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Study · In vitro (cells) · 2022

5 results

Crosstalk between kisspeptin and gonadotropin-inhibitory hormone in the silence of puberty: preclinical evidence from a calcium signaling study.

Bulut F, Kacar E, Bilgin B, Hekim MG, Keleştemur MM, Sahin Z, Ayar A, Ozcan M

Journal of receptor and signal transduction research22 Sept 2022Cited by 2 (Europe PMC)

PMID36137227 (opens in a new tab)DOI10.1080/10799893.2022.2125014 (opens in a new tab)PepBankPUB-36137227
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

3 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In rHypoE-8 hypothalamic cells, kisspeptin‑10 at 300 nM significantly increased intracellular free calcium.

    In plain wordsIn cells, kisspeptin‑10 raised calcium levels inside hypothalamic cells.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseIntracellular calcium response to kisspeptin-10 at 300 nM
    Study

    n = 64

    rHypoE-8 immortalized hypothalamic cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    300 nMAs given in this study.

    Source sentences

    Summary
    Kisspeptin-10 (100, 300, and 1000 nM) caused a significant increase in [Ca2+]i in rHypoE-8 cells (n = 58, n = 64, and n = 49, respectively, p < 0.001).
    1 context sentence
    Summary · context
    Hence, this study investigated the effects of kisspeptin and GnIH on calcium signaling using immortalized hypothalamic cells (rHypoE-8) as a model.
  2. 02LabIn vitro (cells)Mechanism

    In rHypoE-8 hypothalamic cells, kisspeptin‑10 at 100 nM significantly increased intracellular free calcium.

    In plain wordsIn cells, kisspeptin‑10 raised calcium levels inside hypothalamic cells.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseIntracellular calcium response to kisspeptin-10 at 100 nM
    Study

    n = 58

    rHypoE-8 immortalized hypothalamic cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    100 nMAs given in this study.

    Source sentences

    Summary
    Kisspeptin-10 (100, 300, and 1000 nM) caused a significant increase in [Ca2+]i in rHypoE-8 cells (n = 58, n = 64, and n = 49, respectively, p < 0.001).
    1 context sentence
    Summary · context
    Hence, this study investigated the effects of kisspeptin and GnIH on calcium signaling using immortalized hypothalamic cells (rHypoE-8) as a model.
  3. 03LabIn vitro (cells)Mechanism

    In rHypoE-8 hypothalamic cells, kisspeptin‑10 at 1000 nM significantly increased intracellular free calcium.

    In plain wordsIn cells, kisspeptin‑10 raised calcium levels inside hypothalamic cells.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseIntracellular calcium response to kisspeptin-10 at 1000 nM
    Study

    n = 49

    rHypoE-8 immortalized hypothalamic cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    1000 nMAs given in this study.

    Source sentences

    Summary
    Kisspeptin-10 (100, 300, and 1000 nM) caused a significant increase in [Ca2+]i in rHypoE-8 cells (n = 58, n = 64, and n = 49, respectively, p < 0.001).
    1 context sentence
    Summary · context
    Hence, this study investigated the effects of kisspeptin and GnIH on calcium signaling using immortalized hypothalamic cells (rHypoE-8) as a model.

Studies that did not test the peptide alone2

In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Kisspeptin-10.

  1. 04LabNot a test of Kisspeptin-10 aloneIn vitro (cells)Mechanism

    In rHypoE-8 cells, GnIH attenuated the kisspeptin-evoked calcium transients. Kisspeptin served as the challenge.

    In plain wordsIn cells, GnIH reduced the calcium response to kisspeptin.

    Peptide
    Kisspeptin-10
    Outcome
    DecreaseKisspeptin-evoked calcium transients with GnIH
    Study

    n = 47

    rHypoE-8 immortalized hypothalamic cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Source sentences

    Summary
    but GnIH attenuated the kisspeptin-evoked [Ca2+]i transients (n = 47, p < 0.001).
    1 context sentence
    Summary · context
    Hence, this study investigated the effects of kisspeptin and GnIH on calcium signaling using immortalized hypothalamic cells (rHypoE-8) as a model.

    In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-10.

  2. 05LabNot a test of Kisspeptin-10 aloneIn vitro (cells)Mechanism

    In rHypoE-8 cells, the kisspeptin receptor antagonist P234 inhibited the calcium response to kisspeptin. Kisspeptin served as the challenge.

    In plain wordsIn cells, a kisspeptin receptor blocker reduced the calcium response to kisspeptin.

    Peptide
    Kisspeptin-10
    Outcome
    DecreaseKisspeptin-evoked calcium response with antagonist P234
    Study

    n = 32

    rHypoE-8 immortalized hypothalamic cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Source sentences

    Summary
    The kisspeptin receptor antagonist, P234, inhibited the calcium responses to kisspeptin (p n = 32).
    1 context sentence
    Summary · context
    Hence, this study investigated the effects of kisspeptin and GnIH on calcium signaling using immortalized hypothalamic cells (rHypoE-8) as a model.

    In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-10.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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