Nicotinamide Mononucleotide Ameliorates Silica-Induced Lung Injury through the Nrf2-Regulated Glutathione Metabolism Pathway in Mice.

Wang L, Zhao M, Qian R, Wang M, Bao Q, Chen X, Du W, Zhang L, Ye T, Xie Y, Zhang B, Peng L, Yao Y

Nutrients28 Dec 2022Cited by 15 (Europe PMC)

PMID36615800PMCIDPMC9823503DOI10.3390/nu15010143PepBankPUB-36615800

Summary

Nicotinamide mononucleotide (NMN) is a natural antioxidant approved as a nutritional supplement and food ingredient, but its protective role in silicosis characterized by oxidative damage remains unknown. In this study, we generated a silicosis model by intratracheal instillation of silica, and then performed histopathological, biochemical, and transcriptomic analysis to evaluate the role of NMN in silicosis. We found that NMN mitigated lung damage at 7 and 28 days, manifested as a decreasing coefficient of lung weight and histological changes, and alleviated oxidative damage by reducing levels of reactive oxygen species and increasing glutathione. Meanwhile, NMN treatment also reduced the recruitment of inflammatory cells and inflammatory infiltration in lung tissue. Transcriptomic analysis showed that NMN treatment mainly regulated immune response and glutathione metabolism pathways. Additionally, NMN upregulated the expression of antioxidant genes Gstm1, Gstm2, and Mgst1 by promoting the expression and nuclear translocation of nuclear factor-erythroid 2 related factor 2 (Nrf2). Gene interaction analysis showed that Nrf2 interacted with Gstm1 and Mgst1 through Gtsm2. Promisingly, oxidative damage mediated by these genes occurred mainly in fibroblasts. In summary, NMN alleviates silica-induced oxidative stress and lung injury by regulating the endogenous glutathione metabolism pathways. This study reveals that NMN supplementation might be a promising strategy for mitigating oxidative stress and inflammation in silicosis.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study15

