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Study · Animal study (in vivo) · mouse · 2022

12 results

L-carnosine Attenuates Bleomycin-Induced Oxidative Stress via NFκB Pathway in the Pathogenesis of Pulmonary Fibrosis.

Park J, Jang J, Cha SR, Baek H, Lee J, Hong SH, Lee HA, Lee TJ, Yang SR

Antioxidants (Basel, Switzerland)14 Dec 2022Cited by 18 (Europe PMC)

PMID36552670 (opens in a new tab)PMCIDPMC9774395 (opens in a new tab)DOI10.3390/antiox11122462 (opens in a new tab)PepBankPUB-36552670
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Summary

Idiopathic Pulmonary fibrosis (IPF), a chronic interstitial lung disease, has pulmonary manifestations clinically characterized by collagen deposition, epithelial cell injury, and a decline in lung function. L-carnosine, a dipeptide consisting of β-alanine and L-histidine, has demonstrated a therapeutic effect on various diseases because of its pivotal function. Despite the effect of L-carnosine in experimental IPF mice, its anti-oxidative effect and associated intercellular pathway, particularly alveolar epithelial cells, remain unknown. Therefore, we demonstrated the anti-fibrotic and anti-inflammatory effects of L-carnosine via Reactive oxygen species (ROS) regulation in bleomycin (BLM)-induced IPF mice. The mice were intratracheally injected with BLM (3 mg/kg) and L-carnosine (150 mg/kg) was orally administrated for 2 weeks. BLM exposure increased the protein level of Nox2, Nox4, p53, and Caspase-3, whereas L-carnosine treatment suppressed the protein level of Nox2, Nox4, p53, and Caspase-3 cleavage in mice. In addition, the total SOD activity and mRNA level of Sod2, catalase, and Nqo1 increased in mice treated with L-carnosine. At the cellular level, a human fibroblast (MRC-5) and mouse alveolar epithelial cell (MLE-12) were exposed to TGFβ1 following L-carnosine treatment to induce fibrogenesis. Moreover, MLE-12 cells were exposed to cigarette smoke extract (CSE). Consequently, L-carnosine treatment ameliorated fibrogenesis in fibroblasts and alveolar epithelial cells, and inflammation induced by ROS and CSE exposure was ameliorated. These results were associated with the inhibition of the NFκB pathway. Collectively, our data indicate that L-carnosine induces anti-inflammatory and anti-fibrotic effects on alveolar epithelial cells against the pathogenesis of IPF.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study12

12 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Mechanism

    In bleomycin-exposed mice, L‑carnosine suppressed Nox2 protein levels.

    In plain wordsIn mice exposed to bleomycin, L‑carnosine reduced Nox2 protein levels.

    Peptide
    L-carnosine
    Outcome
    DecreaseNox2 protein
    Study

    mousen: not reported in source

    Bleomycin-induced pulmonary fibrosis
    Outcome group
    Oxidative stress · Respiratory
    Studied dose
    150 mg/kg · oral · 2 weeksAs given in this study (mouse).

    Source sentences

    Summary
    BLM exposure increased the protein level of Nox2, Nox4, p53, and Caspase-3, whereas L-carnosine treatment suppressed the protein level of Nox2, Nox4, p53, and Caspase-3 cleavage in mice.
    1 context sentence
    Summary · context
    The mice were intratracheally injected with BLM (3 mg/kg) and L-carnosine (150 mg/kg) was orally administrated for 2 weeks.
  2. 02AnimalAnimal study (in vivo)Mechanism

    In bleomycin-exposed mice, L‑carnosine suppressed Nox4 protein levels.

    In plain wordsIn mice exposed to bleomycin, L‑carnosine reduced Nox4 protein levels.

    Peptide
    L-carnosine
    Outcome
    DecreaseNox4 protein
    Study

    mousen: not reported in source

    Bleomycin-induced pulmonary fibrosis
    Outcome group
    Oxidative stress · Respiratory
    Studied dose
    150 mg/kg · oral · 2 weeksAs given in this study (mouse).

    Source sentences

    Summary
    BLM exposure increased the protein level of Nox2, Nox4, p53, and Caspase-3, whereas L-carnosine treatment suppressed the protein level of Nox2, Nox4, p53, and Caspase-3 cleavage in mice.
    1 context sentence
    Summary · context
    The mice were intratracheally injected with BLM (3 mg/kg) and L-carnosine (150 mg/kg) was orally administrated for 2 weeks.
  3. 03AnimalAnimal study (in vivo)Mechanism

    In bleomycin-exposed mice, L‑carnosine suppressed p53 protein levels.

    In plain wordsIn mice exposed to bleomycin, L‑carnosine reduced p53 protein levels.

