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Study · Animal study (in vivo) · mouse · 2023

4 results

Elamipretide alleviates pyroptosis in traumatically injured spinal cord by inhibiting cPLA2-induced lysosomal membrane permeabilization.

Zhang H, Chen Y, Li F, Wu C, Cai W, Ye H, Su H, He M, Yang L, Wang X, Zhou K, Ni W

Journal of neuroinflammation7 Jan 2023Cited by 40 (Europe PMC)

PMID36609266 (opens in a new tab)PMCIDPMC9825014 (opens in a new tab)DOI10.1186/s12974-023-02690-4 (opens in a new tab)PepBankPUB-36609266
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Summary

Spinal cord injury (SCI) is a devastating injury that may result in permanent motor impairment. The active ingredients of medications are unable to reach the affected area due to the blood‒brain barrier. Elamipretide (SS-31) is a new and innovative aromatic cationic peptide. Because of its alternating aromatic and cationic groups, it freely crosses the blood‒brain barrier. It is also believed to decrease inflammation and protect against a variety of neurological illnesses. This study explored the therapeutic value of SS-31 in functional recovery after SCI and its possible underlying mechanism. A spinal cord contusion injury model as well as the Basso Mouse Scale, footprint assessment, and inclined plane test were employed to assess how well individuals could function following SCI. The area of glial scarring, the number of dendrites, and the number of synapses after SCI were confirmed by HE, Masson, MAP2, and Syn staining. Western blotting, immunofluorescence, and enzyme-linked immunosorbent assays were employed to examine the expression levels of pyroptosis-, autophagy-, lysosomal membrane permeabilization (LMP)- and MAPK signalling-related proteins. The outcomes showed that SS-31 inhibited pyroptosis, enhanced autophagy and attenuated LMP in SCI. Mechanistically, we applied AAV vectors to upregulate Pla2g4A in vivo and found that SS-31 enhanced autophagy and attenuated pyroptosis and LMP by inhibiting phosphorylation of cPLA2. Ultimately, we applied asiatic acid (a p38-MAPK agonist) to test whether SS-31 regulated cPLA2 partially through the MAPK-P38 signalling pathway. Our group is the first to suggest that SS-31 promotes functional recovery partially by inhibiting cPLA2-mediated autophagy impairment and preventing LMP and pyroptosis after SCI, which may have potential clinical application value.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Mechanism

    In mice with spinal cord contusion injury, SS‑31 inhibited pyroptosis.

    In plain wordsIn mice with spinal cord injury, SS‑31 reduced a form of inflammatory cell death (pyroptosis).

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreasePyroptosis after spinal cord injury
    Study

    mousen: not reported in source

    Spinal cord contusion injury
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The outcomes showed that SS-31 inhibited pyroptosis, enhanced autophagy and attenuated LMP in SCI.
    1 context sentence
    Summary · context
    A spinal cord contusion injury model as well as the Basso Mouse Scale, footprint assessment, and inclined plane test were employed to assess how well individuals could function following SCI.
  2. 02AnimalAnimal study (in vivo)Mechanism

    In mice with spinal cord contusion injury, SS‑31 enhanced autophagy.

    In plain wordsIn mice with spinal cord injury, SS‑31 increased the cell clean-up process called autophagy.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseAutophagy after spinal cord injury
    Study

    mousen: not reported in source

    Spinal cord contusion injury
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The outcomes showed that SS-31 inhibited pyroptosis, enhanced autophagy and attenuated LMP in SCI.
    1 context sentence
    Summary · context
    A spinal cord contusion injury model as well as the Basso Mouse Scale, footprint assessment, and inclined plane test were employed to assess how well individuals could function following SCI.
  3. 03AnimalAnimal study (in vivo)Mechanism

    In mice with spinal cord contusion injury, SS‑31 attenuated lysosomal membrane permeabilization.

    In plain wordsIn mice with spinal cord injury, SS‑31 reduced leakage from the cell's lysosomes.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseLysosomal membrane permeabilization (LMP) after spinal cord injury
    Study

    mousen: not reported in source

    Spinal cord contusion injury
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The outcomes showed that SS-31 inhibited pyroptosis, enhanced autophagy and attenuated LMP in SCI.
    1 context sentence
    Summary · context
    A spinal cord contusion injury model as well as the Basso Mouse Scale, footprint assessment, and inclined plane test were employed to assess how well individuals could function following SCI.
  4. 04AnimalAnimal study (in vivo)Mechanism

    In mice with spinal cord contusion injury, SS‑31 inhibited phosphorylation of cPLA2, which mediated its effects on autophagy, pyroptosis and LMP.

    In plain wordsIn mice with spinal cord injury, SS‑31 lowered activation of the enzyme cPLA2, linked to its other effects.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreasecPLA2 phosphorylation
    Study

    mousen: not reported in source

    Spinal cord contusion injury
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Mechanistically, we applied AAV vectors to upregulate Pla2g4A in vivo and found that SS-31 enhanced autophagy and attenuated pyroptosis and LMP by inhibiting phosphorylation of cPLA2.
    1 context sentence
    Summary · context
    A spinal cord contusion injury model as well as the Basso Mouse Scale, footprint assessment, and inclined plane test were employed to assess how well individuals could function following SCI.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    9 Oct 2026, 18:17 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:47 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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