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Study · In vitro (cells) · cattle · 2022

6 results

Kisspeptin-10 Promotes Progesterone Synthesis in Bovine Ovarian Granulosa Cells via Downregulation of microRNA-1246.

Guo L, Xu H, Li Y, Liu H, Zhao J, Lu W, Wang J

Genes3 Feb 2022Cited by 10 (Europe PMC)

PMID35205342 (opens in a new tab)PMCIDPMC8871966 (opens in a new tab)DOI10.3390/genes13020298 (opens in a new tab)PepBankPUB-35205342
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Summary

The objective of this study was to clarify the effect of kisspeptin-10 (kp-10) on the synthesis of progesterone (P4) in bovine granulosa cells (BGCs) and its mechanisms via microRNA 1246 (miR-1246). According to the results, we found that treating with kp-10 for 24 h could increase P4 level, the mRNA expression of the steroidogenesis-related gene steroidogenic acute regulatory protein (StAR), free cholesterol content, and decrease miR-1246 expression in BGCs. Overexpression of miR-1246 significantly inhibited P4 synthesis, StAR mRNA expression, and free cholesterol content in BGCs, whereas underexpression of miR-1246 significantly reversed this effect in BGCs. Additionally, overexpression of miR-1246 counteracted the accelerative effect of kp-10 on P4 synthesis, StAR mRNA expression, and free cholesterol content in BGCs. Conversely, underexpression of miR-1246 enhanced the accelerative effect of kp-10 on P4 synthesis, StAR mRNA expression, and free cholesterol content in BGCs. Meanwhile, results of dual-luciferase reporter assays indicated that miR-1246 targeted the 3'UTR of StAR in BGCs. These results demonstrated that kp-10 induced P4 synthesis in BGCs by promoting free cholesterol transport via regulating expression of miR-1246/StAR.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study6

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In bovine granulosa cells, treatment with kp-10 for 24 h increased progesterone level.

    In plain wordsIn cow ovarian cells in the lab, kisspeptin‑10 increased progesterone levels.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseProgesterone (P4) level
    Study

    cattlen: not reported in source

    bovine ovarian granulosa cells
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated · 24 hAs given in this study (cattle).

    Source sentences

    Summary
    treating with kp-10 for 24 h could increase P4 level, the mRNA expression of the steroidogenesis-related gene steroidogenic acute regulatory protein (StAR), free cholesterol content, and decrease miR-1246 expression in BGCs.
    1 context sentence
    Summary · context
    The objective of this study was to clarify the effect of kisspeptin-10 (kp-10) on the synthesis of progesterone (P4) in bovine granulosa cells (BGCs) and its mechanisms via microRNA 1246 (miR-1246).
  2. 02LabIn vitro (cells)Mechanism

    In bovine granulosa cells, kp-10 for 24 h increased mRNA expression of StAR.

    In plain wordsIn cow ovarian cells in the lab, kisspeptin‑10 increased activity of a gene needed for progesterone production.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseStAR mRNA expression
    Study

    cattlen: not reported in source

    bovine ovarian granulosa cells
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated · 24 hAs given in this study (cattle).

    Source sentences

    Summary
    treating with kp-10 for 24 h could increase P4 level, the mRNA expression of the steroidogenesis-related gene steroidogenic acute regulatory protein (StAR)
    1 context sentence
    Summary · context
    The objective of this study was to clarify the effect of kisspeptin-10 (kp-10) on the synthesis of progesterone (P4) in bovine granulosa cells (BGCs) and its mechanisms via microRNA 1246 (miR-1246).
  3. 03LabIn vitro (cells)Mechanism

    In bovine granulosa cells, kp-10 for 24 h increased free cholesterol content.

    In plain wordsIn cow ovarian cells in the lab, kisspeptin‑10 increased free cholesterol, a building block for progesterone.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseFree cholesterol content
    Study

    cattlen: not reported in source

    bovine ovarian granulosa cells
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated · 24 hAs given in this study (cattle).

