Administration of nicotinamide mononucleotide improves oocyte quality of obese mice.

Wang L, Chen Y, Wei J, Guo F, Li L, Han Z, Wang Z, Zhu H, Zhang X, Li Z, Dai X

Cell proliferation10 Jul 2022Cited by 50 (Europe PMC)

PMID35811338PMCIDPMC9628229DOI10.1111/cpr.13303PepBankPUB-35811338

Summary

ObjectivesObesity has become a common health concern around the world. Maternal obesity could cause poor reproductive outcomes due to chronic ovarian inflammation and decreased oocyte quality. However, the strategies to improve the poor reproductive outcomes of obese females have not been fully studied. In this study, we aimed to explore the effects and underlying mechanisms of nicotinamide mononucleotide (NMN) on oocyte quality and reproductive performance of obese mice.Materials and methodsThe obese mouse model was established by feeding high-fat diet which was confirmed by body weight record, fasting blood glucose test and oral glucose tolerance test. The expression of ovary development related genes and inflammation related genes, including Lhx8, Bmp4, Adgre1, Ccl2, TNF-α, Gal-3, Clec10a and IL-10 in ovaries and the expression of Bax and Sod1 in oocytes were detected using quantitative reverse transcription PCR (RT-qPCR). The adipose size of abdominal fat tissue was determined with haematoxylin and eosin (H&E) staining. Immunofluorescence staining was performed to measure the ROS level, spindle/chromosome structure, mitochondrial function, actin dynamics and DNA damage of oocytes.ResultsThe administration of NMN restored ovarian weight and reduced the adipose size of abdominal fat tissue and ovarian inflammation in high fat diet (HFD) mice. Furthermore, NMN treatment improved the oocytes quality partially by restoring the mitochondrial function and actin dynamics, reducing meiotic defects, DNA damage and ROS level and lipid droplet distribution of oocytes in HFD mice. On the long-term effect, NMN restored offspring body weight of HFD mice.ConclusionNMN could improve the oocyte quality of HFD-induced obese mice.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study11