13 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In silica-exposed mice, NMN decreased lung weight coefficient at 7 and 28 days.
In plain wordsIn mice exposed to silica, NMN decreased lung weight coefficient at 7 and 28 days.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseLung weight coefficient
Study
mousen: not reported in source
Silica-induced lung injury
Outcome group
Lung injury and fibrosis · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
We found that NMN mitigated lung damage at 7 and 28 days, manifested as a decreasing coefficient of lung weight and histological changes, and alleviated oxidative damage by reducing levels of reactive oxygen species and increasing glutathione.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we generated a silicosis model by intratracheal instillation of silica, and then performed histopathological, biochemical, and transcriptomic analysis to evaluate the role of NMN in silicosis.
Show in the study text Report a problemFND-3661580001
02AnimalAnimal study (in vivo)Efficacy outcome
In silica-exposed mice, NMN mitigated lung histological damage at 7 and 28 days.
In plain wordsIn mice exposed to silica, NMN reduced lung tissue damage at 7 and 28 days.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseLung histological injury
Study
mousen: not reported in source
Silica-induced lung injury
Outcome group
Lung injury and fibrosis · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
We found that NMN mitigated lung damage at 7 and 28 days, manifested as a decreasing coefficient of lung weight and histological changes, and alleviated oxidative damage by reducing levels of reactive oxygen species and increasing glutathione.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we generated a silicosis model by intratracheal instillation of silica, and then performed histopathological, biochemical, and transcriptomic analysis to evaluate the role of NMN in silicosis.
Show in the study text Report a problemFND-3661580002
03AnimalAnimal study (in vivo)Efficacy outcome
In silica-exposed mice, NMN reduced reactive oxygen species levels.
In plain wordsIn mice exposed to silica, NMN reduced reactive oxygen species levels.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseReactive oxygen species
Study
mousen: not reported in source
Silica-induced lung injury
Outcome group
Oxidative stress · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
We found that NMN mitigated lung damage at 7 and 28 days, manifested as a decreasing coefficient of lung weight and histological changes, and alleviated oxidative damage by reducing levels of reactive oxygen species and increasing glutathione.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we generated a silicosis model by intratracheal instillation of silica, and then performed histopathological, biochemical, and transcriptomic analysis to evaluate the role of NMN in silicosis.
Show in the study text Report a problemFND-3661580003
04AnimalAnimal study (in vivo)Efficacy outcome
In silica-exposed mice, NMN increased glutathione.
In plain wordsIn mice exposed to silica, NMN increased glutathione.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseGlutathione
Study
mousen: not reported in source
Silica-induced lung injury
Outcome group
Oxidative stress · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
We found that NMN mitigated lung damage at 7 and 28 days, manifested as a decreasing coefficient of lung weight and histological changes, and alleviated oxidative damage by reducing levels of reactive oxygen species and increasing glutathione.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we generated a silicosis model by intratracheal instillation of silica, and then performed histopathological, biochemical, and transcriptomic analysis to evaluate the role of NMN in silicosis.
Show in the study text Report a problemFND-3661580004
05AnimalAnimal study (in vivo)Efficacy outcome
In silica-exposed mice, NMN reduced inflammatory cell recruitment.
In plain wordsIn mice exposed to silica, NMN reduced recruitment of inflammatory cells.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseInflammatory cell recruitment
Study
mousen: not reported in source
Silica-induced lung injury
Outcome group
Immune response · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
Meanwhile, NMN treatment also reduced the recruitment of inflammatory cells and inflammatory infiltration in lung tissue.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we generated a silicosis model by intratracheal instillation of silica, and then performed histopathological, biochemical, and transcriptomic analysis to evaluate the role of NMN in silicosis.
Show in the study text Report a problemFND-3661580005
06AnimalAnimal study (in vivo)Efficacy outcome
In silica-exposed mice, NMN reduced inflammatory infiltration in lung tissue.
In plain wordsIn mice exposed to silica, NMN reduced inflammatory infiltration in lung tissue.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseLung inflammatory infiltration
Study
mousen: not reported in source
Silica-induced lung injury
Outcome group
Inflammation markers · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
Meanwhile, NMN treatment also reduced the recruitment of inflammatory cells and inflammatory infiltration in lung tissue.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we generated a silicosis model by intratracheal instillation of silica, and then performed histopathological, biochemical, and transcriptomic analysis to evaluate the role of NMN in silicosis.
Show in the study text Report a problemFND-3661580006
07AnimalAnimal study (in vivo)Mechanism
In silica-exposed mice, NMN increased Gstm1 expression.
In plain wordsIn mice exposed to silica, NMN increased Gstm1 expression.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseGstm1 expression
Study
mousen: not reported in source
Silica-induced lung injury
Outcome group
Receptor activity and mechanism · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
Additionally, NMN upregulated the expression of antioxidant genes Gstm1, Gstm2, and Mgst1 by promoting the expression and nuclear translocation of nuclear factor-erythroid 2 related factor 2 (Nrf2).
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we generated a silicosis model by intratracheal instillation of silica, and then performed histopathological, biochemical, and transcriptomic analysis to evaluate the role of NMN in silicosis.
Show in the study text Report a problemFND-3661580009
08AnimalAnimal study (in vivo)Mechanism
In silica-exposed mice, NMN increased Gstm2 expression.
In plain wordsIn mice exposed to silica, NMN increased Gstm2 expression.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseGstm2 expression
Study
mousen: not reported in source
Silica-induced lung injury
Outcome group
Receptor activity and mechanism · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
Additionally, NMN upregulated the expression of antioxidant genes Gstm1, Gstm2, and Mgst1 by promoting the expression and nuclear translocation of nuclear factor-erythroid 2 related factor 2 (Nrf2).
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we generated a silicosis model by intratracheal instillation of silica, and then performed histopathological, biochemical, and transcriptomic analysis to evaluate the role of NMN in silicosis.
Show in the study text Report a problemFND-3661580010
09AnimalAnimal study (in vivo)Mechanism
In silica-exposed mice, NMN increased Mgst1 expression.
In plain wordsIn mice exposed to silica, NMN increased Mgst1 expression.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseMgst1 expression
Study
mousen: not reported in source
Silica-induced lung injury
Outcome group
Receptor activity and mechanism · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
Additionally, NMN upregulated the expression of antioxidant genes Gstm1, Gstm2, and Mgst1 by promoting the expression and nuclear translocation of nuclear factor-erythroid 2 related factor 2 (Nrf2).
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we generated a silicosis model by intratracheal instillation of silica, and then performed histopathological, biochemical, and transcriptomic analysis to evaluate the role of NMN in silicosis.
Show in the study text Report a problemFND-3661580011
10AnimalAnimal study (in vivo)Mechanism
In silica-exposed mice, NMN promoted Nrf2 expression.
In plain wordsIn mice exposed to silica, NMN increased Nrf2 expression.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseNrf2 expression
Study
mousen: not reported in source
Silica-induced lung injury
Outcome group
Receptor activity and mechanism · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
Additionally, NMN upregulated the expression of antioxidant genes Gstm1, Gstm2, and Mgst1 by promoting the expression and nuclear translocation of nuclear factor-erythroid 2 related factor 2 (Nrf2).
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we generated a silicosis model by intratracheal instillation of silica, and then performed histopathological, biochemical, and transcriptomic analysis to evaluate the role of NMN in silicosis.
Show in the study text Report a problemFND-3661580012
11AnimalAnimal study (in vivo)Mechanism
In silica-exposed mice, NMN promoted Nrf2 nuclear translocation.
In plain wordsIn mice exposed to silica, NMN increased movement of Nrf2 into the nucleus.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseNrf2 nuclear translocation
Study
mousen: not reported in source
Silica-induced lung injury
Outcome group
Receptor activity and mechanism · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
Additionally, NMN upregulated the expression of antioxidant genes Gstm1, Gstm2, and Mgst1 by promoting the expression and nuclear translocation of nuclear factor-erythroid 2 related factor 2 (Nrf2).
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we generated a silicosis model by intratracheal instillation of silica, and then performed histopathological, biochemical, and transcriptomic analysis to evaluate the role of NMN in silicosis.
Show in the study text Report a problemFND-3661580013
12Computer modelIn silicoMechanism
In silica-exposed mice, transcriptomic analysis indicated that NMN regulated immune response pathways.
In plain wordsIn mice exposed to silica, gene-expression analysis showed that NMN regulated immune response pathways.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Direction not reportedImmune response pathways
Study
mousen: not reported in source
Transcriptomic pathway analysis of the mouse silicosis model
Outcome group
Immune response · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
Transcriptomic analysis showed that NMN treatment mainly regulated immune response and glutathione metabolism pathways.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we generated a silicosis model by intratracheal instillation of silica, and then performed histopathological, biochemical, and transcriptomic analysis to evaluate the role of NMN in silicosis.
Show in the study text Report a problemFND-3661580007
13Computer modelIn silicoMechanism
In silica-exposed mice, transcriptomic analysis indicated that NMN regulated glutathione metabolism pathways.
In plain wordsIn mice exposed to silica, gene-expression analysis showed that NMN regulated glutathione metabolism pathways.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Direction not reportedGlutathione metabolism pathways
Study
mousen: not reported in source
Transcriptomic pathway analysis of the mouse silicosis model
Outcome group
Receptor activity and mechanism · Respiratory
Studied dose
Dose not stated
Source sentences
Summary
Transcriptomic analysis showed that NMN treatment mainly regulated immune response and glutathione metabolism pathways.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, we generated a silicosis model by intratracheal instillation of silica, and then performed histopathological, biochemical, and transcriptomic analysis to evaluate the role of NMN in silicosis.
Show in the study text Report a problemFND-3661580008

