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Study · In vitro (cells) · 2022

5 results

KISS1R and ANKRD31 Cooperate to Enhance Leydig Cell Gene Expression via the Cytoskeletal-Nucleoskeletal Pathway.

Ricci G, Guillou F, Catizone A, Mele VG, Moggio M, Chioccarelli T, Diano N, Meccariello R, Pierantoni R, Fasano S, Cobellis G, Chianese R, Manfrevola F

Frontiers in cell and developmental biology23 Jun 2022Cited by 1 (Europe PMC)

PMID35813201 (opens in a new tab)PMCIDPMC9260857 (opens in a new tab)DOI10.3389/fcell.2022.877270 (opens in a new tab)PepBankPUB-35813201
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Summary

Kisspeptins are involved in the regulation of hypothalamic-pituitary-gonadal axis, Leydig cell functions, and testosterone secretion, acting as endogenous ligands of the KISS1 receptor. ANKRD31 protein participates in male fertility, regulating meiotic progression, and epididymal sperm maturation. Here, we show that in Leydig cells, KISS1 receptor and ANKRD31 proteins physically interact; the formation of this protein complex is enhanced by Kisspeptin-10 that also modulates F-actin synthesis, favoring histone acetylation in chromatin and gene expression via the cytoskeletal-nucleoskeletal pathway. Kp/KISS1R system deregulation, expression impairment of cytoskeletal-nucleoskeletal mediators, Leydig gene targets, and the decreased testosterone secretion in Ankrd31 -/- testis strongly supported our hypothesis. Furthermore, cytochalasin D treatment subverted the gene expression induction dependent on Kisspeptin-10 action. In conclusion, the current work highlights a novel role for the Kisspeptin-10 in the induction of the cytoskeletal-nucleoskeletal route, downstream a physical interaction between KISS1 receptor and ANKRD31, with gene expression activation as final effect, in Leydig cells.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study5

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In Leydig cells, kisspeptin‑10 enhanced the formation of the KISS1 receptor and ANKRD31 protein complex.

    In plain wordsIn cells, kisspeptin‑10 strengthened the link between the kisspeptin receptor and the ANKRD31 protein in Leydig cells.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseKISS1R-ANKRD31 complex formation
    Study

    n: not reported in source

    Leydig cells
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    in Leydig cells, KISS1 receptor and ANKRD31 proteins physically interact; the formation of this protein complex is enhanced by Kisspeptin-10
  2. 02LabIn vitro (cells)Mechanism

    In Leydig cells, kisspeptin‑10 modulated F-actin synthesis. The direction is not stated.

    In plain wordsIn cells, kisspeptin‑10 changed F-actin production in Leydig cells.

    Peptide
    Kisspeptin-10
    Outcome
    Direction not reportedF-actin synthesis
    Study

    n: not reported in source

    Leydig cells
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Kisspeptin-10 that also modulates F-actin synthesis
  3. 03LabIn vitro (cells)Mechanism

    In Leydig cells, kisspeptin‑10 favored histone acetylation in chromatin.

    In plain wordsIn cells, kisspeptin‑10 promoted histone acetylation in Leydig cells.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseHistone acetylation in chromatin
    Study

    n: not reported in source

    Leydig cells
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    favoring histone acetylation in chromatin and gene expression via the cytoskeletal-nucleoskeletal pathway.
  4. 04LabIn vitro (cells)Mechanism

    In Leydig cells, kisspeptin‑10 activated gene expression via the cytoskeletal-nucleoskeletal pathway.

    In plain wordsIn cells, kisspeptin‑10 switched on gene activity in Leydig cells through the cell's actin skeleton pathway.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseLeydig cell gene expression via the cytoskeletal-nucleoskeletal pathway
    Study

    n: not reported in source

    Leydig cells
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    highlights a novel role for the Kisspeptin-10 in the induction of the cytoskeletal-nucleoskeletal route, downstream a physical interaction between KISS1 receptor and ANKRD31, with gene expression activation as final effect, in Leydig cells.

Studies that did not test the peptide alone1

In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Kisspeptin-10.

  1. 05LabNot a test of Kisspeptin-10 aloneIn vitro (cells)Mechanism

    In Leydig cells, cytochalasin D treatment subverted the induction of gene expression that depended on kisspeptin‑10. The regimen has two active compounds.

    In plain wordsIn cells, the actin blocker cytochalasin D stopped kisspeptin‑10 from inducing gene activity in Leydig cells.

    Peptide
    Kisspeptin-10
    Outcome
    DecreaseKisspeptin-10-dependent gene expression induction with cytochalasin D
    Study

    n: not reported in source

    Leydig cells
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health

    Source sentences

    Summary
    Furthermore, cytochalasin D treatment subverted the gene expression induction dependent on Kisspeptin-10 action.

    In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-10.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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