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Study · Not a test of the peptide alone · 2022

7 results

Conjugated PNC-27 peptide/PEI-superparamagnetic iron oxide nanoparticles (SPIONs) as a double targeting agent for early cancer diagnosis: In vitro study.

Rahmani R, Darroudi M, Gharanfoli M, Chamani J, Gholamin M, Hashemi M

Iranian journal of basic medical sciences1 Oct 2022Cited by 2 (Europe PMC)

PMID36311203 (opens in a new tab)PMCIDPMC9588323 (opens in a new tab)DOI10.22038/ijbms.2022.65590.14430 (opens in a new tab)PepBankPUB-36311203
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Summary

ObjectivesSuperparamagnetic iron oxide nanoparticles (SPIONs) have been considered promising non-invasive imaging tools in medicine. However, their high surface energy leads to NPs aggregation, while non-targeted SPIONs can cause cytotoxic effects on normal cells. In this work, we evaluated the in vitro potential of polyethyleneimine (PEI)-SPIONs targeted by PNC-27 peptide as a double targeting agent throughout early cancer diagnosis.Materials and methodsInitially, PEI was conjugated to PNC-27 with HDM-2-binding domain. Then, SPIONs were loaded into PEI-PNC-27 through the ligand exchange method. The physicochemical characteristics of the synthesized NPs were evaluated. The cytotoxicity and targeting efficiency were assayed against HT-29 and CT-26 cell lines along with NIH-3t3 as normal cells by MTT method and Prussian blue staining test, respectively.ResultsThe mean diameter of synthesized carriers was obtained in the range of 86.6 - 116.1 nm with a positive charge. According to the cytotoxicity results, the binding and uptake abilities of the PNC-27 peptide by cancer cells were significantly higher than that of the NIH-3t3 cells. However, the results were indicative of the more toxic impacts of targeted synthesized NPs against CT-26 cancer cell line when being compared with HT-29 cells, which may be caused by the different cytotoxicity mechanisms of NPs. In addition, the targeted carriers and SPIONs were present inside and around the cells with HDM-2 expression along with only a few non-targeted vectors, while displaying no appearance throughout the normal cell.ConclusionThe results indicated the efficiency of targeted PEI-coated SPIONs for cancer diagnostic applications.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study7

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone7

In this study, PNC-27 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking PNC-27.

  1. 01LabNot a test of PNC-27 aloneIn vitro (cells)Activity measurement

    In cell tests, PNC‑27-targeted PEI-SPION carriers showed significantly higher binding to cancer cells than to NIH-3t3 cells.

    In plain wordsIn cells, PNC‑27-targeted carriers bound more to cancer cells than to NIH-3t3 cells.

    Peptide
    PNC-27
    Outcome
    IncreaseCancer cell binding
    Study

    n: not reported in source

    HT-29, CT-26 and NIH-3t3 cells
    Comparator
    NIH-3t3 cells
    Outcome group
    Receptor activity and mechanism · Cancer

    Source sentences

    Summary
    According to the cytotoxicity results, the binding and uptake abilities of the PNC-27 peptide by cancer cells were significantly higher than that of the NIH-3t3 cells.
    2 context sentences
    Summary · context
    In this work, we evaluated the in vitro potential of polyethyleneimine (PEI)-SPIONs targeted by PNC-27 peptide as a double targeting agent throughout early cancer diagnosis.
    Summary · context
    The cytotoxicity and targeting efficiency were assayed against HT-29 and CT-26 cell lines along with NIH-3t3 as normal cells by MTT method and Prussian blue staining test, respectively.

    In this study, PNC-27 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking PNC-27.

  2. 02LabNot a test of PNC-27 aloneIn vitro (cells)Activity measurement

    In cell tests, PNC‑27-targeted PEI-SPION carriers showed significantly higher uptake by cancer cells than by NIH-3t3 cells.

    In plain wordsIn cells, cancer cells took in more PNC‑27-targeted carriers than NIH-3t3 cells did.

    Peptide
    PNC-27
    Outcome
    IncreaseCancer cell uptake
    Study

    n: not reported in source

    HT-29, CT-26 and NIH-3t3 cells
    Comparator
    NIH-3t3 cells
    Outcome group
    Receptor activity and mechanism · Cancer

    Source sentences

    Summary
    According to the cytotoxicity results, the binding and uptake abilities of the PNC-27 peptide by cancer cells were significantly higher than that of the NIH-3t3 cells.
    2 context sentences
    Summary · context
    In this work, we evaluated the in vitro potential of polyethyleneimine (PEI)-SPIONs targeted by PNC-27 peptide as a double targeting agent throughout early cancer diagnosis.
    Summary · context
    The cytotoxicity and targeting efficiency were assayed against HT-29 and CT-26 cell lines along with NIH-3t3 as normal cells by MTT method and Prussian blue staining test, respectively.

    In this study, PNC-27 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking PNC-27.

  3. 03LabNot a test of PNC-27 aloneIn vitro (cells)Activity measurement

    In cell tests, PNC‑27-targeted nanoparticles caused greater toxicity in CT-26 cells than in HT-29 cells.

    In plain wordsIn cells, PNC‑27-targeted nanoparticles were more toxic to CT-26 cells than to HT-29 cells.