Studies that did not test the peptide alone2

In this study, NMN (nicotinamide mononucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking NMN (nicotinamide mononucleotide).

14Computer modelNot a test of NMN (nicotinamide mononucleotide) aloneIn silicoMechanism
In gene interaction analysis from the NMN silicosis study, Nrf2 interacted with Gstm1 and Mgst1 through Gtsm2.
In plain wordsComputer analysis in the NMN study linked Nrf2 with Gstm1 and Mgst1 through Gtsm2.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Not applicableNrf2 gene interactions
Study
n: not reported in source
Gene interaction analysis
Outcome group
Receptor activity and mechanism · Respiratory
Source sentences
Summary
Gene interaction analysis showed that Nrf2 interacted with Gstm1 and Mgst1 through Gtsm2.
In this study, NMN (nicotinamide mononucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NMN (nicotinamide mononucleotide).
Show in the study text Report a problemFND-3661580014
15Computer modelNot a test of NMN (nicotinamide mononucleotide) aloneIn silicoMechanism
In the NMN silicosis study, oxidative damage mediated by the identified genes occurred mainly in fibroblasts.
In plain wordsComputer analysis in the NMN study localized gene-mediated oxidative damage mainly to fibroblasts.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Not applicableFibroblast localization of oxidative damage
Study
n: not reported in source
Cell-type analysis of oxidative damage genes
Outcome group
Receptor activity and mechanism · Respiratory
Source sentences
Summary
Promisingly, oxidative damage mediated by these genes occurred mainly in fibroblasts.
1 context sentence(design, population, supporting numbers)
Summary · context
Gene interaction analysis showed that Nrf2 interacted with Gstm1 and Mgst1 through Gtsm2.
In this study, NMN (nicotinamide mononucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NMN (nicotinamide mononucleotide).
Show in the study text Report a problemFND-3661580015

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

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Terms as the source lists them.

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