Quantitative Acetylomics Reveals Dynamics of Protein Lysine Acetylation in Mouse Livers During Aging and Upon the Treatment of Nicotinamide Mononucleotide.

Li J, Cao Y, Niu K, Qiu J, Wang H, You Y, Li D, Luo Y, Zhu Z, Zhang Y, Liu N

Molecular & cellular proteomics : MCP3 Aug 2022Cited by 11 (Europe PMC)

PMID35931320PMCIDPMC9436820DOI10.1016/j.mcpro.2022.100276PepBankPUB-35931320

Summary

Lysine acetylation is a reversible and dynamic post-translational modification that plays vital roles in regulating multiple cellular processes including aging. However, acetylome-wide analysis in the aging process remains poorly studied in mammalian tissues. Nicotinamide adenine dinucleotide (NAD⁺), a hub metabolite, benefits health span at least in part due to the activation of Sirtuins, a family of NAD⁺-consuming deacetylases, indicating changes in acetylome. Here, we combine two antibodies for the enrichment of acetylated peptides and perform label-free quantitative acetylomic analysis of mouse livers during natural aging and upon the treatment of beta-nicotinamide mononucleotide (NMN), a NAD⁺ booster. Our study describes previously unknown acetylation sites and reveals the acetylome-wide dynamics with age as well as upon the treatment of NMN. We discover protein acetylation events as potential aging biomarkers. We demonstrate that the life-beneficial effect of NMN could be partially reflected by the changes in age-related protein acetylation. Our quantitative assessment indicates that NMN has mild effects on acetylation sites previously reported as substrates of Sirtuins. Collectively, our data analyze protein acetylation with age, laying critical foundation for the functional study of protein post-translational modification essential for healthy aging and perhaps disease conditions.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Mechanism
In mouse livers, NMN treatment changed age-related protein acetylation.
In plain wordsIn mice, NMN changed age-related protein acetylation in the liver.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Direction not reportedAge-related protein acetylation
Study
mousen: not reported in source
mouse liver during aging
Outcome group
Aging markers · Liver
Studied dose
Dose not stated
Source sentences
Summary
Our study describes previously unknown acetylation sites and reveals the acetylome-wide dynamics with age as well as upon the treatment of NMN.
2 context sentences(design, population, supporting numbers)
Summary · context
We demonstrate that the life-beneficial effect of NMN could be partially reflected by the changes in age-related protein acetylation.
Summary · context
Here, we combine two antibodies for the enrichment of acetylated peptides and perform label-free quantitative acetylomic analysis of mouse livers during natural aging and upon the treatment of beta-nicotinamide mononucleotide (NMN), a NAD⁺ booster.
Show in the study text Report a problemFND-3593132001
02AnimalAnimal study (in vivo)Mechanism
In mouse livers, NMN had mild effects on acetylation sites previously reported as Sirtuin substrates.
In plain wordsIn mice, NMN had mild effects on liver acetylation sites reported as Sirtuin targets.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Direction not reportedAcetylation of reported Sirtuin substrates
Study
mousen: not reported in source
mouse liver during aging
Outcome group
Aging markers · Liver
Studied dose
Dose not stated
Source sentences
Summary
Our quantitative assessment indicates that NMN has mild effects on acetylation sites previously reported as substrates of Sirtuins.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we combine two antibodies for the enrichment of acetylated peptides and perform label-free quantitative acetylomic analysis of mouse livers during natural aging and upon the treatment of beta-nicotinamide mononucleotide (NMN), a NAD⁺ booster.
Show in the study text Report a problemFND-3593132002

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

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