Immunocastration with gene vaccine (KISS1) induces a cell-mediated immune response in ram testis: A transcriptome evaluation.

Ahmed S, Bo D, Zhao J, Liu G, Ding Y, Jiang X, Wassie T, Jing H

Reproduction in domestic animals = Zuchthygiene20 Mar 2022Cited by 9 (Europe PMC)

PMID35247007DOI10.1111/rda.14106PepBankPUB-35247007

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

No result from this study comes from a test of the peptide.

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01AnimalEndogenous levelAnimal study (in vivo)Other
In rams, KISS1 gene vaccine immunization lowered serum levels of the body's own kisspeptin‑54.
In plain wordsIn rams, a KISS1 gene vaccine lowered the body's own kisspeptin‑54 levels.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreaseKisspeptin-54 level
Study
sheep (ram)n: not reported in source
rams immunized with intranasal KISS1 gene vaccine
Comparator
Non-immunized control rams
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
The results showed that we successfully constructed the KISS1 immune castration ram model, in which testicular growth and development, testosterone and kisspeptin-54 levels, and sexual function were suppressed in immunized rams (p < .05).
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, we used ram as a model animal to identify the cellular immune response in testicular tissues of rams immunized with intranasal KISS1 gene vaccine.
Summary · context
The immune castration model was evaluated by sexual behaviours, spermatogenesis and serum hormone profiles after the KISS1 gene immunization.
This result measures the body's own Kisspeptin-54 (metastin). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-3524700703

Studies that did not test the peptide alone5

In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Kisspeptin-54 (metastin).

02AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Efficacy outcome
In rams, KISS1 gene vaccine immunization suppressed testicular growth and development.
In plain wordsIn rams, a KISS1 gene vaccine slowed testicle growth and development.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreaseTesticular growth and development
Study
sheep (ram)n: not reported in source
rams immunized with intranasal KISS1 gene vaccine
Comparator
Non-immunized control rams
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
The results showed that we successfully constructed the KISS1 immune castration ram model, in which testicular growth and development, testosterone and kisspeptin-54 levels, and sexual function were suppressed in immunized rams (p < .05).
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, we used ram as a model animal to identify the cellular immune response in testicular tissues of rams immunized with intranasal KISS1 gene vaccine.
Summary · context
The immune castration model was evaluated by sexual behaviours, spermatogenesis and serum hormone profiles after the KISS1 gene immunization.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3524700701
03AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Efficacy outcome
In rams, KISS1 gene vaccine immunization lowered serum testosterone.
In plain wordsIn rams, a KISS1 gene vaccine lowered testosterone.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreaseTestosterone level
Study
sheep (ram)n: not reported in source
rams immunized with intranasal KISS1 gene vaccine
Comparator
Non-immunized control rams
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
The results showed that we successfully constructed the KISS1 immune castration ram model, in which testicular growth and development, testosterone and kisspeptin-54 levels, and sexual function were suppressed in immunized rams (p < .05).
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, we used ram as a model animal to identify the cellular immune response in testicular tissues of rams immunized with intranasal KISS1 gene vaccine.
Summary · context
The immune castration model was evaluated by sexual behaviours, spermatogenesis and serum hormone profiles after the KISS1 gene immunization.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3524700702
04AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Efficacy outcome
In rams, KISS1 gene vaccine immunization suppressed sexual function.
In plain wordsIn rams, a KISS1 gene vaccine reduced sexual function.
Peptide
Kisspeptin-54 (metastin)
Outcome
DecreaseSexual function
Study
sheep (ram)n: not reported in source
rams immunized with intranasal KISS1 gene vaccine
Comparator
Non-immunized control rams
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
The results showed that we successfully constructed the KISS1 immune castration ram model, in which testicular growth and development, testosterone and kisspeptin-54 levels, and sexual function were suppressed in immunized rams (p < .05).
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, we used ram as a model animal to identify the cellular immune response in testicular tissues of rams immunized with intranasal KISS1 gene vaccine.
Summary · context
The immune castration model was evaluated by sexual behaviours, spermatogenesis and serum hormone profiles after the KISS1 gene immunization.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3524700704
05AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In rams, KISS1 gene vaccine immunization changed 21 cellular-immunity genes in testis, 14 up and seven down.
In plain wordsIn rams, a KISS1 gene vaccine changed 21 immune-related genes in the testis: 14 went up and seven went down.
Peptide
Kisspeptin-54 (metastin)
Outcome
MixedDifferentially expressed cellular-immunity genes in testis
Study
sheep (ram)n: not reported in source
rams immunized with intranasal KISS1 gene vaccine
Comparator
Non-immunized control rams
Outcome group
Immune response · Immune and inflammation
Measurements
differentially expressed genes
21 genes
testis, immunized vs control
upregulated genes
14 genes
testis
Source sentences
Summary
Using Hiseq™ 2000 high sequencing for ram testicular, we identified 21 differentially expressed genes (DEGs) related to cellular immunity, of which, 14 genes were upregulated and seven genes were downregulated in the testis of the immunized group (p < .05).
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, we used ram as a model animal to identify the cellular immune response in testicular tissues of rams immunized with intranasal KISS1 gene vaccine.
Summary · context
The immune castration model was evaluated by sexual behaviours, spermatogenesis and serum hormone profiles after the KISS1 gene immunization.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3524700705
06AnimalNot a test of Kisspeptin-54 (metastin) aloneAnimal study (in vivo)Mechanism
In ram testis after KISS1 gene vaccination, differentially expressed genes were enriched in MHC class I antigen presentation and natural killer cell cytotoxic pathways.
In plain wordsIn rams, a KISS1 gene vaccine changed testis genes tied to antigen presentation and natural killer cell killing.
Peptide
Kisspeptin-54 (metastin)
Outcome
Not applicablePathway enrichment of differentially expressed genes
Study
sheep (ram)n: not reported in source
rams immunized with intranasal KISS1 gene vaccine
Comparator
Non-immunized control rams
Outcome group
Immune response · Immune and inflammation
Source sentences
Summary
The Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment showed that these differentially expressed genes were enriched in the antigen presentation process mediated by MHC class I and the cytotoxic pathway mediated by natural killer cells.
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, we used ram as a model animal to identify the cellular immune response in testicular tissues of rams immunized with intranasal KISS1 gene vaccine.
Summary · context
The immune castration model was evaluated by sexual behaviours, spermatogenesis and serum hormone profiles after the KISS1 gene immunization.
In this study, Kisspeptin-54 (metastin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-54 (metastin).
Report a problemFND-3524700706

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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