Effects of Thymosin β4 on Myocardial Apoptosis in Burned Rats.

Wu X, Li S, Feng X, Wen H, Meng X, Sun K

Journal of healthcare engineering3 Mar 2022Cited by 1 (Europe PMC)

PMID35281544PMCIDPMC8913053DOI10.1155/2022/2129629PepBankPUB-35281544

Summary

The aim of this study was to investigate the effects of thymosin β4 on myocardial apoptosis following burns. Fifty healthy Sprague Dawley (SD) rats were randomly divided into the normal control group, resuscitation group the low-dose Tβ4 (thymosin β4) group (2g), the medium-dose Tβ4 group (6g), and the high-dose Tβ4 group (18g). The rats were immersed in 95°C hot water for 18 seconds, and then the model of 30% body surface area (TBSA) III° scald was established. The resuscated rats were injected with lactate Ringer's solution for antishock rehydration, while the Tβ4 treatment group was injected with lactate Ringer's solution for antishock rehydration, and the animals were sacrificed 6 h after scald. The degree of histopathological damage was observed by HE (hematoxylin and eosin) staining. Western blot was used to detect STAT1 and STAT3 protein expression levels. Real-time PCR was used to detect mRNA expressions of STAT1 and STAT3. The results showed that the apoptosis rate of the resuscitation group was significantly higher than that of the control group (P < 0.01). Compared with the resuscitation group, the apoptosis rate of thymosin β4 in the treatment group was significantly reduced (P < 0.01). Compared with the normal control group, the expression of STAT1 protein was increased and the expression of STAT3 protein was decreased in model group rats after ischemia and reperfusion. Compared with the model group, the expression of STAT1 protein decreased and the expression of STAT3 protein increased after ischemia-reperfusion in the thymosin β4 treatment group. Thymosin β4 may protect the myocardium by downregulating STAT1 and upregulating STAT3 expression and inhibiting myocardial apoptosis induced by ischemia and reperfusion after severe scald injury.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study3

3 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In burned rats, Tβ4 treatment significantly reduced the myocardial apoptosis rate compared with the resuscitation group.
In plain wordsIn burned rats, Tβ4 lowered heart cell death compared with rats that only received fluids.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseMyocardial apoptosis rate
Study
ratn: not reported in source
30% TBSA III degree scald with ischemia and reperfusion
Comparator
Resuscitation group
Outcome group
Myocardial injury · Cardiovascular
Studied dose
low 2g, medium 6g, high 18gAs given in this study (rat).
Source sentences
Summary
Compared with the resuscitation group, the apoptosis rate of thymosin β4 in the treatment group was significantly reduced (P < 0.01).
2 context sentences(design, population, supporting numbers)
Summary · context
Fifty healthy Sprague Dawley (SD) rats were randomly divided into the normal control group, resuscitation group the low-dose Tβ4 (thymosin β4) group (2g), the medium-dose Tβ4 group (6g), and the high-dose Tβ4 group (18g).
Summary · context
The rats were immersed in 95°C hot water for 18 seconds, and then the model of 30% body surface area (TBSA) III° scald was established.
Show in the study text Report a problemFND-3528154401
02AnimalAnimal study (in vivo)Mechanism
In burned rats, Tβ4 treatment decreased STAT1 protein expression compared with the model group.
In plain wordsIn burned rats, Tβ4 lowered STAT1 protein compared with model rats.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseSTAT1 protein expression
Study
ratn: not reported in source
30% TBSA III degree scald with ischemia and reperfusion
Comparator
Resuscitation group
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
low 2g, medium 6g, high 18gAs given in this study (rat).
Source sentences
Summary
Compared with the model group, the expression of STAT1 protein decreased and the expression of STAT3 protein increased after ischemia-reperfusion in the thymosin β4 treatment group.
2 context sentences(design, population, supporting numbers)
Summary · context
Fifty healthy Sprague Dawley (SD) rats were randomly divided into the normal control group, resuscitation group the low-dose Tβ4 (thymosin β4) group (2g), the medium-dose Tβ4 group (6g), and the high-dose Tβ4 group (18g).
Summary · context
The rats were immersed in 95°C hot water for 18 seconds, and then the model of 30% body surface area (TBSA) III° scald was established.
Show in the study text Report a problemFND-3528154402
03AnimalAnimal study (in vivo)Mechanism
In burned rats, Tβ4 treatment increased STAT3 protein expression compared with the model group.
In plain wordsIn burned rats, Tβ4 raised STAT3 protein compared with model rats.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseSTAT3 protein expression
Study
ratn: not reported in source
30% TBSA III degree scald with ischemia and reperfusion
Comparator
Resuscitation group
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
low 2g, medium 6g, high 18gAs given in this study (rat).
Source sentences
Summary
Compared with the model group, the expression of STAT1 protein decreased and the expression of STAT3 protein increased after ischemia-reperfusion in the thymosin β4 treatment group.
2 context sentences(design, population, supporting numbers)
Summary · context
Fifty healthy Sprague Dawley (SD) rats were randomly divided into the normal control group, resuscitation group the low-dose Tβ4 (thymosin β4) group (2g), the medium-dose Tβ4 group (6g), and the high-dose Tβ4 group (18g).
Summary · context
The rats were immersed in 95°C hot water for 18 seconds, and then the model of 30% body surface area (TBSA) III° scald was established.
Show in the study text Report a problemFND-3528154403

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 01:52 UTC
Study results updated
Version 1
12 Oct 2026, 18:17 UTC
First added · source update from Europe PMC

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