11 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In obese mice, NMN restored ovarian weight.
In plain wordsIn mice with obesity, NMN restored ovarian weight.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Direction not reportedOvarian weight
Study
mousen: not reported in source
high-fat-diet obese mice
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
The administration of NMN restored ovarian weight and reduced the adipose size of abdominal fat tissue and ovarian inflammation in high fat diet (HFD) mice.
1 context sentence(design, population, supporting numbers)
Summary · context
The obese mouse model was established by feeding high-fat diet which was confirmed by body weight record, fasting blood glucose test and oral glucose tolerance test.
Show in the study text Report a problemFND-3581133801
02AnimalAnimal study (in vivo)Efficacy outcome
In obese mice, NMN reduced abdominal adipose size.
In plain wordsIn mice with obesity, NMN reduced abdominal fat tissue size.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseAbdominal adipose size
Study
mousen: not reported in source
high-fat-diet obese mice
Outcome group
Body weight · Metabolic and weight
Studied dose
Dose not stated
Source sentences
Summary
The administration of NMN restored ovarian weight and reduced the adipose size of abdominal fat tissue and ovarian inflammation in high fat diet (HFD) mice.
1 context sentence(design, population, supporting numbers)
Summary · context
The obese mouse model was established by feeding high-fat diet which was confirmed by body weight record, fasting blood glucose test and oral glucose tolerance test.
Show in the study text Report a problemFND-3581133802
03AnimalAnimal study (in vivo)Efficacy outcome
In obese mice, NMN reduced ovarian inflammation.
In plain wordsIn mice with obesity, NMN reduced ovarian inflammation.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseOvarian inflammation
Study
mousen: not reported in source
high-fat-diet obese mice
Outcome group
Inflammation markers · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
The administration of NMN restored ovarian weight and reduced the adipose size of abdominal fat tissue and ovarian inflammation in high fat diet (HFD) mice.
1 context sentence(design, population, supporting numbers)
Summary · context
The obese mouse model was established by feeding high-fat diet which was confirmed by body weight record, fasting blood glucose test and oral glucose tolerance test.
Show in the study text Report a problemFND-3581133803
04AnimalAnimal study (in vivo)Efficacy outcome
In obese mice, NMN improved oocyte quality.
In plain wordsIn mice with obesity, NMN improved egg quality.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseOocyte quality
Study
mousen: not reported in source
high-fat-diet obese mice
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, NMN treatment improved the oocytes quality partially by restoring the mitochondrial function and actin dynamics, reducing meiotic defects, DNA damage and ROS level and lipid droplet distribution of oocytes in HFD mice.
1 context sentence(design, population, supporting numbers)
Summary · context
The obese mouse model was established by feeding high-fat diet which was confirmed by body weight record, fasting blood glucose test and oral glucose tolerance test.
Show in the study text Report a problemFND-3581133804
05AnimalAnimal study (in vivo)Efficacy outcome
In obese mice, NMN restored oocyte mitochondrial function.
In plain wordsIn mice with obesity, NMN restored mitochondrial function in eggs.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Direction not reportedOocyte mitochondrial function
Study
mousen: not reported in source
high-fat-diet obese mice
Outcome group
Mitochondrial function · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, NMN treatment improved the oocytes quality partially by restoring the mitochondrial function and actin dynamics, reducing meiotic defects, DNA damage and ROS level and lipid droplet distribution of oocytes in HFD mice.
1 context sentence(design, population, supporting numbers)
Summary · context
The obese mouse model was established by feeding high-fat diet which was confirmed by body weight record, fasting blood glucose test and oral glucose tolerance test.
Show in the study text Report a problemFND-3581133805
06AnimalAnimal study (in vivo)Efficacy outcome
In obese mice, NMN restored oocyte actin dynamics.
In plain wordsIn mice with obesity, NMN restored actin dynamics in eggs.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Direction not reportedOocyte actin dynamics
Study
mousen: not reported in source
high-fat-diet obese mice
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, NMN treatment improved the oocytes quality partially by restoring the mitochondrial function and actin dynamics, reducing meiotic defects, DNA damage and ROS level and lipid droplet distribution of oocytes in HFD mice.
1 context sentence(design, population, supporting numbers)
Summary · context
The obese mouse model was established by feeding high-fat diet which was confirmed by body weight record, fasting blood glucose test and oral glucose tolerance test.
Show in the study text Report a problemFND-3581133806
07AnimalAnimal study (in vivo)Efficacy outcome
In obese mice, NMN reduced oocyte meiotic defects.
In plain wordsIn mice with obesity, NMN reduced meiotic defects in eggs.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseOocyte meiotic defects
Study
mousen: not reported in source
high-fat-diet obese mice
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, NMN treatment improved the oocytes quality partially by restoring the mitochondrial function and actin dynamics, reducing meiotic defects, DNA damage and ROS level and lipid droplet distribution of oocytes in HFD mice.
1 context sentence(design, population, supporting numbers)
Summary · context
The obese mouse model was established by feeding high-fat diet which was confirmed by body weight record, fasting blood glucose test and oral glucose tolerance test.
Show in the study text Report a problemFND-3581133807
08AnimalAnimal study (in vivo)Efficacy outcome
In obese mice, NMN reduced oocyte DNA damage.
In plain wordsIn mice with obesity, NMN reduced DNA damage in eggs.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseOocyte DNA damage
Study
mousen: not reported in source
high-fat-diet obese mice
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, NMN treatment improved the oocytes quality partially by restoring the mitochondrial function and actin dynamics, reducing meiotic defects, DNA damage and ROS level and lipid droplet distribution of oocytes in HFD mice.
1 context sentence(design, population, supporting numbers)
Summary · context
The obese mouse model was established by feeding high-fat diet which was confirmed by body weight record, fasting blood glucose test and oral glucose tolerance test.
Show in the study text Report a problemFND-3581133808
09AnimalAnimal study (in vivo)Efficacy outcome
In obese mice, NMN reduced oocyte ROS levels.
In plain wordsIn mice with obesity, NMN reduced reactive oxygen species in eggs.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseOocyte ROS levels
Study
mousen: not reported in source
high-fat-diet obese mice
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, NMN treatment improved the oocytes quality partially by restoring the mitochondrial function and actin dynamics, reducing meiotic defects, DNA damage and ROS level and lipid droplet distribution of oocytes in HFD mice.
1 context sentence(design, population, supporting numbers)
Summary · context
The obese mouse model was established by feeding high-fat diet which was confirmed by body weight record, fasting blood glucose test and oral glucose tolerance test.
Show in the study text Report a problemFND-3581133809
10AnimalAnimal study (in vivo)Efficacy outcome
In obese mice, NMN reduced oocyte lipid droplet distribution.
In plain wordsIn mice with obesity, NMN reduced lipid droplet distribution in eggs.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseOocyte lipid droplet distribution
Study
mousen: not reported in source
high-fat-diet obese mice
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, NMN treatment improved the oocytes quality partially by restoring the mitochondrial function and actin dynamics, reducing meiotic defects, DNA damage and ROS level and lipid droplet distribution of oocytes in HFD mice.
1 context sentence(design, population, supporting numbers)
Summary · context
The obese mouse model was established by feeding high-fat diet which was confirmed by body weight record, fasting blood glucose test and oral glucose tolerance test.
Show in the study text Report a problemFND-3581133810
11AnimalAnimal study (in vivo)Efficacy outcome
In offspring of obese mice, maternal NMN treatment restored body weight over the long term.
In plain wordsIn mice, maternal NMN treatment restored offspring body weight over the long term.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Direction not reportedOffspring body weight
Study
mousen: not reported in source
high-fat-diet obese mice
Outcome group
Body weight · Metabolic and weight
Studied dose
Dose not stated
Source sentences
Summary
On the long-term effect, NMN restored offspring body weight of HFD mice.
1 context sentence(design, population, supporting numbers)
Summary · context
The obese mouse model was established by feeding high-fat diet which was confirmed by body weight record, fasting blood glucose test and oral glucose tolerance test.
Show in the study text Report a problemFND-3581133811

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

MeSH terms

PepBank is a research reference. Talk to a doctor before you use any peptide.