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Study · In vitro (cells) · pig · 2022

2 results

Nicotinamide mononucleotide supplementation improves the quality of porcine oocytes under heat stress.

Song M, Li Y, Zhou Y, Yan J, Zhou X, Gao Q, Miao Y, Xiong B

Journal of animal science and biotechnology15 Jun 2022Cited by 25 (Europe PMC)

PMID35706001 (opens in a new tab)PMCIDPMC9202089 (opens in a new tab)DOI10.1186/s40104-022-00716-0 (opens in a new tab)PepBankPUB-35706001
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Summary

BackgroundElevated ambient temperature-caused heat stress is a major concern for livestock production due to its negative impact on animal feed intake, growth, reproduction, and health. Particularly, the germ cells are extremely sensitive to the heat stress. However, the effective approach and strategy regarding how to protect mammalian oocytes from heat stress-induced defects have not been determined.MethodsGerminal vesicle (GV) porcine oocytes were cultured at 41.5 °C for 24 h to induce heat stress, and then cultured at 38.5 °C to the specific developmental stage for subsequent analysis. Nicotinamide mononucleotide (NMN) was dissolved in water to 1 mol/L for a stock solution and further diluted with the maturation medium to the final concentrations of 10 μmol/L, 20 μmol/L, 50 μmol/L or 100 μmol/L, respectively, during heat stress. Immunostaining and fluorescence intensity quantification were applied to assess the effects of heat stress and NMN supplementation on the key processes during the oocyte meiotic maturation.ResultsHere, we report that NMN supplementation improves the quality of porcine oocytes under heat stress. Specifically, we found that heat stress resulted in oocyte maturation failure by disturbing the dynamics of meiotic organelles, including the cytoskeleton assembly, cortical granule distribution and mitochondrial function. In addition, heat stress induced the production of excessive reactive oxygen species (ROS) and DNA damage, leading to the occurrence of apoptosis in oocytes and subsequent embryonic development arrest. More importantly, we validated that supplementation of NMN during heat stress restored the meiotic defects during porcine oocyte maturation.ConclusionsTaken together, our study documents that NMN supplementation is an effective approach to improve the quality of oocytes under heat stress by promoting both nuclear and cytoplasmic maturation.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In cultured porcine oocytes under heat stress, NMN improved oocyte quality.

    In plain wordsIn lab tests, NMN improved the quality of pig oocytes under heat stress.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseOocyte quality
    Study

    pign: not reported in source

    porcine oocytes under heat stress
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    10, 20, 50 or 100 μmol/L · during heat stressAs given in this study (pig).

    Source sentences

    Summary
    Here, we report that NMN supplementation improves the quality of porcine oocytes under heat stress.
    1 context sentence
    Summary · context
    Germinal vesicle (GV) porcine oocytes were cultured at 41.5 °C for 24 h to induce heat stress, and then cultured at 38.5 °C to the specific developmental stage for subsequent analysis.
  2. 02LabIn vitro (cells)Efficacy outcome

    In cultured porcine oocytes, NMN during heat stress restored meiotic defects.

    In plain wordsIn lab tests, NMN reduced meiotic defects in pig oocytes under heat stress.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    DecreaseMeiotic defects
    Study

    pign: not reported in source

    porcine oocytes under heat stress
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    10, 20, 50 or 100 μmol/L · during heat stressAs given in this study (pig).

    Source sentences

    Summary
    More importantly, we validated that supplementation of NMN during heat stress restored the meiotic defects during porcine oocyte maturation.
    1 context sentence
    Summary · context
    Germinal vesicle (GV) porcine oocytes were cultured at 41.5 °C for 24 h to induce heat stress, and then cultured at 38.5 °C to the specific developmental stage for subsequent analysis.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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