    Peptide
    L-carnosine
    Outcome
    Decreasep53 protein
    Study

    mousen: not reported in source

    Bleomycin-induced pulmonary fibrosis
    Outcome group
    Receptor activity and mechanism · Respiratory
    Studied dose
    150 mg/kg · oral · 2 weeksAs given in this study (mouse).

    Source sentences

    Summary
    BLM exposure increased the protein level of Nox2, Nox4, p53, and Caspase-3, whereas L-carnosine treatment suppressed the protein level of Nox2, Nox4, p53, and Caspase-3 cleavage in mice.
    1 context sentence
    Summary · context
    The mice were intratracheally injected with BLM (3 mg/kg) and L-carnosine (150 mg/kg) was orally administrated for 2 weeks.
  4. 04AnimalAnimal study (in vivo)Mechanism

    In bleomycin-exposed mice, L‑carnosine suppressed Caspase-3 cleavage.

    In plain wordsIn mice exposed to bleomycin, L‑carnosine reduced Caspase-3 cleavage.

    Peptide
    L-carnosine
    Outcome
    DecreaseCaspase-3 cleavage
    Study

    mousen: not reported in source

    Bleomycin-induced pulmonary fibrosis
    Outcome group
    Receptor activity and mechanism · Respiratory
    Studied dose
    150 mg/kg · oral · 2 weeksAs given in this study (mouse).

    Source sentences

    Summary
    BLM exposure increased the protein level of Nox2, Nox4, p53, and Caspase-3, whereas L-carnosine treatment suppressed the protein level of Nox2, Nox4, p53, and Caspase-3 cleavage in mice.
    1 context sentence
    Summary · context
    The mice were intratracheally injected with BLM (3 mg/kg) and L-carnosine (150 mg/kg) was orally administrated for 2 weeks.
  5. 05AnimalAnimal study (in vivo)Mechanism

    In mice, L‑carnosine increased total SOD activity.

    In plain wordsIn mice, L‑carnosine increased total SOD activity.

    Peptide
    L-carnosine
    Outcome
    IncreaseTotal SOD activity
    Study

    mousen: not reported in source

    Bleomycin-induced pulmonary fibrosis
    Outcome group
    Oxidative stress · Respiratory
    Studied dose
    150 mg/kg · oral · 2 weeksAs given in this study (mouse).

    Source sentences

    Summary
    In addition, the total SOD activity and mRNA level of Sod2, catalase, and Nqo1 increased in mice treated with L-carnosine.
    1 context sentence
    Summary · context
    The mice were intratracheally injected with BLM (3 mg/kg) and L-carnosine (150 mg/kg) was orally administrated for 2 weeks.
  6. 06AnimalAnimal study (in vivo)Mechanism

    In mice, L‑carnosine increased Sod2 mRNA levels.

    In plain wordsIn mice, L‑carnosine increased Sod2 gene expression.

    Peptide
    L-carnosine
    Outcome
    IncreaseSod2 mRNA
    Study

    mousen: not reported in source

    Bleomycin-induced pulmonary fibrosis
    Outcome group
    Oxidative stress · Respiratory
    Studied dose
    150 mg/kg · oral · 2 weeksAs given in this study (mouse).

    Source sentences

    Summary
    In addition, the total SOD activity and mRNA level of Sod2, catalase, and Nqo1 increased in mice treated with L-carnosine.
    1 context sentence
    Summary · context
    The mice were intratracheally injected with BLM (3 mg/kg) and L-carnosine (150 mg/kg) was orally administrated for 2 weeks.
  7. 07AnimalAnimal study (in vivo)Mechanism

    In mice, L‑carnosine increased catalase mRNA levels.

    In plain wordsIn mice, L‑carnosine increased catalase gene expression.

    Peptide
    L-carnosine
    Outcome
    IncreaseCatalase mRNA
    Study

    mousen: not reported in source

    Bleomycin-induced pulmonary fibrosis
    Outcome group
    Oxidative stress · Respiratory
    Studied dose
    150 mg/kg · oral · 2 weeksAs given in this study (mouse).

    Source sentences

    Summary
    In addition, the total SOD activity and mRNA level of Sod2, catalase, and Nqo1 increased in mice treated with L-carnosine.
    1 context sentence
    Summary · context
    The mice were intratracheally injected with BLM (3 mg/kg) and L-carnosine (150 mg/kg) was orally administrated for 2 weeks.
  8. 08AnimalAnimal study (in vivo)Mechanism

    In mice, L‑carnosine increased Nqo1 mRNA levels.

    In plain wordsIn mice, L‑carnosine increased Nqo1 gene expression.

