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Study · In vitro (cells) · 2022

6 results

Nicotinamide Mononucleotide and Coenzyme Q10 Protects Fibroblast Senescence Induced by Particulate Matter Preconditioned Mast Cells.

Chang TM, Yang TY, Huang HC

International journal of molecular sciences7 Jul 2022Cited by 15 (Europe PMC)

PMID35886889 (opens in a new tab)PMCIDPMC9319393 (opens in a new tab)DOI10.3390/ijms23147539 (opens in a new tab)PepBankPUB-35886889
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Summary

Particulate matter (PM) pollutants impose a certain degree of destruction and toxicity to the skin. Mast cells in the skin dermis could be activated by PMs that diffuse across the blood vessel after being inhaled. Mast cell degranulation in the dermis provides a kind of inflammatory insult to local fibroblasts. In this study, we evaluated human dermal fibroblast responses to conditioned medium from KU812 cells primed with PM. We found that PM promoted the production of proinflammatory cytokines in mast cells and that the cell secretome induced reactive oxygen species and mitochondrial reactive oxygen species production in dermal fibroblasts. Nicotinamide mononucleotide or coenzyme Q10 alleviated the generation of excessive ROS and mitochondrial ROS induced by the conditioned medium from PM-activated KU812 cells. PM-conditioned medium treatment increased the NF-κB expression in dermal fibroblasts, whereas NMN or Q10 inhibited p65 upregulation by PM. The reduced sirtuin 1 (SIRT 1) and nuclear factor erythroid 2-related Factor 2 (Nrf2) expression induced by PM-conditioned medium was reversed by NMN or Q10 in HDFs. Moreover, NMN or Q10 attenuated the expression of senescent β-galactosidase induced by PM-conditioned KU812 cell medium. These findings suggest that NMN or Q10 ameliorates PM-induced inflammation by improving the cellular oxidative status, suppressing proinflammatory NF-κB, and promoting the levels of the antioxidant and anti-inflammatory regulators Nrf2 and SIRT1 in HDFs. The present observations help to understand the factors that affect HDFs in the dermal microenvironment and the therapeutic role of NMN and Q10 as suppressors of skin aging.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In human dermal fibroblasts, NMN reduced excessive ROS generation induced by medium from PM-activated KU812 cells.

    In plain wordsIn cells, NMN reduced reactive oxygen species induced by medium from particle-exposed mast cells.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    DecreaseReactive oxygen species generation
    Study

    humann: not reported in source

    human dermal fibroblasts exposed to conditioned medium from PM-primed KU812 cells
    Outcome group
    Aging markers · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Nicotinamide mononucleotide or coenzyme Q10 alleviated the generation of excessive ROS and mitochondrial ROS induced by the conditioned medium from PM-activated KU812 cells.
    1 context sentence
    Summary · context
    In this study, we evaluated human dermal fibroblast responses to conditioned medium from KU812 cells primed with PM.
  2. 02LabIn vitro (cells)Mechanism

    In human dermal fibroblasts, NMN reduced mitochondrial ROS induced by medium from PM-activated KU812 cells.

    In plain wordsIn cells, NMN reduced mitochondrial reactive oxygen species induced by medium from particle-exposed mast cells.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    DecreaseMitochondrial ROS generation
    Study

    humann: not reported in source

    human dermal fibroblasts exposed to conditioned medium from PM-primed KU812 cells
    Outcome group
    Mitochondrial function · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Nicotinamide mononucleotide or coenzyme Q10 alleviated the generation of excessive ROS and mitochondrial ROS induced by the conditioned medium from PM-activated KU812 cells.
    1 context sentence
    Summary · context
    In this study, we evaluated human dermal fibroblast responses to conditioned medium from KU812 cells primed with PM.
  3. 03LabIn vitro (cells)Mechanism

    In human dermal fibroblasts, NMN inhibited p65 upregulation induced by PM-conditioned medium.

    In plain wordsIn cells, NMN reduced p65 upregulation caused by particle-conditioned medium.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    Decreasep65 upregulation
    Study

    humann: not reported in source

    human dermal fibroblasts exposed to conditioned medium from PM-primed KU812 cells
    Outcome group
    Inflammation markers · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    PM-conditioned medium treatment increased the NF-κB expression in dermal fibroblasts, whereas NMN or Q10 inhibited p65 upregulation by PM.
    1 context sentence
    Summary · context
    In this study, we evaluated human dermal fibroblast responses to conditioned medium from KU812 cells primed with PM.
  4. 04LabIn vitro (cells)Mechanism

    In human dermal fibroblasts, NMN reversed the reduction in SIRT1 expression induced by PM-conditioned medium.

    In plain wordsIn cells, NMN reversed the reduction in SIRT1 expression caused by particle-conditioned medium.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseSIRT1 expression
    Study

    humann: not reported in source

    human dermal fibroblasts exposed to conditioned medium from PM-primed KU812 cells
    Outcome group
    Aging markers · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The reduced sirtuin 1 (SIRT 1) and nuclear factor erythroid 2-related Factor 2 (Nrf2) expression induced by PM-conditioned medium was reversed by NMN or Q10 in HDFs.
    1 context sentence
    Summary · context
    In this study, we evaluated human dermal fibroblast responses to conditioned medium from KU812 cells primed with PM.
  5. 05LabIn vitro (cells)Mechanism

    In human dermal fibroblasts, NMN reversed the reduction in Nrf2 expression induced by PM-conditioned medium.

    In plain wordsIn cells, NMN reversed the reduction in Nrf2 expression caused by particle-conditioned medium.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseNrf2 expression
    Study

    humann: not reported in source

    human dermal fibroblasts exposed to conditioned medium from PM-primed KU812 cells
    Outcome group
    Aging markers · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The reduced sirtuin 1 (SIRT 1) and nuclear factor erythroid 2-related Factor 2 (Nrf2) expression induced by PM-conditioned medium was reversed by NMN or Q10 in HDFs.
    1 context sentence
    Summary · context
    In this study, we evaluated human dermal fibroblast responses to conditioned medium from KU812 cells primed with PM.
  6. 06LabIn vitro (cells)Mechanism

    In human dermal fibroblasts, NMN attenuated senescent β-galactosidase expression induced by PM-conditioned KU812 medium.

    In plain wordsIn cells, NMN reduced senescent β-galactosidase expression caused by particle-conditioned mast cell medium.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    DecreaseSenescent β-galactosidase expression
    Study

    humann: not reported in source

    human dermal fibroblasts exposed to conditioned medium from PM-primed KU812 cells
    Outcome group
    Aging markers · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Moreover, NMN or Q10 attenuated the expression of senescent β-galactosidase induced by PM-conditioned KU812 cell medium.
    1 context sentence
    Summary · context
    In this study, we evaluated human dermal fibroblast responses to conditioned medium from KU812 cells primed with PM.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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