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Study · In vitro (cells) · rainbow trout · 2022

4 results

Functional characterization of melanocortin-3 receptor in rainbow trout (Oncorhynchus mykiss).

Yu HX, Li Y, Song WJ, Wang H, Mo HL, Liu Q, Zhang XM, Jiang ZB, Wang LX

Fish physiology and biochemistry31 Jan 2022Cited by 11 (Europe PMC)

PMID35098384 (opens in a new tab)DOI10.1007/s10695-021-01033-5 (opens in a new tab)PepBankPUB-35098384
Source linkPubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

3 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In cells expressing rainbow trout MC3R, NDP-MSH activated the receptor and increased intracellular cAMP production.

    In plain wordsIn cells with the rainbow trout MC3R receptor, NDP-MSH activated the receptor and raised cAMP.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseIntracellular cAMP production via rtMC3R
    Study

    rainbow troutn: not reported in source

    rtMC3R luciferase reporter assays
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Four agonists such as α-MSH, β-MSH, ACTH (1-24), and NDP-MSH can active rtMC3R, increasing the production of intracellular cAMP and upregulating MAPK/ERK signals.
    1 context sentence
    Summary · context
    The cellular function of rtMC3R was further verified by the signal-pathway-specific luciferase reporter assays.
  2. 02LabIn vitro (cells)Activity measurement

    In cells expressing rainbow trout MC3R, NDP-MSH activated the receptor and upregulated MAPK/ERK signals.

    In plain wordsIn cells with the rainbow trout MC3R receptor, NDP-MSH activated the receptor and raised MAPK/ERK signals.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseMAPK/ERK signaling via rtMC3R
    Study

    rainbow troutn: not reported in source

    rtMC3R luciferase reporter assays
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Four agonists such as α-MSH, β-MSH, ACTH (1-24), and NDP-MSH can active rtMC3R, increasing the production of intracellular cAMP and upregulating MAPK/ERK signals.
    1 context sentence
    Summary · context
    The cellular function of rtMC3R was further verified by the signal-pathway-specific luciferase reporter assays.
  3. 03LabIn vitro (cells)Mechanism

    In cells expressing rainbow trout MC3R, stimulation with α-MSH and NDP-MSH significantly inhibited the NF-κB signaling pathway.

    In plain wordsIn cells with the rainbow trout MC3R receptor, NDP-MSH and α-MSH reduced NF-κB signaling.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    DecreaseNF-κB signaling pathway
    Study

    rainbow troutn: not reported in source

    rtMC3R luciferase reporter assays
    Outcome group
    Receptor activity and mechanism · Immune and inflammation
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Moreover, we found that rtMC3R stimulated with α-MSH and NDP-MSH can significantly inhibit the NF-κB signaling pathway.
    1 context sentence
    Summary · context
    The cellular function of rtMC3R was further verified by the signal-pathway-specific luciferase reporter assays.

Studies that did not test the peptide alone1

In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Melanotan I (afamelanotide).

  1. 04AnimalNot a test of Melanotan I (afamelanotide) aloneAnimal study (in vivo)Mechanism

    In rainbow trout, qPCR showed that rtMC3R transcripts were highly expressed in the brain and muscle.

    In plain wordsIn rainbow trout, the MC3R receptor gene was highly expressed in the brain and muscle.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicablertMC3R transcript expression by tissue
    Study

    rainbow troutn: not reported in source

    tissue qPCR
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Quantitative PCR (qPCR) revealed that the transcripts of rtMC3R were highly expressed in the brain and muscle.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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