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Study · Animal study (in vivo) · mouse · 2022

5 results

Nicotinamide Mononucleotide Prevents Retinal Dysfunction in a Mouse Model of Retinal Ischemia/Reperfusion Injury.

Lee D, Tomita Y, Miwa Y, Shinojima A, Ban N, Yamaguchi S, Nishioka K, Negishi K, Yoshino J, Kurihara T

International journal of molecular sciences23 Sept 2022Cited by 18 (Europe PMC)

PMID36232528 (opens in a new tab)PMCIDPMC9570481 (opens in a new tab)DOI10.3390/ijms231911228 (opens in a new tab)PepBankPUB-36232528
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Summary

Retinal ischemia/reperfusion (I/R) injury can cause severe vision impairment. Retinal I/R injury is associated with pathological increases in reactive oxygen species and inflammation, resulting in retinal neuronal cell death. To date, effective therapies have not been developed. Nicotinamide mononucleotide (NMN), a key nicotinamide adenine dinucleotide (NAD+) intermediate, has been shown to exert neuroprotection for retinal diseases. However, it remains unclear whether NMN can prevent retinal I/R injury. Thus, we aimed to determine whether NMN therapy is useful for retinal I/R injury-induced retinal degeneration. One day after NMN intraperitoneal (IP) injection, adult mice were subjected to retinal I/R injury. Then, the mice were injected with NMN once every day for three days. Electroretinography and immunohistochemistry were used to measure retinal functional alterations and retinal inflammation, respectively. The protective effect of NMN administration was further examined using a retinal cell line, 661W, under CoCl2-induced oxidative stress conditions. NMN IP injection significantly suppressed retinal functional damage, as well as inflammation. NMN treatment showed protective effects against oxidative stress-induced cell death. The antioxidant pathway (Nrf2 and Hmox-1) was activated by NMN treatment. In conclusion, NMN could be a promising preventive neuroprotective drug for ischemic retinopathy.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In mice with retinal ischemia/reperfusion injury, NMN significantly suppressed retinal functional damage.

    In plain wordsIn mice with retinal injury, NMN reduced retinal functional damage.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    DecreaseRetinal functional damage
    Study

    mousen: not reported in source

    Retinal ischemia/reperfusion injury
    Outcome group
    Vision and retina · Eye
    Studied dose
    Dose not stated · intraperitoneal · One day before injury, then once daily for three daysAs given in this study (mouse).

    Source sentences

    Summary
    NMN IP injection significantly suppressed retinal functional damage, as well as inflammation.
    2 context sentences
    Summary · context
    One day after NMN intraperitoneal (IP) injection, adult mice were subjected to retinal I/R injury.
    Summary · context
    Then, the mice were injected with NMN once every day for three days.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In mice with retinal ischemia/reperfusion injury, NMN significantly suppressed inflammation.

    In plain wordsIn mice with retinal injury, NMN reduced inflammation.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    DecreaseRetinal inflammation
    Study

    mousen: not reported in source

    Retinal ischemia/reperfusion injury
    Outcome group
    Inflammation markers · Eye
    Studied dose
    Dose not stated · intraperitoneal · One day before injury, then once daily for three daysAs given in this study (mouse).

    Source sentences

    Summary
    NMN IP injection significantly suppressed retinal functional damage, as well as inflammation.
    2 context sentences
    Summary · context
    One day after NMN intraperitoneal (IP) injection, adult mice were subjected to retinal I/R injury.
    Summary · context
    Then, the mice were injected with NMN once every day for three days.
  3. 03LabIn vitro (cells)Efficacy outcome

    In retinal cells under oxidative stress, NMN protected against cell death.

    In plain wordsIn cells from a retinal cell line, NMN protected against oxidative stress-induced death.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    DecreaseCell death
    Study

    n: not reported in source

    661W retinal cells under CoCl2-induced oxidative stress
    Outcome group
    Neuroprotection · Eye
    Studied dose
    Dose not stated

    Source sentences

    Summary
    NMN treatment showed protective effects against oxidative stress-induced cell death.
    1 context sentence
    Summary · context
    The protective effect of NMN administration was further examined using a retinal cell line, 661W, under CoCl2-induced oxidative stress conditions.
  4. 04LabIn vitro (cells)Mechanism

    In retinal cell experiments, NMN activated the Nrf2 antioxidant pathway.

    In plain wordsIn lab tests, NMN activated the Nrf2 antioxidant pathway.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseNrf2 pathway activation
    Study

    n: not reported in source

    661W retinal cells under CoCl2-induced oxidative stress
    Outcome group
    Receptor activity and mechanism · Eye
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The antioxidant pathway (Nrf2 and Hmox-1) was activated by NMN treatment.
    1 context sentence
    Summary · context
    The protective effect of NMN administration was further examined using a retinal cell line, 661W, under CoCl2-induced oxidative stress conditions.
  5. 05LabIn vitro (cells)Mechanism

    In retinal cell experiments, NMN activated the Hmox-1 antioxidant pathway.

    In plain wordsIn lab tests, NMN activated the Hmox-1 antioxidant pathway.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseHmox-1 pathway activation
    Study

    n: not reported in source

    661W retinal cells under CoCl2-induced oxidative stress
    Outcome group
    Receptor activity and mechanism · Eye
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The antioxidant pathway (Nrf2 and Hmox-1) was activated by NMN treatment.
    1 context sentence
    Summary · context
    The protective effect of NMN administration was further examined using a retinal cell line, 661W, under CoCl2-induced oxidative stress conditions.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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