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Study · Animal study (in vivo) · rat · 2022

5 results

Fourier Transform Infrared Spectroscopy Reveals Molecular Changes in Blood Vessels of Rats Treated with Pentadecapeptide BPC 157.

Gamulin O, Oroz K, Coric L, Krajacic M, Skrabic M, Dretar V, Strbe S, Talapko J, Juzbasic M, Krezic I, Lozic M, Stambolija V and 7 more

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Zizek H, Jurca I, Jurjevic I, Blagaic AB, Skrtic A, Seiwerth S, Sikiric P

Biomedicines4 Dec 2022Cited by 19 (Europe PMC)

PMID36551886 (opens in a new tab)PMCIDPMC9775416 (opens in a new tab)DOI10.3390/biomedicines10123130 (opens in a new tab)PepBankPUB-36551886
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Summary

Recently, it was found that when confronted with major vessel occlusion and vascular failure, stable gastric pentadecapeptide BPC 157 therapy might rapidly functionally improve minor vessels to take over the function of disabled major vessels, reorganize blood flow, and compensate failed vessel function. We focused on the BPC 157 therapy effect obtained by giving 10 ng/kg ip to rats 5 min before sacrifice on the rat thoracic aorta, which we assessed with Fourier transform infrared spectroscopy (FTIR) 90 min thereafter. We applied a principal component analysis (PCA). The PCA model showed, with a clear distinction being mostly due to the PC1 score, differences between the spectra of BPC 157- and saline-treated rats. The comparison of the averaged spectra of these two groups with their differential spectrum and PC loadings allowed us to identify the parts of the FTIR spectra that contributed the most to the spectral separation of the two observed groups. The PC1 loadings and the differential spectrum showed that the main bands affecting the separation were the amid I band around 1650 cm-1, the amid II band around 1540 cm-1, and the vibrational band around 1744 cm-1. Fitting the spectral range between 1450 and 1800 cm-1 showed changes in protein conformation and confirmed the appearance of the vibrational band at 1744 cm-1. Controls had a substantially more intense vibrational band at 1744 cm-1. These spectral results showed the cells from saline-treated (control) rats to be in the early stage of cell death, while the samples from BPC 157-rats were protected. Thus, BPC 157 therapy changed the lipid contents and protein secondary structure conformation, with a rapid effect on vessels, within a short time upon application.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Mechanism

    In rat thoracic aorta, principal component analysis showed differences between the FTIR spectra of BPC‑157-treated and saline-treated rats.

    In plain wordsIn rats, the aorta of animals given BPC‑157 showed different infrared readings than the aorta of saline-treated rats.

    Peptide
    BPC-157
    Outcome
    Not applicableFTIR spectral separation (PCA) of aorta
    Study

    ratn: not reported in source

    Rat thoracic aorta, FTIR spectroscopy
    Comparator
    Saline
    Outcome group
    Cardiovascular events · Cardiovascular
    Studied dose
    10 ng/kg · intraperitonealAs given in this study (rat).

    Source sentences

    Summary
    The PCA model showed, with a clear distinction being mostly due to the PC1 score, differences between the spectra of BPC 157- and saline-treated rats.
    2 context sentences
    Title · context
    Fourier Transform Infrared Spectroscopy Reveals Molecular Changes in Blood Vessels of Rats Treated with Pentadecapeptide BPC 157.
    Summary · context
    We focused on the BPC 157 therapy effect obtained by giving 10 ng/kg ip to rats 5 min before sacrifice on the rat thoracic aorta, which we assessed with Fourier transform infrared spectroscopy (FTIR) 90 min thereafter.
  2. 02AnimalAnimal study (in vivo)Mechanism

    In rat thoracic aorta, spectral fitting between 1450 and 1800 cm-1 showed changes in protein conformation; the main separating bands were amide I, amide II and the 1744 cm-1 band.

    In plain wordsIn rats, readings from the aorta showed changes in protein shape linked to BPC‑157 treatment.

    Peptide
    BPC-157
    Outcome
    Not applicableProtein conformation and spectral bands (amide I, amide II, 1744 cm-1)
    Study

    ratn: not reported in source

    Rat thoracic aorta, FTIR spectroscopy
    Comparator
    Saline
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    10 ng/kg · intraperitonealAs given in this study (rat).

