Kisspeptin-10 in cryodiluent improves the post-thaw quality of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa.

Fayyaz MH, Andrabi SMH, Haider MS, Khalique MA, Shah SAH

Andrologia19 Aug 2022Cited by 2 (Europe PMC)

PMID36054451DOI10.1111/and.14564PepBankPUB-36054451

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

10 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueEfficacy outcome
In frozen-thawed buffalo bull sperm, 20 μmol/L kisspeptin‑10 in the cryodiluent gave the highest catalase level versus the negative control.
In plain wordsIn lab tests on buffalo sperm, kisspeptin‑10 in the freezing medium gave higher catalase than medium without it.
Peptide
Kisspeptin-10
Outcome
IncreasePost-thaw catalase level
Study
buffalo (Bubalus bubalis)n: not reported in source
cryopreserved buffalo bull semen
Comparator
Negative control (cryodiluent without kisspeptin-10)
Outcome group
Oxidative stress · Reproductive and sexual health
Studied dose
20 μmol/L · added to cryodiluentAs given in this study (buffalo (Bubalus bubalis)).
Source sentences
Summary
At post-thawing, catalase (unit mg^-1 ), peroxidase (unit mg^-1 ) and reduced glutathione (μmol L^-1 ) levels were highest (p -1 of kisspeptin-10 as compared to negative control.
1 context sentence(design, population, supporting numbers)
Summary · context
Qualified semen samples from five bulls were pooled, divided into five aliquots and extended in Tris-citric acid cryodiluent containing differential doses of kisspeptin-10 (5, 10, 15, and 20 μmol L^-1 and negative control.
Report a problemFND-3605445101
02LabEx vivo tissueEfficacy outcome
In frozen-thawed buffalo bull sperm, 20 μmol/L kisspeptin‑10 gave the highest peroxidase level versus the negative control.
In plain wordsIn lab tests on buffalo sperm, kisspeptin‑10 in the freezing medium gave higher peroxidase than medium without it.
Peptide
Kisspeptin-10
Outcome
IncreasePost-thaw peroxidase level
Study
buffalo (Bubalus bubalis)n: not reported in source
cryopreserved buffalo bull semen
Comparator
Negative control (cryodiluent without kisspeptin-10)
Outcome group
Oxidative stress · Reproductive and sexual health
Studied dose
20 μmol/L · added to cryodiluentAs given in this study (buffalo (Bubalus bubalis)).
Source sentences
Summary
At post-thawing, catalase (unit mg^-1 ), peroxidase (unit mg^-1 ) and reduced glutathione (μmol L^-1 ) levels were highest (p -1 of kisspeptin-10 as compared to negative control.
1 context sentence(design, population, supporting numbers)
Summary · context
Qualified semen samples from five bulls were pooled, divided into five aliquots and extended in Tris-citric acid cryodiluent containing differential doses of kisspeptin-10 (5, 10, 15, and 20 μmol L^-1 and negative control.
Report a problemFND-3605445102
03LabEx vivo tissueEfficacy outcome
In frozen-thawed buffalo bull sperm, 20 μmol/L kisspeptin‑10 gave the highest reduced glutathione level versus the negative control.
In plain wordsIn lab tests on buffalo sperm, kisspeptin‑10 in the freezing medium gave higher glutathione than medium without it.
Peptide
Kisspeptin-10
Outcome
IncreasePost-thaw reduced glutathione level
Study
buffalo (Bubalus bubalis)n: not reported in source
cryopreserved buffalo bull semen
Comparator
Negative control (cryodiluent without kisspeptin-10)
Outcome group
Oxidative stress · Reproductive and sexual health
Studied dose
20 μmol/L · added to cryodiluentAs given in this study (buffalo (Bubalus bubalis)).
Source sentences
Summary
At post-thawing, catalase (unit mg^-1 ), peroxidase (unit mg^-1 ) and reduced glutathione (μmol L^-1 ) levels were highest (p -1 of kisspeptin-10 as compared to negative control.
1 context sentence(design, population, supporting numbers)
Summary · context
Qualified semen samples from five bulls were pooled, divided into five aliquots and extended in Tris-citric acid cryodiluent containing differential doses of kisspeptin-10 (5, 10, 15, and 20 μmol L^-1 and negative control.
Report a problemFND-3605445103
04LabEx vivo tissueEfficacy outcome
In frozen-thawed buffalo bull sperm, 20 μmol/L kisspeptin‑10 gave the lowest lipid peroxidation versus the negative control.