    Peptide
    L-carnosine
    Outcome
    IncreaseNqo1 mRNA
    Study

    mousen: not reported in source

    Bleomycin-induced pulmonary fibrosis
    Outcome group
    Oxidative stress · Respiratory
    Studied dose
    150 mg/kg · oral · 2 weeksAs given in this study (mouse).

    Source sentences

    Summary
    In addition, the total SOD activity and mRNA level of Sod2, catalase, and Nqo1 increased in mice treated with L-carnosine.
    1 context sentence
    Summary · context
    The mice were intratracheally injected with BLM (3 mg/kg) and L-carnosine (150 mg/kg) was orally administrated for 2 weeks.
  9. 09LabIn vitro (cells)Efficacy outcome

    In MRC-5 fibroblasts exposed to TGFβ1, L‑carnosine ameliorated fibrogenesis.

    In plain wordsIn cells, L‑carnosine reduced fibrogenesis in human fibroblasts exposed to TGFβ1.

    Peptide
    L-carnosine
    Outcome
    DecreaseFibroblast fibrogenesis
    Study

    humann: not reported in source

    MRC-5 fibroblasts exposed to TGFβ1
    Outcome group
    Lung injury and fibrosis · Respiratory
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Consequently, L-carnosine treatment ameliorated fibrogenesis in fibroblasts and alveolar epithelial cells, and inflammation induced by ROS and CSE exposure was ameliorated.
    1 context sentence
    Summary · context
    At the cellular level, a human fibroblast (MRC-5) and mouse alveolar epithelial cell (MLE-12) were exposed to TGFβ1 following L-carnosine treatment to induce fibrogenesis.
  10. 10LabIn vitro (cells)Efficacy outcome

    In alveolar epithelial cells exposed to TGFβ1, L‑carnosine ameliorated fibrogenesis.

    In plain wordsIn cells, L‑carnosine reduced fibrogenesis in mouse alveolar epithelial cells exposed to TGFβ1.

    Peptide
    L-carnosine
    Outcome
    DecreaseAlveolar epithelial cell fibrogenesis
    Study

    mousen: not reported in source

    MLE-12 alveolar epithelial cells
    Outcome group
    Lung injury and fibrosis · Respiratory
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Consequently, L-carnosine treatment ameliorated fibrogenesis in fibroblasts and alveolar epithelial cells, and inflammation induced by ROS and CSE exposure was ameliorated.
    1 context sentence
    Summary · context
    At the cellular level, a human fibroblast (MRC-5) and mouse alveolar epithelial cell (MLE-12) were exposed to TGFβ1 following L-carnosine treatment to induce fibrogenesis.
  11. 11LabIn vitro (cells)Efficacy outcome

    In cellular experiments, L‑carnosine ameliorated inflammation induced by ROS and cigarette smoke extract exposure.

    In plain wordsIn cells, L‑carnosine reduced inflammation induced by reactive oxygen species and cigarette smoke extract.

    Peptide
    L-carnosine
    Outcome
    DecreaseROS- and CSE-induced inflammation
    Study

    n: not reported in source

    Fibroblasts and alveolar epithelial cells
    Outcome group
    Inflammation markers · Respiratory
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Consequently, L-carnosine treatment ameliorated fibrogenesis in fibroblasts and alveolar epithelial cells, and inflammation induced by ROS and CSE exposure was ameliorated.
    2 context sentences
    Summary · context
    At the cellular level, a human fibroblast (MRC-5) and mouse alveolar epithelial cell (MLE-12) were exposed to TGFβ1 following L-carnosine treatment to induce fibrogenesis.
    Summary · context
    Moreover, MLE-12 cells were exposed to cigarette smoke extract (CSE).
  12. 12LabIn vitro (cells)Mechanism

    In cellular experiments, L‑carnosine effects were associated with NFκB pathway inhibition.

    In plain wordsIn cells, L‑carnosine effects were associated with inhibition of the NFκB pathway.

    Peptide
    L-carnosine
    Outcome
    DecreaseNFκB pathway
    Study

    n: not reported in source

    Fibroblasts and alveolar epithelial cells
    Outcome group
    Receptor activity and mechanism · Respiratory
    Studied dose
    Dose not stated

    Source sentences

    Summary
    These results were associated with the inhibition of the NFκB pathway.
    2 context sentences
    Summary · context
    Consequently, L-carnosine treatment ameliorated fibrogenesis in fibroblasts and alveolar epithelial cells, and inflammation induced by ROS and CSE exposure was ameliorated.
    Summary · context
    At the cellular level, a human fibroblast (MRC-5) and mouse alveolar epithelial cell (MLE-12) were exposed to TGFβ1 following L-carnosine treatment to induce fibrogenesis.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    27 Oct 2026, 18:20 UTC

    Study results updated

  2. Version 1

    27 Oct 2026, 16:32 UTC

    First added · source update from Europe PMC

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