    Source sentences

    Summary
    Fitting the spectral range between 1450 and 1800 cm-1 showed changes in protein conformation and confirmed the appearance of the vibrational band at 1744 cm-1.
    2 context sentences
    Summary · context
    The PC1 loadings and the differential spectrum showed that the main bands affecting the separation were the amid I band around 1650 cm-1, the amid II band around 1540 cm-1, and the vibrational band around 1744 cm-1.
    Summary · context
    We focused on the BPC 157 therapy effect obtained by giving 10 ng/kg ip to rats 5 min before sacrifice on the rat thoracic aorta, which we assessed with Fourier transform infrared spectroscopy (FTIR) 90 min thereafter.
  3. 03AnimalAnimal study (in vivo)Mechanism

    In rat thoracic aorta, saline-treated controls had a substantially more intense 1744 cm-1 band than BPC‑157-treated rats.

    In plain wordsIn rats, the aorta of control animals showed a stronger signal at one infrared band than the aorta of BPC‑157 rats.

    Peptide
    BPC-157
    Outcome
    DecreaseIntensity of the 1744 cm-1 vibrational band
    Study

    ratn: not reported in source

    Rat thoracic aorta, FTIR spectroscopy
    Comparator
    Saline
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    10 ng/kg · intraperitonealAs given in this study (rat).

    Source sentences

    Summary
    Controls had a substantially more intense vibrational band at 1744 cm-1.
    2 context sentences
    Title · context
    Fourier Transform Infrared Spectroscopy Reveals Molecular Changes in Blood Vessels of Rats Treated with Pentadecapeptide BPC 157.
    Summary · context
    We focused on the BPC 157 therapy effect obtained by giving 10 ng/kg ip to rats 5 min before sacrifice on the rat thoracic aorta, which we assessed with Fourier transform infrared spectroscopy (FTIR) 90 min thereafter.
  4. 04AnimalAnimal study (in vivo)Mechanism

    In rat thoracic aorta, spectra showed cells from saline-treated rats in the early stage of cell death, while samples from BPC‑157-treated rats were protected.

    In plain wordsIn rats, aorta cells from saline rats looked to be starting to die, while cells from BPC‑157 rats looked protected.

    Peptide
    BPC-157
    Outcome
    DecreaseCell death in aortic tissue
    Study

    ratn: not reported in source

    Rat thoracic aorta, FTIR spectroscopy
    Comparator
    Saline
    Outcome group
    Cardiovascular events · Cardiovascular
    Studied dose
    10 ng/kg · intraperitonealAs given in this study (rat).

    Source sentences

    Summary
    These spectral results showed the cells from saline-treated (control) rats to be in the early stage of cell death, while the samples from BPC 157-rats were protected.
    2 context sentences
    Title · context
    Fourier Transform Infrared Spectroscopy Reveals Molecular Changes in Blood Vessels of Rats Treated with Pentadecapeptide BPC 157.
    Summary · context
    We focused on the BPC 157 therapy effect obtained by giving 10 ng/kg ip to rats 5 min before sacrifice on the rat thoracic aorta, which we assessed with Fourier transform infrared spectroscopy (FTIR) 90 min thereafter.
  5. 05AnimalAnimal study (in vivo)Mechanism

    In rat thoracic aorta, BPC‑157 therapy changed the lipid contents and protein secondary structure conformation within a short time of application.

    In plain wordsIn rats, BPC‑157 changed the fat content and protein shape in the aorta within a short time.

    Peptide
    BPC-157
    Outcome
    Not applicableLipid content and protein secondary structure
    Study

    ratn: not reported in source

    Rat thoracic aorta, FTIR spectroscopy
    Comparator
    Saline
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    10 ng/kg · intraperitonealAs given in this study (rat).

    Source sentences

    Summary
    Thus, BPC 157 therapy changed the lipid contents and protein secondary structure conformation, with a rapid effect on vessels, within a short time upon application.
    2 context sentences
    Title · context
    Fourier Transform Infrared Spectroscopy Reveals Molecular Changes in Blood Vessels of Rats Treated with Pentadecapeptide BPC 157.
    Summary · context
    We focused on the BPC 157 therapy effect obtained by giving 10 ng/kg ip to rats 5 min before sacrifice on the rat thoracic aorta, which we assessed with Fourier transform infrared spectroscopy (FTIR) 90 min thereafter.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    10 Oct 2026, 08:19 UTC

    Study results updated

  2. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 02:56 UTC

    First added · source update from Europe PMC

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