In plain wordsIn lab tests on buffalo sperm, kisspeptin‑10 in the freezing medium gave less lipid damage than medium without it.
Peptide
Kisspeptin-10
Outcome
DecreasePost-thaw lipid peroxidation
Study
buffalo (Bubalus bubalis)n: not reported in source
cryopreserved buffalo bull semen
Comparator
Negative control (cryodiluent without kisspeptin-10)
Outcome group
Oxidative stress · Reproductive and sexual health
Studied dose
20 μmol/L · added to cryodiluentAs given in this study (buffalo (Bubalus bubalis)).
Source sentences
Summary
Moreover, lipid peroxidation (nmol L^-1 min^-1 mg protein^-1 ) level was lowest (p -1 of kisspeptin-10.
1 context sentence(design, population, supporting numbers)
Summary · context
Qualified semen samples from five bulls were pooled, divided into five aliquots and extended in Tris-citric acid cryodiluent containing differential doses of kisspeptin-10 (5, 10, 15, and 20 μmol L^-1 and negative control.
Report a problemFND-3605445104
05LabEx vivo tissueEfficacy outcome
In frozen-thawed buffalo bull sperm, 15 and 20 μmol/L kisspeptin‑10 raised progressive motility versus the negative control.
In plain wordsIn lab tests on buffalo sperm, kisspeptin‑10 in the freezing medium kept more sperm moving forward than medium without it.
Peptide
Kisspeptin-10
Outcome
IncreasePost-thaw progressive motility
Study
buffalo (Bubalus bubalis)n: not reported in source
cryopreserved buffalo bull semen
Comparator
Negative control (cryodiluent without kisspeptin-10)
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
15 and 20 μmol/L · added to cryodiluentAs given in this study (buffalo (Bubalus bubalis)).
Source sentences
Summary
Sperm progressive motility (%), rapid velocity (%) and kinematics were higher (p -1 of kisspeptin-10 as compared to negative control.
1 context sentence(design, population, supporting numbers)
Summary · context
Qualified semen samples from five bulls were pooled, divided into five aliquots and extended in Tris-citric acid cryodiluent containing differential doses of kisspeptin-10 (5, 10, 15, and 20 μmol L^-1 and negative control.
Report a problemFND-3605445105
06LabEx vivo tissueEfficacy outcome
In frozen-thawed buffalo bull sperm, 15 and 20 μmol/L kisspeptin‑10 raised the percentage of rapidly moving sperm versus the negative control.
In plain wordsIn lab tests on buffalo sperm, kisspeptin‑10 in the freezing medium gave more fast sperm than medium without it.
Peptide
Kisspeptin-10
Outcome
IncreasePost-thaw rapid velocity
Study
buffalo (Bubalus bubalis)n: not reported in source
cryopreserved buffalo bull semen
Comparator
Negative control (cryodiluent without kisspeptin-10)
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
15 and 20 μmol/L · added to cryodiluentAs given in this study (buffalo (Bubalus bubalis)).
Source sentences
Summary
Sperm progressive motility (%), rapid velocity (%) and kinematics were higher (p -1 of kisspeptin-10 as compared to negative control.
1 context sentence(design, population, supporting numbers)
Summary · context
Qualified semen samples from five bulls were pooled, divided into five aliquots and extended in Tris-citric acid cryodiluent containing differential doses of kisspeptin-10 (5, 10, 15, and 20 μmol L^-1 and negative control.
Report a problemFND-3605445106
07LabEx vivo tissueEfficacy outcome
In frozen-thawed buffalo bull sperm, 15 and 20 μmol/L kisspeptin‑10 improved sperm kinematics versus the negative control.
In plain wordsIn lab tests on buffalo sperm, kisspeptin‑10 in the freezing medium improved sperm movement measures compared with medium without it.
Peptide
Kisspeptin-10
Outcome
IncreasePost-thaw sperm kinematics
Study
buffalo (Bubalus bubalis)n: not reported in source
cryopreserved buffalo bull semen
Comparator
Negative control (cryodiluent without kisspeptin-10)
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
15 and 20 μmol/L · added to cryodiluentAs given in this study (buffalo (Bubalus bubalis)).
Source sentences
Summary
Sperm progressive motility (%), rapid velocity (%) and kinematics were higher (p -1 of kisspeptin-10 as compared to negative control.
1 context sentence(design, population, supporting numbers)
Summary · context