    Source sentences

    Summary
    treating with kp-10 for 24 h could increase P4 level, the mRNA expression of the steroidogenesis-related gene steroidogenic acute regulatory protein (StAR), free cholesterol content
    1 context sentence
    Summary · context
    The objective of this study was to clarify the effect of kisspeptin-10 (kp-10) on the synthesis of progesterone (P4) in bovine granulosa cells (BGCs) and its mechanisms via microRNA 1246 (miR-1246).
  4. 04LabIn vitro (cells)Mechanism

    In bovine granulosa cells, kp-10 for 24 h decreased miR-1246 expression.

    In plain wordsIn cow ovarian cells in the lab, kisspeptin‑10 lowered the level of a small regulatory RNA, miR-1246.

    Peptide
    Kisspeptin-10
    Outcome
    DecreasemiR-1246 expression
    Study

    cattlen: not reported in source

    bovine ovarian granulosa cells
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated · 24 hAs given in this study (cattle).

    Source sentences

    Summary
    treating with kp-10 for 24 h could increase P4 level, the mRNA expression of the steroidogenesis-related gene steroidogenic acute regulatory protein (StAR), free cholesterol content, and decrease miR-1246 expression in BGCs
    1 context sentence
    Summary · context
    The objective of this study was to clarify the effect of kisspeptin-10 (kp-10) on the synthesis of progesterone (P4) in bovine granulosa cells (BGCs) and its mechanisms via microRNA 1246 (miR-1246).

Studies that did not test the peptide alone2

In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Kisspeptin-10.

  1. 05LabNot a test of Kisspeptin-10 aloneIn vitro (cells)Mechanism

    In bovine granulosa cells, overexpression of miR-1246 counteracted the kp-10 increase in P4 synthesis, StAR mRNA expression and free cholesterol content.

    In plain wordsIn cow ovarian cells in the lab, raising miR-1246 blocked the progesterone-boosting effects of kisspeptin‑10.

    Peptide
    Kisspeptin-10
    Outcome
    DecreasemiR-1246 overexpression effect on kp-10 action
    Study

    cattlen: not reported in source

    bovine ovarian granulosa cells
    Comparator
    kp-10 alone
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health

    Source sentences

    Summary
    Additionally, overexpression of miR-1246 counteracted the accelerative effect of kp-10 on P4 synthesis, StAR mRNA expression, and free cholesterol content in BGCs.
    1 context sentence
    Summary · context
    The objective of this study was to clarify the effect of kisspeptin-10 (kp-10) on the synthesis of progesterone (P4) in bovine granulosa cells (BGCs) and its mechanisms via microRNA 1246 (miR-1246).

    In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-10.

  2. 06LabNot a test of Kisspeptin-10 aloneIn vitro (cells)Mechanism

    In bovine granulosa cells, underexpression of miR-1246 enhanced the kp-10 increase in P4 synthesis, StAR mRNA expression and free cholesterol content.

    In plain wordsIn cow ovarian cells in the lab, lowering miR-1246 made the progesterone-boosting effects of kisspeptin‑10 stronger.

    Peptide
    Kisspeptin-10
    Outcome
    IncreasemiR-1246 underexpression effect on kp-10 action
    Study

    cattlen: not reported in source

    bovine ovarian granulosa cells
    Comparator
    kp-10 alone
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health

    Source sentences

    Summary
    Conversely, underexpression of miR-1246 enhanced the accelerative effect of kp-10 on P4 synthesis, StAR mRNA expression, and free cholesterol content in BGCs.
    1 context sentence
    Summary · context
    The objective of this study was to clarify the effect of kisspeptin-10 (kp-10) on the synthesis of progesterone (P4) in bovine granulosa cells (BGCs) and its mechanisms via microRNA 1246 (miR-1246).

    In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-10.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    9 Oct 2026, 18:17 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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