    Peptide
    PNC-27
    Outcome
    IncreaseNanoparticle cytotoxicity
    Study

    n: not reported in source

    HT-29, CT-26 and NIH-3t3 cells
    Comparator
    HT-29 cells
    Outcome group
    Tumor growth · Cancer

    Source sentences

    Summary
    However, the results were indicative of the more toxic impacts of targeted synthesized NPs against CT-26 cancer cell line when being compared with HT-29 cells, which may be caused by the different cytotoxicity mechanisms of NPs.
    2 context sentences
    Summary · context
    In this work, we evaluated the in vitro potential of polyethyleneimine (PEI)-SPIONs targeted by PNC-27 peptide as a double targeting agent throughout early cancer diagnosis.
    Summary · context
    The cytotoxicity and targeting efficiency were assayed against HT-29 and CT-26 cell lines along with NIH-3t3 as normal cells by MTT method and Prussian blue staining test, respectively.

    In this study, PNC-27 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking PNC-27.

  4. 04LabNot a test of PNC-27 aloneIn vitro (cells)Activity measurement

    In HDM-2-expressing cells, targeted PNC‑27 carriers and SPIONs occurred inside and around cells, whereas few non-targeted vectors occurred.

    In plain wordsIn cells expressing HDM-2, targeted carriers occurred inside and around cells, while few non-targeted vectors occurred.

    Peptide
    PNC-27
    Outcome
    IncreaseTargeted carrier localization
    Study

    n: not reported in source

    HT-29, CT-26 and NIH-3t3 cells
    Comparator
    non-targeted vectors
    Outcome group
    Receptor activity and mechanism · Cancer

    Source sentences

    Summary
    In addition, the targeted carriers and SPIONs were present inside and around the cells with HDM-2 expression along with only a few non-targeted vectors, while displaying no appearance throughout the normal cell.
    2 context sentences
    Summary · context
    In this work, we evaluated the in vitro potential of polyethyleneimine (PEI)-SPIONs targeted by PNC-27 peptide as a double targeting agent throughout early cancer diagnosis.
    Summary · context
    The cytotoxicity and targeting efficiency were assayed against HT-29 and CT-26 cell lines along with NIH-3t3 as normal cells by MTT method and Prussian blue staining test, respectively.

    In this study, PNC-27 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking PNC-27.

  5. 05LabNot a test of PNC-27 aloneIn vitro (cells)Activity measurement

    In normal cells, targeted PNC‑27 carriers and SPIONs showed no detectable appearance.

    In plain wordsIn cells, targeted PNC‑27 carriers and SPIONs did not appear in normal cells.

    Peptide
    PNC-27
    Outcome
    Not applicableCarrier localization in normal cells
    Study

    n: not reported in source

    HT-29, CT-26 and NIH-3t3 cells
    Outcome group
    Receptor activity and mechanism · Cancer

    Source sentences

    Summary
    In addition, the targeted carriers and SPIONs were present inside and around the cells with HDM-2 expression along with only a few non-targeted vectors, while displaying no appearance throughout the normal cell.
    2 context sentences
    Summary · context
    In this work, we evaluated the in vitro potential of polyethyleneimine (PEI)-SPIONs targeted by PNC-27 peptide as a double targeting agent throughout early cancer diagnosis.
    Summary · context
    The cytotoxicity and targeting efficiency were assayed against HT-29 and CT-26 cell lines along with NIH-3t3 as normal cells by MTT method and Prussian blue staining test, respectively.

    In this study, PNC-27 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking PNC-27.

  6. 06LabNot a test of PNC-27 aloneIn vitro (cell-free)Other

    In laboratory tests, PNC‑27-targeted PEI-SPION carriers had mean diameters ranging from 86.6 to 116.1 nm.

    In plain wordsIn lab tests, PNC‑27-targeted carriers had mean diameters ranging from 86.6 to 116.1 nm.

    Peptide
    PNC-27
    Outcome
    Not applicableCarrier diameter
    Study

    n: not reported in source

    synthesized PNC-27-targeted PEI-SPION carriers
    Outcome group
    Formulation, delivery and stability · Cancer

    Measurements

    Mean diameter
    86.6 to 116.1 nm
    synthesized carriers

    Source sentences

    Summary
    The mean diameter of synthesized carriers was obtained in the range of 86.6 - 116.1 nm with a positive charge.
    2 context sentences
    Summary · context
    In this work, we evaluated the in vitro potential of polyethyleneimine (PEI)-SPIONs targeted by PNC-27 peptide as a double targeting agent throughout early cancer diagnosis.
    Summary · context
    The cytotoxicity and targeting efficiency were assayed against HT-29 and CT-26 cell lines along with NIH-3t3 as normal cells by MTT method and Prussian blue staining test, respectively.

    In this study, PNC-27 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking PNC-27.

  7. 07LabNot a test of PNC-27 aloneIn vitro (cell-free)Other

    In laboratory tests, synthesized PNC‑27-targeted carriers had a positive charge.

    In plain wordsIn lab tests, PNC‑27-targeted carriers had a positive charge.

    Peptide
    PNC-27
    Outcome
    Not applicableCarrier surface charge
    Study

    n: not reported in source

    synthesized PNC-27-targeted PEI-SPION carriers
    Outcome group
    Formulation, delivery and stability · Cancer

    Source sentences

    Summary
    The mean diameter of synthesized carriers was obtained in the range of 86.6 - 116.1 nm with a positive charge.
    2 context sentences
    Summary · context
    In this work, we evaluated the in vitro potential of polyethyleneimine (PEI)-SPIONs targeted by PNC-27 peptide as a double targeting agent throughout early cancer diagnosis.
    Summary · context
    The cytotoxicity and targeting efficiency were assayed against HT-29 and CT-26 cell lines along with NIH-3t3 as normal cells by MTT method and Prussian blue staining test, respectively.

    In this study, PNC-27 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking PNC-27.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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