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Study · Animal study (in vivo) · mouse · 2022

2 results

Systems spatiotemporal dynamics of traumatic brain injury at single-cell resolution reveals humanin as a therapeutic target.

Arneson D, Zhang G, Ahn IS, Ying Z, Diamante G, Cely I, Palafox-Sanchez V, Gomez-Pinilla F, Yang X

Cellular and molecular life sciences : CMLS11 Aug 2022Cited by 36 (Europe PMC)

PMID35951114 (opens in a new tab)PMCIDPMC9372016 (opens in a new tab)DOI10.1007/s00018-022-04495-9 (opens in a new tab)PepBankPUB-35951114
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Summary

BackgroundThe etiology of mild traumatic brain injury (mTBI) remains elusive due to the tissue and cellular heterogeneity of the affected brain regions that underlie cognitive impairments and subsequent neurological disorders. This complexity is further exacerbated by disrupted circuits within and between cell populations across brain regions and the periphery, which occur at different timescales and in spatial domains.MethodsWe profiled three tissues (hippocampus, frontal cortex, and blood leukocytes) at the acute (24-h) and subacute (7-day) phases of mTBI at single-cell resolution.ResultsWe demonstrated that the coordinated gene expression patterns across cell types were disrupted and re-organized by TBI at different timescales with distinct regional and cellular patterns. Gene expression-based network modeling implied astrocytes as a key regulator of the cell-cell coordination following mTBI in both hippocampus and frontal cortex across timepoints, and mt-Rnr2, which encodes the mitochondrial peptide humanin, as a potential target for intervention based on its broad regional and dynamic dysregulation following mTBI. Treatment of a murine mTBI model with humanin reversed cognitive impairment caused by mTBI through the restoration of metabolic pathways within astrocytes.ConclusionsOur results offer a systems-level understanding of the dynamic and spatial regulation of gene programs by mTBI and pinpoint key target genes, pathways, and cell circuits that are amenable to therapeutics.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study2

1 result from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In a murine model of mild traumatic brain injury, humanin treatment reversed cognitive impairment through restoration of metabolic pathways within astrocytes.

    In plain wordsIn mice with mild brain injury, humanin treatment reversed memory and thinking problems.

    Peptide
    Humanin
    Outcome
    DecreaseCognitive impairment after mTBI
    Study

    mousen: not reported in source

    murine mild traumatic brain injury model
    Outcome group
    Cognition and memory · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Treatment of a murine mTBI model with humanin reversed cognitive impairment caused by mTBI through the restoration of metabolic pathways within astrocytes.

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 02AnimalEndogenous levelAnimal study (in vivo)Mechanism

    After mild traumatic brain injury, expression of mt-Rnr2, the gene that encodes humanin, was dysregulated across brain regions and timepoints.

    In plain wordsIn mice with mild brain injury, the gene that makes the body's own humanin was dysregulated in many brain areas.

    Peptide
    Humanin
    Outcome
    Mixedmt-Rnr2 (humanin gene) expression after mTBI
    Study

    mousen: not reported in source

    murine mild traumatic brain injury (single-cell profiling of hippocampus and frontal cortex)
    Outcome group
    Neuroprotection · Nervous system and cognition

    Source sentences

    Summary
    and mt-Rnr2, which encodes the mitochondrial peptide humanin, as a potential target for intervention based on its broad regional and dynamic dysregulation following mTBI.
    1 context sentence
    Summary · context
    We profiled three tissues (hippocampus, frontal cortex, and blood leukocytes) at the acute (24-h) and subacute (7-day) phases of mTBI at single-cell resolution.

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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