Qualified semen samples from five bulls were pooled, divided into five aliquots and extended in Tris-citric acid cryodiluent containing differential doses of kisspeptin-10 (5, 10, 15, and 20 μmol L^-1 and negative control.
Report a problemFND-3605445107
08LabEx vivo tissueEfficacy outcome
In frozen-thawed buffalo bull sperm, all kisspeptin‑10 doses improved supra-vital plasma membrane integrity versus the negative control.
In plain wordsIn lab tests on buffalo sperm, every kisspeptin‑10 dose kept more sperm membranes intact than medium without it.
Peptide
Kisspeptin-10
Outcome
IncreasePost-thaw plasma membrane integrity
Study
buffalo (Bubalus bubalis)n: not reported in source
cryopreserved buffalo bull semen
Comparator
Negative control (cryodiluent without kisspeptin-10)
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
5, 10, 15 and 20 μmol/L · added to cryodiluentAs given in this study (buffalo (Bubalus bubalis)).
Source sentences
Summary
Supra-vital plasma membrane integrity (%), viable sperm with intact acrosome (%) and DNA integrity (%) were improved (p < 0.05) with all doses of kisspeptin-10 as compared to negative control.
1 context sentence(design, population, supporting numbers)
Summary · context
Qualified semen samples from five bulls were pooled, divided into five aliquots and extended in Tris-citric acid cryodiluent containing differential doses of kisspeptin-10 (5, 10, 15, and 20 μmol L^-1 and negative control.
Report a problemFND-3605445108
09LabEx vivo tissueEfficacy outcome
In frozen-thawed buffalo bull sperm, all kisspeptin‑10 doses increased viable sperm with intact acrosome versus the negative control.
In plain wordsIn lab tests on buffalo sperm, every kisspeptin‑10 dose kept more sperm with intact acrosome than medium without it.
Peptide
Kisspeptin-10
Outcome
IncreasePost-thaw viable sperm with intact acrosome
Study
buffalo (Bubalus bubalis)n: not reported in source
cryopreserved buffalo bull semen
Comparator
Negative control (cryodiluent without kisspeptin-10)
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
5, 10, 15 and 20 μmol/L · added to cryodiluentAs given in this study (buffalo (Bubalus bubalis)).
Source sentences
Summary
Supra-vital plasma membrane integrity (%), viable sperm with intact acrosome (%) and DNA integrity (%) were improved (p < 0.05) with all doses of kisspeptin-10 as compared to negative control.
1 context sentence(design, population, supporting numbers)
Summary · context
Qualified semen samples from five bulls were pooled, divided into five aliquots and extended in Tris-citric acid cryodiluent containing differential doses of kisspeptin-10 (5, 10, 15, and 20 μmol L^-1 and negative control.
Report a problemFND-3605445109
10LabEx vivo tissueEfficacy outcome
In frozen-thawed buffalo bull sperm, all kisspeptin‑10 doses improved DNA integrity versus the negative control.
In plain wordsIn lab tests on buffalo sperm, every kisspeptin‑10 dose kept sperm DNA more intact than medium without it.
Peptide
Kisspeptin-10
Outcome
IncreasePost-thaw DNA integrity
Study
buffalo (Bubalus bubalis)n: not reported in source
cryopreserved buffalo bull semen
Comparator
Negative control (cryodiluent without kisspeptin-10)
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
5, 10, 15 and 20 μmol/L · added to cryodiluentAs given in this study (buffalo (Bubalus bubalis)).
Source sentences
Summary
Supra-vital plasma membrane integrity (%), viable sperm with intact acrosome (%) and DNA integrity (%) were improved (p < 0.05) with all doses of kisspeptin-10 as compared to negative control.
1 context sentence(design, population, supporting numbers)
Summary · context
Qualified semen samples from five bulls were pooled, divided into five aliquots and extended in Tris-citric acid cryodiluent containing differential doses of kisspeptin-10 (5, 10, 15, and 20 μmol L^-1 and negative control.
Report a problemFND-3605445110

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

Subjects

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Kisspeptin-10 in cryodiluent improves the post-thaw quality of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa.

Summary

Results from this study10

Version history

Subjects

Publication types

MeSH terms

Keywords