable (PTM < 50%) groups.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3662613205
06AnimalEndogenous levelAnimal study (in vivo)Other
In crossbred bulls, seminal plasma humanin was positively correlated with acrosome intactness.
In plain wordsIn bulls, more of the body's own humanin in semen was linked to more intact sperm caps.
Peptide
Humanin
Outcome
IncreaseHumanin correlation with acrosome intactness
Study
Bovine (crossbred Frieswal bulls)n = 40
Ejaculated semen samples and seminal plasma
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
SP-HN level was positively correlated with initial progressive motility, post-thaw semen motility, viability, acrosome intactness and plasma membrane integrity, but negatively correlated the level of reactive oxygen species and malondialdehyde content.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of 40 semen samples were separated into two groups based on the initial progressive motility (IPM): Good (IPM >70%) and Poor (IPM 50%) and Non-freezable (PTM < 50%) groups.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3662613206
07AnimalEndogenous levelAnimal study (in vivo)Other
In crossbred bulls, seminal plasma humanin was positively correlated with sperm plasma membrane integrity.
In plain wordsIn bulls, more of the body's own humanin in semen was linked to more intact sperm membranes.
Peptide
Humanin
Outcome
IncreaseHumanin correlation with plasma membrane integrity
Study
Bovine (crossbred Frieswal bulls)n = 40
Ejaculated semen samples and seminal plasma
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
SP-HN level was positively correlated with initial progressive motility, post-thaw semen motility, viability, acrosome intactness and plasma membrane integrity, but negatively correlated the level of reactive oxygen species and malondialdehyde content.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of 40 semen samples were separated into two groups based on the initial progressive motility (IPM): Good (IPM >70%) and Poor (IPM 50%) and Non-freezable (PTM < 50%) groups.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3662613207
08AnimalEndogenous levelAnimal study (in vivo)Other
In crossbred bulls, seminal plasma humanin was negatively correlated with the level of reactive oxygen species.
In plain wordsIn bulls, more of the body's own humanin in semen was linked to lower levels of reactive oxygen species.
Peptide
Humanin
Outcome
DecreaseHumanin correlation with reactive oxygen species
Study
Bovine (crossbred Frieswal bulls)n = 40
Ejaculated semen samples and seminal plasma
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
SP-HN level was positively correlated with initial progressive motility, post-thaw semen motility, viability, acrosome intactness and plasma membrane integrity, but negatively correlated the level of reactive oxygen species and malondialdehyde content.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of 40 semen samples were separated into two groups based on the initial progressive motility (IPM): Good (IPM >70%) and Poor (IPM 50%) and Non-freezable (PTM < 50%) groups.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3662613208
09AnimalEndogenous levelAnimal study (in vivo)Other
In crossbred bulls, seminal plasma humanin was negatively correlated with malondialdehyde content.
In plain wordsIn bulls, more of the body's own humanin in semen was linked to lower malondialdehyde, a marker of oxidative damage.
Peptide
Humanin
Outcome
DecreaseHumanin correlation with malondialdehyde
Study
Bovine (crossbred Frieswal bulls)n = 40
Ejaculated semen samples and seminal plasma
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
SP-HN level was positively correlated with initial progressive motility, post-thaw semen motility, viability, acrosome intactness and plasma membrane integrity, but negatively correlated the level of reactive oxygen species and malondialdehyde content.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of 40 semen samples were separated into two groups based on the initial progressive motility (IPM): Good (IPM >70%) and Poor (IPM 50%) and Non-freezable (PTM < 50%) groups.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3662613209
10AnimalEndogenous levelAnimal study (in vivo)Other
In bull spermatozoa, immunochemical localization showed humanin in the proximal region of the middle piece.
In plain wordsIn bull sperm, the body's own humanin was found in the first part of the middle section.
Peptide
Humanin
Outcome
Not applicableHumanin localization in spermatozoa
Study
Bovine (crossbred Frieswal bulls)n = 40
Ejaculated semen samples and seminal plasma
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Immunochemical localization showed the presence of humanin in the proximal region of the middle piece of spermatozoa.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of 40 semen samples were separated into two groups based on the initial progressive motility (IPM): Good (IPM >70%) and Poor (IPM 50%) and Non-freezable (PTM < 50%) groups.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3662613210

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

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