What is the protective effect of preischemic kisspeptin-10 administration against ischemia/reperfusion injury of striatum on mice?

Akkaya H, Sümer E, Kutlu S, Solak H, Yılmaz B

Turkish journal of medical sciences19 Oct 2022Cited by 2 (Europe PMC)

PMID36422497PMCIDPMC10395688DOI10.55730/1300-0144.5493PepBankPUB-36422497

Summary

BackgroundKisspeptin is a neuropeptide with a primary role on the onset of puberty and has beneficial effects on ischemia/ reperfusion (I/R) injury. In this study, we aimed to investigate the effect of kisspeptin administration on striatal I/R injury in mice.MethodsForty adult C57/BL6 mice were randomly divided into four groups: Sham, Kisspeptin, I/R, and I/R + Kisspeptin groups. The groups were administered with either physiological saline (Sham and I/R groups) or kisspeptin (Kisspeptin and I/R + Kisspeptin groups) intraperitoneally 40 min before the operation. A microdialysis probe was placed in the right striatum according to stereotaxic coordinates. During the experimental period, artificial cerebrospinal fluid was passed through the micropump. Then, transient cerebral ischemia was established by compressing both common carotid arteries with an aneurysm clip for 15 min and animals were reperfused for 2 h. Throughout the process of microdialysis (before, during and after I/R period), samples were collected to measure dopamine (DA), noradrenaline (NA), and 3,4-dihydroxyphenylglycine (DHPG) at intervals of 20 min continuously. At the end of the reperfusion period, the animals were decapitated, striatum was dissected, half of the animals were used for oxidative stress analyses (reduced glutathione, glutathione S-transferase (GST), superoxide dismutase (SOD), malondialdehyde (MDA), and the other half were used for histopathology analyses.ResultsNumber of glial cells was significantly increased in kisspeptin-administered groups. DA levels in ischemic animals were decreased by kisspeptin administration (p < 0.0001). NA levels were reduced in animals administered with kisspeptin without I/R injury (p < 0.05). DHPG levels reduced during the reperfusion period in ischemic animals (p < 0.05). Kisspeptin did not exhibit a significant antioxidant activity in the ischemic animals, while GST and SOD levels were reduced in the I/R + kisspeptin group compared to the kisspeptin group (p < 0.05).DiscussionOur results suggest that kisspeptin may be regulating the neurotransmitter release and metabolism, as well as inflammatory response in brain upon I/R injury.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In mice, the number of glial cells was significantly increased in the kisspeptin-administered groups.
In plain wordsIn mice, groups given kisspeptin had more glial cells in the striatum.
Peptide
Kisspeptin-10
Outcome
IncreaseNumber of glial cells
Study
mousen: not reported in source
C57/BL6 mice with striatal ischemia/reperfusion injury
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
Number of glial cells was significantly increased in kisspeptin-administered groups.
2 context sentences(design, population, supporting numbers)
Summary · context
Forty adult C57/BL6 mice were randomly divided into four groups: Sham, Kisspeptin, I/R, and I/R + Kisspeptin groups.
Summary · context
The groups were administered with either physiological saline (Sham and I/R groups) or kisspeptin (Kisspeptin and I/R + Kisspeptin groups) intraperitoneally 40 min before the operation.
Show in the study text Report a problemFND-3642249701
02AnimalAnimal study (in vivo)Efficacy outcome
In mice with striatal ischemia/reperfusion injury, kisspeptin decreased dopamine levels (p < 0.0001).
In plain wordsIn mice with brain ischemia, kisspeptin lowered dopamine levels in the striatum.
Peptide
Kisspeptin-10
Outcome
DecreaseDopamine level in ischemic animals
Study
mousen: not reported in source
C57/BL6 mice with striatal ischemia/reperfusion injury
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
DA levels in ischemic animals were decreased by kisspeptin administration (p < 0.0001).
2 context sentences(design, population, supporting numbers)
Summary · context
Forty adult C57/BL6 mice were randomly divided into four groups: Sham, Kisspeptin, I/R, and I/R + Kisspeptin groups.
Summary · context
The groups were administered with either physiological saline (Sham and I/R groups) or kisspeptin (Kisspeptin and I/R + Kisspeptin groups) intraperitoneally 40 min before the operation.
Show in the study text Report a problemFND-3642249702
03AnimalAnimal study (in vivo)Efficacy outcome
In mice without ischemia/reperfusion injury, kisspeptin reduced noradrenaline levels (p < 0.05).
In plain wordsIn mice without brain injury, kisspeptin lowered noradrenaline levels in the striatum.
Peptide
Kisspeptin-10
Outcome
DecreaseNoradrenaline level without ischemia
Study
mousen: not reported in source
C57/BL6 mice with striatal ischemia/reperfusion injury
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
NA levels were reduced in animals administered with kisspeptin without I/R injury (p < 0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
Forty adult C57/BL6 mice were randomly divided into four groups: Sham, Kisspeptin, I/R, and I/R + Kisspeptin groups.
Summary · context
The groups were administered with either physiological saline (Sham and I/R groups) or kisspeptin (Kisspeptin and I/R + Kisspeptin groups) intraperitoneally 40 min before the operation.
Show in the study text Report a problemFND-3642249703
04AnimalAnimal study (in vivo)Efficacy outcome
In ischemic mice, DHPG levels were reduced during the reperfusion period (p < 0.05).
In plain wordsIn mice with brain ischemia, DHPG levels were lower during reperfusion.
Peptide
Kisspeptin-10
Outcome
Direction not reportedDHPG level during reperfusion
Study
mousen: not reported in source
C57/BL6 mice with striatal ischemia/reperfusion injury
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
DHPG levels reduced during the reperfusion period in ischemic animals (p < 0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
Forty adult C57/BL6 mice were randomly divided into four groups: Sham, Kisspeptin, I/R, and I/R + Kisspeptin groups.
Summary · context
The groups were administered with either physiological saline (Sham and I/R groups) or kisspeptin (Kisspeptin and I/R + Kisspeptin groups) intraperitoneally 40 min before the operation.
Show in the study text Report a problemFND-3642249704
05AnimalAnimal study (in vivo)Efficacy outcome
In mice with striatal ischemia/reperfusion injury, kisspeptin did not show significant antioxidant activity.
In plain wordsIn mice with brain ischemia, kisspeptin did not show a significant antioxidant effect.
Peptide
Kisspeptin-10
Outcome
No significant changeAntioxidant activity in ischemic animals
Study
mousen: not reported in source
C57/BL6 mice with striatal ischemia/reperfusion injury
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
Kisspeptin did not exhibit a significant antioxidant activity in the ischemic animals
2 context sentences(design, population, supporting numbers)
Summary · context
Forty adult C57/BL6 mice were randomly divided into four groups: Sham, Kisspeptin, I/R, and I/R + Kisspeptin groups.
Summary · context
The groups were administered with either physiological saline (Sham and I/R groups) or kisspeptin (Kisspeptin and I/R + Kisspeptin groups) intraperitoneally 40 min before the operation.
Show in the study text Report a problemFND-3642249705

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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